Recent studies have shown that lower-limb plyometric training can effectively enhance muscle strength and explosiveness, which are particularly important for improving jumping ability. The purpose of this study was to evaluate the effects of plyometric training on vertical, lateral, and horizontal jumping abilities, and their subsequent impact on basketball shooting performance and sports injury prevention. A quasi-experimental design was used, recruiting 30 male college-level basketball players from Taiwan, who were randomly assigned to an experimental group (n = 15) and a control group (n = 15). Both groups participated in 2 h of basketball training daily, while the experimental group additionally engaged in plyometric training twice a week. The results revealed significant improvements in the experimental group in several key areas, including rate of force development (RFD), ground reaction force (GRF), jump height, jump distance, and both horizontal and vertical forces, across vertical, lateral, and horizontal jumps. Specifically, vertical jumps required the highest ground reaction force, followed by lateral jumps, with horizontal (step-back) jumps requiring the least. The optimal angles for the resultant force during take-off were found to be between 66.1° and 66.8° for lateral jumps, and between 56.2° and 57.2° for step-back jumps, while vertical jumps did not show significant variation in take-off angle. In terms of basketball performance, the experimental group demonstrated significantly better post-test results in all three types of jump shots, with the highest accuracy observed in the vertical jump shot, followed by the lateral jump shot, and the lowest in the step-back jump shot. Furthermore, the experimental group experienced a substantial reduction in sports injury rates, with the injury rate decreasing to 6%. These findings indicate that plyometric training not only enhances jumping performance, but also contributes to injury prevention by strengthening lower-limb muscles. This study provides a theoretical basis for coaches to develop comprehensive training programs that improve athletic performance and reduce injury risk.