Sporulation in Bacillus subtilis is independent of membrane fatty acid composition

Boudreaux, Don P.; Freese, Ernst

doi:10.1128/jb.148.2.480-486.1981

Cited by 5 publications

(1 citation statement)

References 19 publications

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“…These results show that the amount of anteiso-fatty acids can be increased by growing spores in the presence of L-isoleucine. Changes in spore fatty acid composition have also been reported for B. subtilis (32) and B. megaterium (12) when spores were grown on either ~-keto acid or amino acid precursors of fatty acids. In both cases, the investigators observed that the ability of vegetative cells to form spores was unaffected by the added fatty acid precursors.…”

Section: Fatty Acid Composition Of Vegetative Cells and Spores Grown mentioning

confidence: 61%

Fatty acid and phospholipid composition ofBacillus megaterium spores with altered germination properties

Vary

1982

Lipids

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The ability of spores to trigger germination was altered by growing spores at either a suboptimal temperature or in a rich medium modified by substituting L-isoleucine for D-glucose. Compared to the control, spores grown in the presence of isoleucine germinated more readily between 20 and 28 C, while spores grown at 20 C germinated slower than the control at any temperature tested. Analysis of the composition of these spores indicated that spores grown in the presence of isoleucine had much higher levels of anteiso-C15 fatty acids than the control, while the phospholipid composition and the phospholipid to protein ratio were unchanged. The fatty acid composition for spores grown at 20 C was comparable to that of the control spores, but the levels of diphosphatidylglycerol and phosphatidylglycerol were altered as well as the ratio of phospholipid to protein. Steady-state fluorescent anisotropy measurements were made with 1,6-diphenyl-1,3,5-hexatriene incorporated into membrane isolated from these spores. The membranes from spores grown in the presence of isoleucine were more "fluid" between 10 and 20 C than membranes from the control spores. Membranes from 20 C grown spores were less "fluid" between 10 and 38 C than membranes from the control spores. These results show that triggering of spore germination was altered by growing spores under conditions that altered the composition of spore membranes.

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Section: Fatty Acid Composition Of Vegetative Cells and Spores Grown mentioning

confidence: 61%

Fatty acid and phospholipid composition ofBacillus megaterium spores with altered germination properties

Vary

1982

Lipids

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The Bacillus Cell Envelope

Archibald

1989

Bacillus

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Induction of L-form-like cell shape change of Bacillus subtilis under microculture conditions

et al. 2003

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A remarkable cell shape change was observed in Bacillus subtilis strain 168 under microculture conditions on CI agar medium (Spizizen's minimal medium supplemented with a trace amount of yeast extract and Casamino acids). Cells cultured under a cover glass changed in form from rod-shaped to spherical, large and irregular shapes that closely resembled L-form cells. The cell shape change was observed only with CI medium, not with Spizizen's minimum medium alone or other rich media. The whole-cell protein profile of cells grown under cover glass and cells grown on CI agar plates differed in several respects. Tandem mass analysis of nine gel bands which differed in protein expression between the two conditions showed that proteins related to nitrate respiration and fermentation were expressed in the shape-changed cells grown under cover glass. The cell shape change of CI cultures was repressed when excess KNO 3 was added to the medium. Whole-cell protein analysis of the normal rod-shaped cells grown with 0?1 % KNO 3 and the shape-changed cells grown without KNO 3 revealed that the expression of the branched-chain a-keto acid dehydrogenase complex (coded by the bfmB gene locus) was elevated in the shape-changed cells. Inactivation of the bfmB locus resulted in the repression of cell shape change, and cells in which bfmB expression was induced by IPTG did show changes in shape. Transmission electron microscopy of ultrathin sections demonstrated that the shape-changed cells had thin walls, and plasmolysis of cells fixed with a solution including 0?1 M sucrose was observed. Clarifying the mechanism of thinning of the cell wall may lead to the development of a new type of cell wall biosynthetic inhibitor.

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Sporulation in Bacillus subtilis is independent of membrane fatty acid composition

Cited by 5 publications

References 19 publications

Fatty acid and phospholipid composition ofBacillus megaterium spores with altered germination properties

Fatty acid and phospholipid composition ofBacillus megaterium spores with altered germination properties

The Bacillus Cell Envelope

Induction of L-form-like cell shape change of Bacillus subtilis under microculture conditions

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