Spray drying as a strategy for biosurfactant recovery, concentration and storage

Barcelos, Gisely Souza; Dias, Lívia Cristina Pinto; Fernandes, Péricles Leonardo; Fernandes, Rita de Cássi R; Borges, Arnaldo Chaer; Kalks, Karlos Hm; Tótola, Marcos Rogério

doi:10.1186/2193-1801-3-49

Cited by 10 publications

(8 citation statements)

References 24 publications

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“…This result enables us to confirm that the structures of these LPPs are preserved after a short-time exposure at high temperatures (up to 143°C). This observation is in line with other previous studies that have demonstrated that the surfactant properties of surfactin for instance are preserved after a short-time exposure at temperatures of up to 170°C ( Barcelos et al, 2014 ). Then, these different commercial LPPs in powder form have also been resuspended in demineralized water and subsequently spray-dried.…”

Section: Resultssupporting

confidence: 93%

“…1-7.3]. This confirms the results obtained by Barcelos et al (2014), who showed that the addition of maltodextrin at 10% is effective for the spraydrying of supernatant of B. subtilis-containing LPPs. Nevertheless, we demonstrated that 4 times less maltodextrin is sufficient as filler to make the spray-drying of these supernatant-containing LPPs easier.…”

Section: The Specific Case Of Mycosubtilinsupporting

confidence: 88%

“…Iturin A produced by B. subtilis was also spray-dried to be incorporated into microcapsules, with sodium alginate and poly(γ-glutamic acid) as wall materials, to improve the compound stability and facilitate its storage ( Yu et al, 2017 ). The addition of a high amount of maltodextrin or kaolinite as drying adjuvants also enabled the spray-drying of surfactin without any loss in surfactant activity ( Barcelos et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

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Impact of the Purification Process on the Spray-Drying Performances of the Three Families of Lipopeptide Biosurfactant Produced by Bacillus subtilis

Vassaux

Rannou

Peers³

et al. 2021

Front. Bioeng. Biotechnol.

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Lipopeptides produced by Bacillus subtilis display many activities (surfactant, antimicrobial, and antitumoral), which make them interesting compounds with a wide range of applications. During the past years, several processes have been developed to enable their production and purification with suitable yield and purity. The already implemented processes mainly end with a critical drying step, which is currently achieved by freeze-drying. In this study, the possibility to replace this freeze-drying step with a spray-drying one, more suited to industrial applications, was analyzed. After evaluating their thermal resistance, we have developed a spray-drying methodology applicable for the three lipopeptides families produced by B. subtilis, i.e., surfactin, mycosubtilin (iturin family), and plipastatin (fengycin family). For each lipopeptide, the spray-drying procedure was applied at three steps of the purification process by ultrafiltration (supernatant, diafiltered solution, and pre-purified fraction). The analysis of the activities of each spray-dried lipopeptide showed that this drying method is not decreasing its antimicrobial and biosurfactant properties. The methodology developed in this study enabled for the first time the spray-drying of surfactin, without adjuvants’ addition and regardless of the purification step considered. In the case of fengycin and mycosubtilin, only diafiltered solution and purified fraction could be successfully spray-dried without the addition of adjuvant. Maltodextrin addition was also investigated as the solution for the direct drying of supernatant. As expected, the performances of the spray-drying step and the purity of the powder obtained are highly related to the purification step at which the product was dried. Interestingly, the impact of mycosubtilin concentration on spray-drying yield was also evidenced.

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Section: Resultssupporting

confidence: 93%

Section: The Specific Case Of Mycosubtilinsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Impact of the Purification Process on the Spray-Drying Performances of the Three Families of Lipopeptide Biosurfactant Produced by Bacillus subtilis

Vassaux

Rannou

Peers³

et al. 2021

Front. Bioeng. Biotechnol.

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“…Some studies describe the use of spray drying for the conservation of biosurfactants and later application (Saeki et al, 2009 ). Spray drying has also been effective in the recovery and concentration of a biosurfactants while maintaining their surface activity and the dry product maintained its characteristics and activity during storage at room temperature throughout a 120 day evaluation period (Barcelos et al, 2014 ).…”

Section: Resultsmentioning

confidence: 99%

Candida lipolytica UCP0988 Biosurfactant: Potential as a Bioremediation Agent and in Formulating a Commercial Related Product

et al. 2017

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The aim of the present study was to investigate the potential application of the biosurfactant from Candida lipolytica grown in low-cost substrates, which has previously been produced and characterized under optimized conditions as an adjunct material to enhance the remediation processes of hydrophobic pollutants and heavy metals generated by the oil industry and propose the formulation of a safe and stable remediation agent. In tests carried out with seawater, the crude biosurfactant demonstrated 80% oil spreading efficiency. The dispersion rate was 50% for the biosurfactant at a concentration twice that of the CMC. The biosurfactant removed 70% of motor oil from contaminated cotton cloth in detergency tests. The crude biosurfactant also removed 30-40% of Cu and Pb from standard sand, while the isolated biosurfactant removed ∼30% of the heavy metals. The conductivity of solutions containing Cd and Pb was sharply reduced after biosurfactants' addition. A product was prepared through adding 0.2% potassium sorbate as preservative and tested over 120 days. The formulated biosurfactant was analyzed for emulsification and surface tension under different pH values, temperatures, and salt concentrations and tested for toxicity against the fish Poecilia vivipara. The results showed that the formulation had no toxicity and did not cause significant changes in the tensoactive capacity of the biomolecule while maintaining activity demonstrating suitability for potential future commercial product formulation.

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“…The glucose units are primarily linked with a (1 ? 4) glycosidic bonds (Barcelos et al 2014). In the present study, the presence of maltodextrin was determined in term of total sugar using phenol-sulfuric acid method.…”

Section: Total Sugar Contentsmentioning

confidence: 96%

Effect of maltodextrin on characteristics and antioxidative activity of spray-dried powder of gelatin and gelatin hydrolysate from scales of spotted golden goatfish

Chuaychan

Benjakul

2016

J Food Sci Technol

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Characteristics and antioxidative activity of gelatin and gelatin hydrolysate powders from scale of spotted golden goatfish using maltodextrin as a carrier agent at different ratios [1:0, 2:1, 1:1 and 1:2 (w/w)] were investigated. Gelatin hydrolysates with 40 % degree of hydrolysis exhibited the highest antioxidative activity. With increasing maltodextrin proportions, the resulting powders showed an increase in yields, total sugar content and whiteness with coincidental decrease in [Formula: see text], [Formula: see text]-values and browning intensity. Solubility of gelatin powder increased with increase in maltodextrin proportion. Gelatin powder was spherical with smooth surface of hydrolysate varied, regardless of maltodextrin levels. Gelatin hydrolysate powder form, uniform agglomerates when maltodextrin was incorporated. DPPH and ABTS radical scavenging activities and ferric-reducing antioxidant power of gelatin and gelatin hydrolysate decreased when maltodextrin was used as a carrier agent. Thus, maltodextrin levels directly affected characteristics and antioxidative activity of gelatin and gelatin hydrolysate powders.

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Spray drying as a strategy for biosurfactant recovery, concentration and storage

Cited by 10 publications

References 24 publications

Impact of the Purification Process on the Spray-Drying Performances of the Three Families of Lipopeptide Biosurfactant Produced by Bacillus subtilis

Impact of the Purification Process on the Spray-Drying Performances of the Three Families of Lipopeptide Biosurfactant Produced by Bacillus subtilis

Candida lipolytica UCP0988 Biosurfactant: Potential as a Bioremediation Agent and in Formulating a Commercial Related Product

Effect of maltodextrin on characteristics and antioxidative activity of spray-dried powder of gelatin and gelatin hydrolysate from scales of spotted golden goatfish

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