Spotlight on biodiversity of microbial cell factories for glycerol conversion

Rußmayer, Hannes; Egermeier, Michael; Kalemasi, Denis; Sauer, Michael

doi:10.1016/j.biotechadv.2019.05.001

Cited by 35 publications

(17 citation statements)

References 152 publications

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“…In order to develop a control strategy that enables the control of biomass concentration of extremely halophilic H. mediterranei, a continuous membrane-based cell retention system was used. A feedforward biomass control strategy was successfully developed on synthetic medium using glycerol as a cheap and widely available carbon source [30][31][32]. Furthermore, a biomass online monitoring tool was established to calculate biomass concentrations in steady state experiments.…”

Section: Development Of a Biomass Control And Monitoring Concept On Smentioning

confidence: 99%

Soft Sensor-Based Monitoring and Efficient Control Strategies of Biomass Concentration for Continuous Cultures of Haloferax mediterranei and Their Application to an Industrial Production Chain

et al. 2019

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Continuous bioprocessing using cell retention allows the achievement of high space-time yields for slow-growing organisms such as halophiles. However, the lack of efficient methods for monitoring and control limits the application of biotechnological processes in the industry. The aim of this study was to implement a control and online monitoring strategy for biomass in continuous cultures. For the first time, a feedforward cultivation strategy in a membrane-based cell retention system allowed to control the biomass concentration of the extreme halophilic Haloferax mediterranei at defined levels. Moreover, soft sensor-based biomass estimation allowed reliable monitoring of biomass online. Application of the combined monitoring and control strategy using industrial process water containing formate, phenol, aniline and 4,4′-methylenedianiline could for the first time demonstrate high throughput degradation in this extremophilic bioremediation process, obtaining degradation efficiencies of up to 100%. This process demonstrates the usefulness of continuous halophilic cultures in a circular economy application.

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Section: Development Of a Biomass Control And Monitoring Concept On Smentioning

confidence: 99%

Soft Sensor-Based Monitoring and Efficient Control Strategies of Biomass Concentration for Continuous Cultures of Haloferax mediterranei and Their Application to an Industrial Production Chain

et al. 2019

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“…This subclass is tolerant to oxygen. The enzyme inactivated by an excess of glycerol includes in the community of species Lactobacillus , Clostridium , Klebsiella , and Citrobacter [ 44 ].…”

Section: Metabolic Pathway Of Glycerolmentioning

confidence: 99%

“…Until now, only a single strain was shown to have glycerol dehydrates. However, genome analyses for another micro-organism that ferments glycerol showed that independent glycerol dehydratase contains vitamin B12 like C. difficile, C. botulinum , and other Clostridium species [ 44 ].…”

Section: Metabolic Pathway Of Glycerolmentioning

confidence: 99%

Glycerol waste to value added products and its potential applications

Chilakamarry

Sakinah

Zularisam

et al. 2021

Syst Microbiol and Biomanuf

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Graphic abstract The rapid industrial and economic development runs on fossil fuel and other energy sources. Limited oil reserves, environmental issues, and high transportation costs lead towards carbon unbiased renewable and sustainable fuel. Compared to other carbon-based fuels, biodiesel is attracted worldwide as a biofuel for the reduction of global dependence on fossil fuels and the greenhouse effect. During biodiesel production, approximately 10% of glycerol is formed in the transesterification process in a biodiesel plant. The ditching of crude glycerol is important as it contains salt, free fatty acids, and methanol that cause contamination of soil and creates environmental challenges for researchers. However, the excessive cost of crude glycerol refining and market capacity encourage the biodiesel industries for developing a new idea for utilising and produced extra sources of income and treat biodiesel waste. This review focuses on the significance of crude glycerol in the value-added utilisation and conversion to bioethanol by a fermentation process and describes the opportunities of glycerol in various applications.

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“…Glycerol is released as a by-product in the biodiesel industry with production of approximate ten percent of biodiesel in weight [11]. In particular, crude glycerol is an abundant and versatile carbon source for biochemicals production owing to its massive dropped price [12,13].…”

Section: Introductionmentioning

confidence: 99%

Quantitative proteomic analysis to reveal expression differences for butanol production from glycerol and glucose by Clostridium sp. strain CT7

Jiang

et al. 2021

Microb Cell Fact

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Clostridium sp. strain CT7 is a new emerging microbial cell factory with high butanol production ratio owing to its non-traditional butanol fermentation mode with uncoupled acetone and 1,3-propanediol formation. Significant changes of metabolic products profile were shown in glycerol- and glucose-fed strain CT7, especially higher butanol and lower volatile fatty acids (VFAs) production occurred from glycerol-fed one. However, the mechanism of this interesting phenomenon was still unclear. To better elaborate the bacterial response towards glycerol and glucose, the quantitative proteomic analysis through iTRAQ strategy was performed to reveal the regulated proteomic expression levels under different substrates. Proteomics data showed that proteomic expression levels related with carbon metabolism and solvent generation under glycerol media were highly increased. In addition, the up-regulation of hydrogenases, ferredoxins and electron-transferring proteins may attribute to the internal redox balance, while the earlier triggered sporulation response in glycerol-fed media may be associated with the higher butanol production. This study will pave the way for metabolic engineering of other industrial microorganisms to obtain efficient butanol production from glycerol.

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Spotlight on biodiversity of microbial cell factories for glycerol conversion

Cited by 35 publications

References 152 publications

Soft Sensor-Based Monitoring and Efficient Control Strategies of Biomass Concentration for Continuous Cultures of Haloferax mediterranei and Their Application to an Industrial Production Chain

Soft Sensor-Based Monitoring and Efficient Control Strategies of Biomass Concentration for Continuous Cultures of Haloferax mediterranei and Their Application to an Industrial Production Chain

Glycerol waste to value added products and its potential applications

Quantitative proteomic analysis to reveal expression differences for butanol production from glycerol and glucose by Clostridium sp. strain CT7

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