Sputum Phagocytes from Healthy Individuals Are Functional and Activated: A Flow Cytometric Comparison with Cells in Bronchoalveolar Lavage and Peripheral Blood

Polymorphonuclear leucocytes consume oxygen in sputum from chronic Pseudomonas aeruginosa pneumonia in cystic fibrosis

“…PMNs in bronchoalveolar lavage fluids from chronically infected patients with CF and in sputum from healthy individuals have the potential to mount a respiratory burst upon stimulation 14 15. In contrast, the presence of PMNs with an active respiratory burst was demonstrated in fresh sputum from patients with CF with chronic P aeruginosa lung infection in this study.…”

Inflammation in induced sputum after aluminium oxide exposure: an experimental chamber study

“…Flow cytometry (FACS calibur, Becton Dickinson (BD), Oxford, England) was used for differential cell counts according to the procedure described by Alexis et al 20 with some modifications. Gating of leucocytes in sputum was determined by a combination of light-scatter characteristics and positive/negative expression for relevant surface markers, that is, lymphocytes CD5+/CD45+, macrophages HLA-DR+/CD16+/CD45+, neutrophils HLA-DR-/CD16+/CD45+, and eosinophils CD5+/CD16−/CD45+ (HLA-DR, CD5[Diatec, Oslo, Norway], CD16[Serotec, Oxford, England], and CD45[BD]).…”

Recovery from workplace-induced airway inflammation 1 year after cessation of exposure

“…Quantities of monoclonal antibodies were determined with a titration test. Comparison of newly recruited neutrophils and older neutrophils was done according to Alexis et al 11 …”