In this study, novel wound dressing materials based on non-woven (NW) surfaces were developed using the electrospraying (e-spraying) process. Polyester spun-bond (PET SPB), polypropylene spun-bond (PP SPB), and polypropylene melt-blown (PP MB) surfaces used as matrix, and folic acid (FA), vitamin B9weresprayed on these surfaces. The resulting NW fabrics with the same FA content were investigated in terms of physical, morphological, thermal, wettability properties.Scanning Electron Microscopy and (SEM) and Fourier Transform Infrared (FT-IR) spectroscopy results showed the formation of physical interaction between NWs and FA.Notably, FA was successfully deposited onto NWs with average fiber diameters from 2.6 µm to 23.11 µm. According to the thermogravimetric analysis (TGA),FA loaded-PP SPB has enhanced thermal stability compared to pure one (PP SPB). The FA-loaded surfaces have a hydrophobic property with contact angles values more than >90°. The in-vitro release was carried out by UV-Vis within the 8 hour-period phosphate buffer saline (pH 7.2). The results indicated that FA-loaded surfaces have a fast release behaviour. The total FA release amounts of the FA-loaded PET SBP, PP SPB and PP MB NWs were found as 22.8, 17.1, and 17.5 ppm. Moreover, the biocompatibility of all resulting NW surfaces was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red uptake (NRU) cytotoxicity tests in L929 cell lines.The obtained NWs are biocompatible and non-toxic material, except PET SPB-sFA.The study indicated that FA-loaded NWs can be potential candidates for wound healing applications.