Spread of invasive Spanish Staphylococcus aureus spa-type t067 associated with a high prevalence of the aminoglycoside-modifying enzyme gene ant(4')-Ia and the efflux pump genes msrA/msrB

Pérez-Vázquez, Marı́a; Vindel, Ana; Marcos, Carmen; Oteo, Jesús; Cuevas, Óscar; Trincado, Pilar; Bautista, Verónica; Grundmann, Hajo; Campos, José

doi:10.1093/jac/dkn430

Cited by 59 publications

(48 citation statements)

References 56 publications

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“…The distribution of SCCmec types among MRSA isolates indicated that SCCmec type III was the prominent type (71.4%) in the current survey. This pattern is comparable to the distribution of SCCmec types among MRSA reported in China (37) and Brazil (30), but differs from that reported by Vazquez et al in Spain (38). This SCCmec type was previously reported as the most prevalent type in Iran by Japoni et al In the present study, the gene coding for pvl was found only among MRSA strains that harbored SCCmec type IV.…”

Section: Discussionsupporting

confidence: 83%

Distribution of SCCmec Types in Methicillin-Resistant Staphylococcus aureus Isolated from Burn Patients

Afshari

Sepahi

Goudarzi

et al. 2017

Arch Clin Infect Dis

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Background: Methicillin resistance Staphylococcus aureus (MRSA) isolates have been recognized as one of the most important causes of infection in burn patients that have recently shown a frequent and rapid development of antibiotic resistance. Objectives: The present study described the distribution of different SCCmec types and antibiotic resistance pattern among MRSA strains isolated from burn patients in a referral burn center in Tehran, Iran. Methods: During a 12-month study, 189 MRSA isolates were obtained from burn patients. Standard culture and biochemical tests were used for identification of MRSA isolates. The antibiotic susceptibility testing was performed using disc diffusion method and the presence of mecA, nucA, pvl, and tst encoding genes was determined using PCR method. In addition, the different SCCmec types were determined using multiplex PCR. Results: All the MRSA isolates were observed to be resistant to amoxicillin and penicillin and sensitive to linezolid, teicoplanin, and vancomycin. The rates of resistance to other antibiotics varied from 86.2% for amikacin to 17.5% for quinupristin-dalfopristin. MDR was observed in all the isolates. Six different antimicrobial resistance patterns were observed among our isolates. Based on the multiplex PCR assay, the two different SCCmec types were detected as 71.4% type III and 28.6% type IV. In total, 8.5% of isolates harbored pvl toxin encoding gene all of which belonging to SCCmec type IV. Furthermore, 33 isolates (17.5%) harbored tst encoding gene. Conclusions:The results showed low diversity of SCCmec type among circulating MRSA in the burn center with relatively high prevalence of SCCmec III. These findings support the need for more studies to elucidate distribution of different SCCmec types among MRSA isolated from burn patients.

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Section: Discussionsupporting

confidence: 83%

Distribution of SCCmec Types in Methicillin-Resistant Staphylococcus aureus Isolated from Burn Patients

Afshari

Sepahi

Goudarzi

et al. 2017

Arch Clin Infect Dis

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“…8 Currently, the most widespread HA-MRSA clone is ST125/SCCmecIV/t067 which is an allelic variant of ST5 pediatric clone. 9 MRSA population dynamics is undergoing significant changes due to demographic variations such as immigration and the development of sophisticated and complex health systems (i.e. care of patients with severe underlying diseases and invasive devices in day hospitals, residency in a long-term care facility.…”

Section: Q2mentioning

confidence: 99%

Antimicrobial resistance, virulence factors and genetic lineages of hospital-onset methicillin-resistant Staphylococcus aureus isolates detected in a hospital in Zaragoza

González-Domínguez

Seral

Potel

et al. 2015

Enfermedades Infecciosas y Microbiología Clínica

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Please cite this article in press as: González-Domínguez M, et al. b s t r a c tIntroduction: MRSA population dynamics is undergoing significant changes, and for this reason it is important to know which clones are circulating in our nosocomial environment. Materials and methods: A total of 118 MRSA isolates were collected from clinical samples from patients with previous hospital or healthcare contact (named as hospital-onset MRSA (HO-MRSA)) during a one year period. Susceptibility testing was performed by disk diffusion and microdilution. The presence of resistance genes and virulence factors were tested by PCR. All isolates were typed by SCCmec, spa and agr typing. PFGE and MLST were applied to a selection of them.Results: Eighty-three HO-MRSA isolates (70.3%) were resistant to any antibiotic included in the macrolide-lincosamide-streptogramin B group. Among these isolates, the M phenotype was the most frequent (73.5%). One hundred and seven of HO-MRSA isolates (90.7%) showed aminoglycoside resistance. The combination aac(6 )-Ie-aph(2 )-Ia + ant (4 ) El CC5 fue el más prevalente (88,1%) seguido de CC8 (5,9%), CC22 (2,5%), CC398 (2,5%) y CC1 (0,8%). Conclusión: La línea clonal CC5/ST125/t067 fue la más habitual. Esta línea se relacionó con resistencia a aminoglucósidos, y, en menor medida, con macrólidos. La presencia de clones internacionales como EMRSA-15 (CC22/ST22), clones europeos como CC5/ST228, clones comunitarios relacionados con CC1 o CC8 y clones asociados al ganado, como el CC398, se observaron en un bajo porcentaje. ARTICLE IN PRESSG Model EIMC 1282 1-7 2 M. González-Domínguez et al. / Enferm Infecc Microbiol Clin. 2015;xxx(xx):xxx-xxx

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“…Staphylococcus aureus are Gram positive bacteria that cause diseases such as skin and soft tissues infections as well as food poisoning and toxic shocks [24]. The rate of mortality associated with Staphylococcus aureus in developing world exceeds the one of developed countries [25].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Potency of Methanolic Leaf Extracts from Selected Medicinal Plants against Staphylococcus aureus

Ho¹,

Pe²,

Wm³

et al. 2016

J Med Microb Diagn

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The main aim of the study was to test for the antimicrobial potency of Aloe secundiflora, Bulbine frutescens, Tagetes minuta and Vernonia lasiopus against Staphylococcus aureus. All the plants showed a pronounced antimicrobial activity against Staphylococcus aureus with Tagetes minuta being the most active at low concentrations (MIC 8.9 mg/ml; MBC 10.0 mg/ml) whereas Vernonia lasiopus showing less activity (MIC 12.2 mg/ml; MBC 14.2 mg/ml). The efficacy test was carried out using the disc diffusion method. The standard antibiotics used were ciprofloxacin (5 µg/ml) and vancomycin (3 µg/ml) showed significant antimicrobial activity by producing zones of inhibition of 22 mm and 25 mm respectively. Dimethyl sulphoxide and distilled water were used as negative control. The extracts from the plants were also screened for the presence of phytochemicals with the results showing the presence of flavonoids, alkaloids, tannins and saponins in all the extracts. The study suggested that the selected medicinal plants can be used effectively in the treatment of bacterial infection caused by Staphylococcus aureus.

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Spread of invasive Spanish Staphylococcus aureus spa-type t067 associated with a high prevalence of the aminoglycoside-modifying enzyme gene ant(4')-Ia and the efflux pump genes msrA/msrB

Abstract: A higher than expected prevalence of spa-type t067 isolates was found among invasive MRSA in Spain. The oxacillin, tobramycin, erythromycin and ciprofloxacin resistance profile of spa-type t067 isolates was linked to the presence of ant(4')-Ia and msrA or msrB genes.

Cited by 59 publications

References 56 publications

Distribution of SCCmec Types in Methicillin-Resistant Staphylococcus aureus Isolated from Burn Patients

Distribution of SCCmec Types in Methicillin-Resistant Staphylococcus aureus Isolated from Burn Patients

Antimicrobial resistance, virulence factors and genetic lineages of hospital-onset methicillin-resistant Staphylococcus aureus isolates detected in a hospital in Zaragoza

Antimicrobial Potency of Methanolic Leaf Extracts from Selected Medicinal Plants against Staphylococcus aureus

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