Sporotrichosis Caused by Sporothrix mexicana, Portugal

Dias, Nicolina; Oliveira, Manoel Marques Evangelista; Portela, Maria Gabriella Sá Araújo; Santos, Cledir; Zancopé‐Oliveira, Rosely Maria; Lima, Nelson

doi:10.3201/eid1710.110737_article.htm

Cited by 13 publications

(21 citation statements)

References 6 publications

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“…On the other hand, S. brasiliensis was believed to be restricted to Brazil [6,8,9] and S. mexicana to environmental samples from Mexico [6]. Recently, however, S. mexicana was found in a human patient in Portugal [10] and in re-identification of three clinical isolates maintained in fungal collections since 1955 [9]. Taxonomic revision of S. schenckii var.…”

Section: Introductionmentioning

confidence: 97%

Development and optimization of a new MALDI-TOF protocol for identification of the Sporothrix species complex

Oliveira

Santos

Sampaio

et al. 2015

Research in Microbiology

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Accurate species identification of the Sporothrix schenckii complex is essential, since identification based only on phenotypic characteristics is often inconclusive due to phenotypic variability within the species. We used matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for species identification of 70 environmental and clinical isolates of the Sporothrix complex. A reference database was established for MALDI-TOF MS-based species identification according to minor adjustments in the manufacturer's guidelines. The MALDI-TOF MS clearly distinguished strains of Sporothrix brasiliensis, Sporothrix globosa, Sporothrix mexicana, S. schenckii, Sporothrix luriei and Sporothrix pallida, enabling identification of all isolates at the species level, as confirmed by partial calmodulin gene sequence analyses. The present methodology is simple, reliable, rapid and highly suitable for routine identification in clinical mycology laboratories and culture collections, particularly for updating and reclassifying of deposited Sporothrix isolates.

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Section: Introductionmentioning

confidence: 97%

Development and optimization of a new MALDI-TOF protocol for identification of the Sporothrix species complex

Oliveira

Santos

Sampaio

et al. 2015

Research in Microbiology

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“…Emerging and re‐emerging fungal pathogens have been continuously detected and described (Dias et al . ; Santos et al . ).…”

Section: Introductionmentioning

confidence: 99%

Synthesis, characterization and antifungal activity of chemically and fungal-produced silver nanoparticles against Trichophyton rubrum

et al. 2014

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“…Among them, S. brasiliensis type strain CBS 120339 (IPEC16490), S. globosa IPEC27135 (18), S. schenckii IPEC29334 (IOC1226) (19), and S. mexicana (MUM11.02) (3) were used as controls. All 35 strains were previously phenotypically characterized (3,19), and 15 isolates could not be identified (Table 1).Genomic DNA was extracted from the mycelial phase (25), and PCR was performed with the primer T3B (5=-AGGTCGCGGGT TCGAATCC-3=) (24). T3B PCR fingerprinting reproducibility was confirmed by repeating the assays at least 3 times under the same conditions at two different laboratories.…”

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Rapid Identification of Sporothrix Species by T3B Fingerprinting

et al. 2012

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bThis article describes PCR fingerprinting using the universal primer T3B to distinguish among species of the Sporothrix complex, S. brasiliensis, S. globosa, S. mexicana, and S. schenckii. This methodology generated distinct banding patterns, allowing the correct identification of all 35 clinical isolates at the species level, confirmed by partial calmodulin (CAL) gene sequence analyses. This methodology is simple, reliable, rapid, and cheap, making it an ideal routine identification system for clinical mycology laboratories. Sporotrichosis is a globally distributed subcutaneous mycosis with areas of high endemicity (11,21) that is caused by the dimorphic fungus Sporothrix schenckii (22). Sporotrichosis has been regarded as a job-related disease occurring as isolated cases or small outbreaks affecting people exposed to plants or soil (1, 6, 7). Rio de Janeiro State, Brazil, is a region of sporotrichosis hyperendemicity where several human and animal cases have been described since 1998 (5, 23).The diagnosis of sporotrichosis is attained by clinical, epidemiological, and laboratorial data, including culture and analysis of phenotypic characteristics. The first description of PCR for sporotrichosis' diagnosis was reported in 2001 (9). A diagnostic nested PCR assay targeting the S. schenckii 18S rRNA gene was further evaluated and showed high sensitivity and specificity, indicating that PCR may be clinically useful for diagnosis (8).S. schenckii was long considered a single taxon, although great genetic variation within this species has been described (10). By associating phenotypic and genotypic features, Marimon et al. (13) recognized three new species, Sporothrix brasiliensis, Sporothrix globosa, and Sporothrix mexicana, and proposed an identification key for these Sporothrix species. S. globosa has worldwide distribution (12, 18), whereas S. brasiliensis is apparently restricted to Brazil (13) and S. mexicana to Mexican environmental samples (13), although the latter was recently identified in Portugal (3). Additionally, these authors have proposed the promotion of S. schenckii var. luriei to the status of a new species, Sporothrix luriei (14). However, identification based only on phenotypic characteristics is often inconclusive due to phenotypic variability within these species. Therefore, new rapid and reliable identification strategies are necessary (19).Analysis of tRNA intergenic spacers was first used to distinguish Streptococcus species (15). It has also been applied successfully for Candida identification (2,16,24). Here, we evaluate T3B PCR fingerprinting to differentiate clinical Sporothrix strains at the species level in comparison to analysis of partial calmodulin (CAL) gene sequences (19).Thirty-five Sporothrix spp. isolates from the Fungal Culture Collection of IPEC/Fiocruz were included in this study approved by the Ethics Commission of the same institution. Among them, S. brasiliensis type strain CBS 120339 (IPEC16490), S. globosa IPEC27135 (18), S. schenckii IPEC29334 (IOC1226) (19), and S. m...

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Sporotrichosis Caused by Sporothrix mexicana, Portugal

Cited by 13 publications

References 6 publications

Development and optimization of a new MALDI-TOF protocol for identification of the Sporothrix species complex

Development and optimization of a new MALDI-TOF protocol for identification of the Sporothrix species complex

Synthesis, characterization and antifungal activity of chemically and fungal-produced silver nanoparticles against Trichophyton rubrum

Rapid Identification of Sporothrix Species by T3B Fingerprinting

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