SPR biosensor for the detection of L. monocytogenes using phage-displayed antibody

Nanduri, Viswaprakash; Bhunia, Arun K.; Tu, Shu‐I; Paoli, George C.; Brewster, Jeffrey D.

doi:10.1016/j.bios.2007.04.007

Cited by 135 publications

(67 citation statements)

References 30 publications

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“…This emphasized that charge-directed immobilization might be helpful to improve the infectivity of less virulent phages. Physical adsorption has been used successfully to immobilize IG40 and Lm P4:A8 filamentous phages on gold surfaces of a quartz crystal microbalance (QCM) biosensor and a surface plasmon resonance (SPR) sensor surface to detect E. coli and L. monocytogenes, respectively (25,26). Another filamentous phage specific to Salmonella Typhimurium has been physically adsorbed to the surface of a magnetoelastic sensor and used as a biorecognition element for the detection of its host cells at different concentrations (20).…”

Section: Discussionmentioning

confidence: 99%

“…The potential use of a phage-based biosorbent to detect, concentrate, and identify target bacteria has been reported in several studies (10,42). In one approach, physical adsorption has been used recently to immobilize filamentous and Podoviridae phages on gold surfaces of surface plasmon resonance (SPR) sensors and glass substrates to be used as a recognition element for many targeted pathogens (2,13,26). In an earlier study, Salmonella cells were captured from food matrices using Salmonella-specific phage passively immobilized on polystyrene, but this resulted in a low capture efficiency (3).…”

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confidence: 99%

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Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in Meat by Using Phages Immobilized on Modified Cellulose Membranes

Anany

Chen

Pelton

et al. 2011

Appl Environ Microbiol

202

140

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The ability of phages to specifically interact with and lyse their host bacteria makes them ideal antibacterial agents. The range of applications of bacteriophage can be extended by their immobilization on inert surfaces. A novel method for the oriented immobilization of bacteriophage has been developed. The method was based on charge differences between the bacteriophage head, which exhibits an overall net negative charge, and the tail fibers, which possess an overall net positive charge. Hence, the head would be more likely to attach to positively charged surfaces, leaving the tails free to capture and lyse bacteria. Cellulose membranes modified so that they had a positive surface charge were used as the support for phage immobilization. It was established that the number of infective phages immobilized on the positively charged cellulose membranes was significantly higher than that on unmodified membranes. Cocktails of phages active against Listeria or Escherichia coli immobilized on these membranes were shown to effectively control the growth of L. monocytogenes and E. coli O157:H7 in ready-to-eat and raw meat, respectively, under different storage temperatures and packaging conditions. The phage storage stability was investigated to further extend their industrial applications. It was shown that lyophilization can be used as a phage-drying method to maintain their infectivity on the newly developed bioactive materials. In conclusion, utilizing the charge difference between phage heads and tails provided a simple technique for oriented immobilization applicable to a wide range of phages and allowed the retention of infectivity.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in Meat by Using Phages Immobilized on Modified Cellulose Membranes

Anany

Chen

Pelton

et al. 2011

Appl Environ Microbiol

202

140

View full text Add to dashboard Cite

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“…This fusion protein self-assembled onto solid surface with the nanobody pointing outwards from the protein lattice surface into the solution. A similar approach was used to fuse scFvs with pIII surface protein, allowing a more sensitive detection to be achieved [33]. Fusion of Ab fragments with proteins such as beta-galactosidase, maltose-binding protein, calmodulin-binding protein, chitin-binding domain, cellulose-binding domain or glutathione-S-transferase is also possible.…”

Section: Antibody-binding Proteinsmentioning

confidence: 99%

Trends and Perspectives in Immunosensors

2012

Antibodies Applications and New Developments

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Immunosensors are devices that comprise both a biomolecular recognition system, such as an antibody and its corresponding antigen, and a transducer to translate the high affinity and specific binding event into a physical signal.Antibodies are produced by an immunological response to the presence of a foreign substance called an antigen. Antibodies may be immobilised onto a variety of platforms including bulk planar surfaces and nanoparticles by either covalent or adsorption strategies. Different interfaces between the bio-components and the detector are available to monitor in 'real-time' the signal generated by biological interactions. The transducers detect, for example, the change in electron transfer, absorbance, fluorescence, refractive index, mass change or heat transfer as the antibody binds to the antigen of interest. Such analytical devices have allowed a wide range of analytes to be identified and quantified such as pathogens, toxins, environmental food contaminants and disease biomarkers.The demand for sensitive, rapid, 'on-site' techniques has taken advantage of the latest advances in microfluidics and nanotechnology. This chapter will highlight current trends in immobilisation, micro/nano-fluidics/ and transducers utilised. A number of examples outlining the exploitation of these elements in immunosensors and their successful applications will be described.

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“…B. von BIAcore) haben sich als nützlich für die Untersuchung von Biomolekül-Wechselwirkungen in Echtzeit erwiesen. [113,127] Phagen können bei der Entwicklung von spezifischen SPR-Sensoren in zweierlei Weise genutzt werden: [110,114,113,[127][128][129] durch Verankern des zielspezifischen Phagen auf einem Sensorchip [110,127] sowie bei der Selektion eines zielspezifischen Proteins mithilfe eines Phagendisplays und anschließendem Verankern dieses Proteins auf dem Sensorchip. [113,114] Durch Einführung eines zielspezifischen Phagen in einen SPR-Sensor konnte dessen Leistungsfähigkeit merklich gesteigert werden.…”

Section: Oberflächenplasmonenresonanz-sensorenunclassified

Die Anwendung von Viren in Chemo‐ und Biosensoren

2009

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Viren haben sich in jüngster Zeit als einzigartige Hilfsmittel für den empfindlichen und selektiven Nachweis von Analyten wie Explosivstoffen, Proteinen, Bakterien, Viren, Sporen und Toxinen erwiesen. Auf Bakterien spezialisierte Viren, die Bakteriophagen – oder kurz Phagen –, wurden dabei am intensivsten untersucht: Zielspezifische nichtlysierende Phagen (sowie von diesen präsentierte Peptide und Proteine) können mithilfe hoch entwickelter Phagendisplaytechniken identifiziert werden, und lysierende Phagen können gezielt Bakterien aufbrechen und spezifische Zellmarker wie Enzyme ausschütten, die analysiert werden können. Darüber hinaus sind Phagen chemisch wie thermisch hinreichend stabil, und sie lassen sich mit Molekülen kuppeln und mit Nanomaterialien kombinieren und auf der Oberfläche eines Signalwandlers in einem Analysesystems verankern. Im Mittelpunkt dieses Aufsatzes stehen Fortschritte bei der Verwendung von Phagen in Chemo‐ und Biosensoren durch Kombination mit etablierten analytischen Techniken. Überdies werden aktuelle Entwicklungen bei der Verwendung von Virus‐Nanomaterial‐Kompositen und anderen Viren in Sensoranwendungen vorgestellt.

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SPR biosensor for the detection of L. monocytogenes using phage-displayed antibody

Cited by 135 publications

References 30 publications

Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in Meat by Using Phages Immobilized on Modified Cellulose Membranes

Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in Meat by Using Phages Immobilized on Modified Cellulose Membranes

Trends and Perspectives in Immunosensors

Die Anwendung von Viren in Chemo‐ und Biosensoren

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