Spray-drying performance of a bench-top spray dryer for protein aerosol powder preparation

Abstract

The objective of this work was to improve a bench‐top spray dryer's efficiency in both production recovery and throughput for preparing protein aerosol powders. A Büchi mini‐spray dryer was used to prepare the powders of recombinant humanized anti‐IgE antibody. The resulting powder's physical properties such as particle size, residual moisture, and morphology, along with its recovery and production rate was the basis of this development work. Mass balance suggests that approximately 10–20% of powder was lost in the exhaust air, consisting primarily of particles less than 2 μm. Also, significant loss (20–30%) occurred in the cyclone. Attempts were made to improve product recovery in the receiving vessel using dual‐cyclone configurations, different cyclone designs, cyclones with anti‐static treatment, and different receiver designs. System modifications such as replacing the original bag‐filter unit with a vacuum system effectively reduced drying air flow resistance, allowing the protein to be dried at a lower inlet air temperature and the production scale to be increased. We concluded that the modified spray‐drying system is advantageous over the original bench‐top spray dryer. This improvement will be beneficial to early‐stage research and development involving high‐valued protein powders. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 301–309, 1998.

Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes

“…Moreover, downstream processing may benefit from ‘state of the art’ tomato food/beverage processing technology. For instance, liquid spry‐drying, a process increasingly used in antibody formulation (Maa et al. , 1998), is also employed in the food industry for the production of tomato powder (Santos de Sousa et al.…”

Application of cyclodextrins in antibody microparticles: potentials for antibody protection in spray drying

“…The harvesting yield is normally between 30-40% in protein dry powder preparation using bench top spray dryer (28). It mainly depends on the special cyclone design of the spry drying apparatus (28).…”

Preparation, in-vitro and in-vivo evaluation of spray-dried ternary solid dispersion of biopharmaceutics classification system class II model drug

“…The low yield for solid dispersion may be acceptable for a small-scale spray dryer, since the cyclone separator is unable to trap particles <2 μm, which sticks to the inner wall of the drying chamber. [27,29] An increase in the polymer ratio enhanced the per cent yield of TSD (Figure 1a). This is due to heightened molecular interaction between the drug and polymer or with increased polymer concentration; the rate of core to surface transport of solvent decreases with an increase in the feed viscosity due to higher fraction or molecular weight of polymer present in solution.…”