Spurious Automated Platelet Count Enumeration of Yeast Forms as Platelets by the Cell-DYN 4000

Latif, Shahnila; Veillon, Diana M.; Brown, Donald J.; Kaltenbach, Jenny; Curry, Sherry; Linscott, Andrea J.; Oberle, Arnold; Cotelingam, James D.

doi:10.1309/h2k6-e59l-yfe6-3gpb

Cited by 14 publications

(16 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, platelet clumps and giant platelets owing to their large size are not counted as platelets in automated analyzers. All these lead to inaccurate platelet counts in an automated hematology analyzer [8][9][10][11] . Hence platelet count values obtained from automated analyzers should be crosschecked by some other method particularly in cases of thrombocytopenia [12] .…”

Section: Discussionmentioning

confidence: 99%

A novel method to estimate platelet counts from peripheral smears: A study comparing a new method of platelet estimation with existing methods.

Sudalaimuthu¹,

Ganapathy²,

Rajendran³

et al. 2017

APALM

View full text Add to dashboard Cite

Background: Automated hematology analyzers have a low degree of accuracy in predicting platelet counts in patients with thrombocytopenia. Hence automated platelet counts should be crosschecked by reviewing the peripheral blood smear in cases of thrombocytopenia. However existing methods for platelet count estimation from peripheral blood smears have certain drawbacks. Hence we suggest a new method to estimate platelet count from peripheral smear and compared it with the existing methods. Conclusion:Platelet count estimation based on platelet/RBC ratio in ten oil immersion fields and total RBC count is a simple and reliable method to estimate platelet counts from peripheral smears.

show abstract

Section: Discussionmentioning

confidence: 99%

A novel method to estimate platelet counts from peripheral smears: A study comparing a new method of platelet estimation with existing methods.

Sudalaimuthu¹,

Ganapathy²,

Rajendran³

et al. 2017

APALM

View full text Add to dashboard Cite

show abstract

“…Fungemia might be suspected also from spurious increase of white blood cell count or platelet count from a complete blood count [11]. These findings are explained by the fact that automated blood analyzers may misidentify Candida spp.…”

Section: Discussionmentioning

confidence: 99%

Heroin with a Twist: Candida Glabrata Endocarditis Concurrent with Brain Mycetomas in an IV Drug User

Panos¹

2017

JMEN

View full text Add to dashboard Cite

A 47-year-old male, with a history of IV drug use and usage of fresh lemon juice in the preparation of heroin doses, with hepatitis B and hepatitis C, presented with fever, dyspnea and a new found cardiac murmur at the apex. Echocardiography showed large vegetations on both leaflets of the mitral valve. After blood cultures were drawn an empiric antibiotic regimen was initiated. The mitral valve was successfully replaced surgically. Twenty-four hours post surgically the patient was extubated and was found to be suffering from left hemiplegia. Brain CT scan showed diffused sites of hemorrhage of the brain parenchyma, compatible with septic emboli that had underwent a hemorrhagic conversion. As mycetomas were considered in the differential diagnosis of these lesions, new blood cultures were drawn with an emphasis on the isolation of fungi, as this correlates with the use of lemon juice in the preparation of heroin doses, while the treatment regimen was modified to include an echinocandin to cover extensively for Candida spp. Two sets of blood cultures were positive for Candida glabrata. The patient responded well to the treatment administered and regained a significant amount of mobility.

show abstract

“…It is also well known that yeast is an interference factor of blood cell count in automated hematology systems. Arnold et al [7] and Latif et al [8] reported that C. glabrata fungemia can lead to a falsely elevated platelet count on the H*2 (Bayer HealthCare, Diagnostics Division, Tarrytown, NY, USA) and on the Cell-DYN 4000 (Abbott Diagnostics, Santa Clara, CA, USA) hematology analyzers in thrombocytopenic patients.…”

Section: Discussionmentioning

confidence: 99%

“…The most common isolate is C. albicans followed by C. parapsilosis and C. glabrata. Latif et al [8] revealed that C. glabrata, which has a mean volume of 12 fL, can be confused with a large platelet in an automated blood analyzer that uses an optical scatter or electrical impedence method to detect the blood cells. Large size yeasts such as C. albicans or C. parapsilosis do not show similarity with platelets [8].…”

Section: Discussionmentioning

confidence: 99%

“…Latif et al [8] revealed that C. glabrata, which has a mean volume of 12 fL, can be confused with a large platelet in an automated blood analyzer that uses an optical scatter or electrical impedence method to detect the blood cells. Large size yeasts such as C. albicans or C. parapsilosis do not show similarity with platelets [8]. Therefore, candidemia by C. parapsilosis would not cause any numerical changes in platelet counts in ADVIA but just result in abnormal flag, because yeasts clumped together and also resided within WBCs ( Fig.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Misidentification ofCandida parapsilosisas Large Platelets in an Automated Blood Analyzer

Kim

Ryoo

et al. 2009

Korean J Clin Microbiol

View full text Add to dashboard Cite

show abstract

Spurious Automated Platelet Count Enumeration of Yeast Forms as Platelets by the Cell-DYN 4000

Cited by 14 publications

References 10 publications

A novel method to estimate platelet counts from peripheral smears: A study comparing a new method of platelet estimation with existing methods.

A novel method to estimate platelet counts from peripheral smears: A study comparing a new method of platelet estimation with existing methods.

Heroin with a Twist: Candida Glabrata Endocarditis Concurrent with Brain Mycetomas in an IV Drug User

Misidentification ofCandida parapsilosisas Large Platelets in an Automated Blood Analyzer

Contact Info

Product

Resources

About