Spotted Fever Group Rickettsiae in Ticks, Morocco

Sarih, M’hammed; Socolovschi, Cristina; Boudebouch, Najma; Hassar, Mohammed; Raoult, Didier; Parola, Philippe

doi:10.3201/eid1407.070096

Cited by 78 publications

(51 citation statements)

References 40 publications

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“…using a previously developed qPCR assay targeting a 109-bp fragment of a hypothetical protein as previously described [11]. If a positive result was obtained, PCR amplification and sequencing targeting the ompA gene were used as previously described [12]. A negative control (sterile water and DNA from a sterile biopsy specimen) and a positive control (DNA from R. montanensis) were included in each test.…”

Section: Molecular Methods For the Detection Of Rickettsia Speciesmentioning

confidence: 99%

Rickettsia sibirica mongolitimonae infection, Sri Lanka

Cordier

Tattevin

Leyer

et al. 2017

J Infect Dev Ctries

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Introduction. Rickettsia sibirica mongolitimonae was recently reported as a common rickettsiosis in France. Current serological evidence suggests the presence of scrub typhus and spotted fever group rickettsiosis in Sri Lanka. We detected a human case of R. sibirica mongolitimonae in Sri Lanka. Methodology. A skin biopsy of the eschar was tested for the presence of Rickettsia spp. using qPCR assay targeting a 109-bp fragment of a hypothetical protein and by PCR amplification and sequencing targeting the ompA gene. Results. A 30-year-old woman who had just returned from travel to a jungle in Sri Lanka was evaluated as an outpatient for fever. Examination revealed an enlarged axillary lymph node, a maculopapular rash and an eschar at her left flank and a skin biopsy of the eschar was performed. The skin biopsy was positive for the presence of Rickettsia spp. by qPCR and PCR amplification and sequencing targeting the ompA gene revealed R. sibirica mongolitimonae. Immunofluorescence assay on an acute serum sample for spotted fever group rickettsial antigens (Rickettsia conorii conorii, R. sibirica mongolitimonae, Rickettsia felis) and typhus group rickettsiae (Rickettsia typhi) was negative. The patient was treated by oral doxycycline (200 mg/day) for one week. Conclusions. R. sibirica mongolitimonae should be considered in the differential diagnosis of patients with suspected rickettsiosis in, or returning from, Sri Lanka.

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Section: Molecular Methods For the Detection Of Rickettsia Speciesmentioning

confidence: 99%

Rickettsia sibirica mongolitimonae infection, Sri Lanka

Cordier

Tattevin

Leyer

et al. 2017

J Infect Dev Ctries

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“…7 The distribution of R. massiliae and its association with different Rhipicephalus spp. was subsequently extended to other Mediterranean and African countries, [8][9][10][11][12][13][14][15][16][17] and to Argentina. 18 The reported prevalence of R. massiliae in ticks has varied from 4.7% to 18%.…”

Section: Introductionmentioning

confidence: 99%

“…18 The reported prevalence of R. massiliae in ticks has varied from 4.7% to 18%. 11,12,15 However, the true prevalence and range of distribution of R. massiliae in Rhipicephalus ticks are unknown, especially in the New World. Whether the specific genetic types of R. massiliae have adapted preferentially to different tick vectors, animal hosts, and whether their potential to cause febrile illness varies are unknown.…”

Section: Introductionmentioning

confidence: 99%

A Focus of Dogs and Rickettsia massiliae–Infected Rhipicephalus sanguineus in California

Beeler¹,

Abramowicz²,

Zambrano³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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Abstract. A recurrent focus of Rhipicephalus sanguineus infestation was investigated in a suburban area of southern California after reports of suspected Rocky Mountain spotted fever in two dogs on the same property. Abundant quantities of Rh. sanguineus were collected on the property and repeatedly from each dog, and Rickettsia massiliae DNA was detected by polymerase chain reaction (PCR). Whole blood and serum samples from four dogs were tested by using PCR and microimmunofluorescent assay for antibodies against spotted fever group rickettsiae. Serum samples from all four dogs contained antibodies reactive with R. massiliae , R. rhipicephali , R. rickettsii , and 364D Rickettsia but no rickettsial DNA was detected by PCR of blood samples. Serum cross-absorption and Western blot assays implicated R. massiliae as the most likely spotted fever group rickettsiae responsible for seropositivity. To our knowledge, this is the first detection of R. massiliae in ticks in California.

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“…marginatum ticks tested ranges from 1.8% to 64% in different studies. 23,[27][28][29] Rickettsia aeschlimannii has also been detected in Hyalomma aegyptum (L.) in Algeria, 30 Haemaphysalis inermis Birula in Spain, 27 Hyalomma marginatum rufipes Koch in Egypt, Ethiopia, and Chad, 28,31 Hyalomma anatolicum excavatum Koch from the Greek Island of Cephalonia, 32 and Hyalomma dromedarii Koch and Hyalomma impeltatum Schulze and Schlottke from Egypt. 31 Furthermore, five other human biting tick species including Haemaphysalis punctata Canestrini and Fanzago, Ixodes ricinus (L.), Rh.…”

Section: Discussionmentioning

confidence: 99%

“…Rickettsia aeschlimannii was first described from H. marginatum marginatum ticks from Morocco in 1997 23 and was subsequently detected in 2002 in a patient returning from Morocco. 24 Subsequently, the presence of R. aeschlimannii has been demonstrated in H. marginatum marginatum ticks with a distribution from Portugal and northern Spain to Kazakhstan and from Mediterranean countries to South Africa.…”

Section: Discussionmentioning

confidence: 99%

Spotted Fever Group Rickettsiae in Ticks Collected from Wild Animals in Israel

Keysary¹,

Eremeeva²,

Leitner³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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Abstract. We report molecular evidence for the presence of spotted fever group rickettsiae (SFGR) in ticks collected from roe deer, addax, red foxes, and wild boars in Israel. Rickettsia aeschlimannii was detected in Hyalomma marginatum and Hyalomma detritum while Rickettsia massiliae was present in Rhipicephalus turanicus ticks. Furthermore, a novel uncultured SFGR was detected in Haemaphysalis adleri and Haemaphysalis parva ticks from golden jackals. The pathogenicity of the novel SFGR for humans is unknown; however, the presence of multiple SFGR agents should be considered when serological surveillance data from Israel are interpreted because of significant antigenic cross-reactivity among Rickettsia . The epidemiology and ecology of SFGR in Israel appear to be more complicated than was previously believed.

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Spotted Fever Group Rickettsiae in Ticks, Morocco

Abstract: Identified rickettsiae were 4 pathogens, 2 suspected pathogens, and 1 incompletely described species.

Cited by 78 publications

References 40 publications

Rickettsia sibirica mongolitimonae infection, Sri Lanka

Rickettsia sibirica mongolitimonae infection, Sri Lanka

A Focus of Dogs and Rickettsia massiliae–Infected Rhipicephalus sanguineus in California

Spotted Fever Group Rickettsiae in Ticks Collected from Wild Animals in Israel

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