SPR based immunosensor for detection of Legionella pneumophila in water samples

Enrico, De Lorenzis; Manera, Maria Grazia; Montagna, G.; Cimaglia, Fabio; Chiesa, Maurizio; Poltronieri, Palmiro; Santino, Angelo; Rella, R.

doi:10.1016/j.optcom.2012.12.064

Cited by 40 publications

(12 citation statements)

References 24 publications

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“…These assays can have various formats such as lateral flow assay (Helbig et al, 2006), enzyme-linked immunosorbent assay (ELISA) (Reveiller et al, 2003), immunochromatograpic test (Helbig et al, 2006), and can be combined with other detection methods (e.g., microscopy (Baba et al, 2012), cytometry (Fuechslin et al, 2010), sensors (Bekir et al, 2015; Enrico et al, 2013)). For instance, anti- L. pneumophila and anti- Legionella antibodies have been widely used in a number of immunoassays to detect L. pneumophila of various serogroups and/or distinguish L. pneumophila from other Legionella species (Delgado-Viscogliosi et al, 2005; Fuechslin et al, 2010).…”

Section: Detection Methodsmentioning

confidence: 99%

“…These techniques have recently been applied for live Legionella monitoring in drinking water samples (Delgado-Viscogliosi et al, 2005; Keserue et al, 2012). Other reported immunoanalytical methods include construction of miniaturized immonobiosensors (e.g., optic biosensor, impedimetric biosensor) with immobilized antibodies to provide rapid screening and on-site measurement of L. pneumophila and P. aeruginosa in contaminated water samples (Bekir et al, 2015; Enrico et al, 2013). …”

Section: Detection Methodsmentioning

confidence: 99%

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Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review

et al. 2017

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Opportunistic pathogens inhabiting premise (i.e., building) plumbing (OPPPs, e.g., L. pneumophila, M. avium complex, P. aeruginosa, Acanthamoeba, and N. fowleri) are a significant and growing source of disease. Because OPPPs establish and grow as part of the native drinking water microbiota, they do not correspond to fecal indicators, presenting a significant challenge to common and effective monitoring strategies. Further, different OPPPs present distinct requirements for sampling, preservation, and analysis, creating a significant impediment to their parallel detection. The aim of this critical review is to synthesize the state of the science of monitoring OPPPs and to identify a path forward for their simultaneous detection and quantification in a manner commensurate with the need for reliable data to inform risk assessment and mitigation. Water and biofilm sampling procedures, as well as factors influencing sample representativeness and detection sensitivity, are critically evaluated with respect to the five representative bacterial and amoebal OPPPs noted above. Available culturing and molecular approaches are discussed in terms of their advantages, limitations, and applicability. Knowledge gaps and research needs are identified.

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Section: Detection Methodsmentioning

confidence: 99%

Section: Detection Methodsmentioning

confidence: 99%

Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review

et al. 2017

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“…[12][13][14] Biosensors use biologically sensitive bioreceptors together with suitable transducers. 15 Among techniques explored for detection of L. pneumophila and Escherichia coli, the most popular are surface plasmon resonance, 16 surface acoustic wave, 17 and electrochemical impedance spectroscopy. 18 Significant attention has been paid toward the development of transducers since they could determine the overall performance and detection limit of a biosensor.…”

Section: Introductionmentioning

confidence: 99%

Photonic biosensor based on photocorrosion of GaAs/AlGaAs quantum heterostructures for detection of Legionella pneumophila

et al. 2016

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Photocorrosion of semiconductors is strongly sensitive to the presence of surface states, and it could be influenced by electrically charged molecules immobilized near the semiconductor/electrolyte interface. The underlying mechanism is related to band bending of the semiconductor structure near the surface and the associated distribution of excited electrons and holes. The authors have employed photoluminescence of GaAs/AlGaAs quantum heterostructures for monitoring in situ the photocorrosion effect, and demonstrating detection of nongrowing Legionella pneumophila suspended in phosphate buffered saline solution. Antibody functionalized samples allowed direct detection of these bacteria at 10(4) bacteria/ml. The authors discuss the sensitivity of the process related to the ability of creating conditions suitable for photocorrosion proceeding at extremely slow rates and the interaction of an electric charge of bacteria with the surface of a biofunctionalized semiconductor.

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“…The presence of pathogens in environmental matrices, and mainly in water compartments, could constitute a serious danger for human health and some biosensors were recently proposed for their environmental monitoring. For example, rapid and specific optical biosensors based on surface plasmon resonance were proposed for the detection of metabolically active Legionella pneumophila in complex environmental water samples [ 40 , 41 ]. In one work, the principle of detection was based on the recognition of bacterial RNA by the RNA detector probe immobilized on the biochip gold surface [ 40 ].…”

Section: Biosensors For Environmental Monitoringmentioning

confidence: 99%

“…Streptavidin-conjugated quantum dots were used for signal amplification and the detection time was about three hours, suggesting the viability of the biosensing system for effective detection of bacteria in a range of 10 4 –10 8 CFU mL −1 [ 40 ]. In the second work, the gold substrate was functionalized with a self-assembled monolayer of protein A and an antibody solution against L. pneumophila [ 41 ]. A limit of detection of 10 3 CFU mL −1 was obtained and the biosensor was able to detect L. pneumophila in contaminated water samples in 30 min since any labelling was needed and the immobilization of antibodies in biosensor surface by protein A in a highly oriented manner was stable and effective [ 41 ].…”

Section: Biosensors For Environmental Monitoringmentioning

confidence: 99%

Recent Progress in Biosensors for Environmental Monitoring: A Review

Justino

Duarte

Rocha-Santos

2017

Sensors

306

125

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The environmental monitoring has been one of the priorities at the European and global scale due to the close relationship between the environmental pollution and the human health/socioeconomic development. In this field, the biosensors have been widely employed as cost-effective, fast, in situ, and real-time analytical techniques. The need of portable, rapid, and smart biosensing devices explains the recent development of biosensors with new transduction materials, obtained from nanotechnology, and for multiplexed pollutant detection, involving multidisciplinary experts. This review article provides an update on recent progress in biosensors for the monitoring of air, water, and soil pollutants in real conditions such as pesticides, potentially toxic elements, and small organic molecules including toxins and endocrine disrupting chemicals.

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SPR based immunosensor for detection of Legionella pneumophila in water samples

Cited by 40 publications

References 24 publications

Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review

Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review

Photonic biosensor based on photocorrosion of GaAs/AlGaAs quantum heterostructures for detection of Legionella pneumophila

Recent Progress in Biosensors for Environmental Monitoring: A Review

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