Sporulation ofBeauveria bassianaon Cadavers ofTriatoma infestansafter Infection at Different Temperatures and Doses of Inoculum

Selection of Beauveria bassiana isolates to control Alphitobius diaperinus

“…On the other hand, Rohde et al (2006) used a suspension containing 1.0 · 10 9 conidia/mL and observed a conidial production on the same insect of 0.02-3.0 · 10 7 conidia/insect. Some studies have shown that higher conidial productions are achieved when smaller amounts of the fungus are used in the inoculation suspension (Luz et al, 1999;Tarefa and Pringle, 2003). However, other studies have shown that conidial production on the body of dead insects increases as the conidial concentration in the suspension increases (Coutinho and Oliveira, 1991;Neves and Hirose, 2005).…”

Storage of Beauveria bassiana Conidia Suspension: A Study Exploring the Potential Effects on Conidial iability and Virulence against Dermanyssus gallinae De Geer, 1778 Acari: Dermanyssidae

“…Five dozen glass beads 6 mm in diameter were used to ensure complete homogenization. Turbidity was adjusted spectrophotometrically Biosan DEN 1 to an optical density of 7.5 McFarland, corresponding to 1-5 × 10 7 conidia/ml [6,7,16]. CIS was divided into 50 mL tubes Falcon ® Centrifuge Tubes, (Aptaca, Asti, Italy) and the concentration of conidia was calculated as reported before and adjusted in order to obtain the same concentration in each replicate at the start of the experiments T 0 .…”

Density-dependence and within-host competition in a semelparous parasite of leaf-cutting ants

“…This is even without taking into account the probability of at least one spore encountering a host, which will also be substantially increased by spores dispersing (and thus having multiple chances to encounter a host per unit time), rather than staying in a single aggregation (and having only a single chance per unit time to encounter a host). In other studies of obligate killer parasites, even stronger effects of within-host competition between propagules of the same parasite clone have been found [7,60]. It therefore seems likely that these dynamics may be broadly similar for most semelparous, obligate killer parasites, and that maximum dispersal is the best strategy for these parasites, as well as any others that exhibit strong within-host competition.…”

“…Although this competition was between genetically identical parasites in the first experiment, it still resulted in a reduction in parasite fitness because of the less efficient use of host resources. Were spore production to be unaffected by, or even inversely related to dose, as found in a study with Beauveria [60], then this negative effect on fitness would be even greater.…”