Sputum processing methods to improve the sensitivity of smear microscopy for tuberculosis: a systematic review

Steingart, Karen R; Ng, Vivienne; Henry, Marie‐Claire; Hopewell, Philip C.; Ramsay, Andrew; Cunningham, Jane; Urbanczik, R; Perkins, Mark D.; Aziz, Mohamed Abdel; Pai, Madhukar

doi:10.1016/s1473-3099(06)70602-8

Cited by 483 publications

(375 citation statements)

References 39 publications

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“…Especialmente em países de baixa e média renda, o diagnóstico se baseia na baciloscopia, devido às reduzidas exigências de infraestrutura para a sua realização e ao custo reduzido 1 . No entanto, a acurácia do teste é baixa 4 , o que contribui para o atraso no diagnóstico e, por consequência, no aumento da mortalidade e morbidade, e na perpetuação da cadeia de transmissão 5 .…”

Section: Introductionunclassified

Impacto orçamentário da incorporação do GeneXpert MTB/RIF para o diagnóstico da tuberculose pulmonar na perspectiva do Sistema Único de Saúde, Brasil, 2013-2017

et al. 2017

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Resumo: O objetivo do estudo foi estimar o impacto orçamentário do GeneXpert MTB/RIF para o diagnóstico da tuberculose sob a perspectiva do Programa Nacional de Controle da Tuberculose, valendo-se de um modelo estático apoiado no método epidemiológico entre 2013 e 2017. Comparou-se um teste Xpert MTB/RIF com duas baciloscopias diagnósticas. Utilizaram-se dados epidemiológicos, populacionais, de custos, a taxa de câmbio e bases de dados do Sistema Único de Saúde. Foi realizada análise de sensibilidade por cenários. A incorporação do GeneXpert MTB/RIF demandaria um montante de R$ 147 milhões em cinco anos e representaria um impacto de 23% a 26% nos dois primeiros anos, e de cerca de 11% entre 2015 e 2017. Os resultados podem apoiar os gestores brasileiros e dos países latino-americanos no planejamento e gestão na sua decisão de incorporação da tecnologia.

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Section: Introductionunclassified

Impacto orçamentário da incorporação do GeneXpert MTB/RIF para o diagnóstico da tuberculose pulmonar na perspectiva do Sistema Único de Saúde, Brasil, 2013-2017

et al. 2017

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“…La baciloscopia continúa siendo internacionalmente la herramienta primaria en el diagnóstico de la TBC pulmonar activa; esta es la prueba más utilizada no sólo en la búsqueda de casos infecciosos de la comunidad, sino además como medidor de la eficacia del tratamiento en estos pacientes 2,3 .…”

Section: Introductionunclassified

Importancia del control de la calidad de la baciloscopia en los laboratorios de diagnóstico de tuberculosis

Sardiñas

García

Martinez

et al. 2016

Rev. chil. infectol.

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Importance of quality control of baciloscopy in laboratories that perform diagnosis of tuberculosisBackground: Baciloscopy is the primary tool for pulmonary tuberculosis diagnosis, being this technique the most used internationally in the search for infectious cases. Quality control is the process of the rechecking smears by a highly qualified observer. Aim: To evaluate and highlight the importance of quality control of smear microscopy in the Provincial Laboratories diagnosticians of Tuberculosis in Cuba. Methods: This study was conducted at the National Reference Laboratory and Research in Tuberculosis, Leprosy and Mycobacteria in the Institute of Tropical Medicine "Pedro Kouri", Havana, Cuba, Were evaluated 2676 smears received from January 2013 to December 2014, from Provincial Centers of Hygiene, Epidemiology and Microbiology of Cuba, including the special municipality Isla de la Juventud. Results: 2,664 (99.5%) were concordant smears, the correlation obtained for the positive smears were 96.5% and 99.8% for negative. Were identified12 reading errors: 7 (3.5%) false positive and 5 (0.2%) false negatives. Slides were classified with adequate quality of smears in 2039 (76.2%), showed difficulties in realizing the extension in 1464 (54.7%) and staining were adequate in 2343 (87.6%). The kappa index was 0.9674. Conclusion: Although there was good agreement between observations it is recommended to improve the quality of extended, maintain staff training program that performs this activity, like regular supervision by specialists, to further improve the quality of diagnosis.

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“…Traditional diagnosis of pulmonary tuberculosis depends on sputum smear, which is rapid, specific and inexpensive but has low sensitivity ranging from 20 % to 60 %, when performed optimally [3], whereas sputum culture is timeconsuming and chest imaging examination is with unsatisfactory result. SNPT is more difficult to diagnose and has been associated with poorer treatment outcomes and excessive mortality, particularly in high human immunodeficiency virus prevalent settings.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic value of nucleic acid amplification tests on bronchoalveolar lavage fluid for smear-negative pulmonary tuberculosis: a meta-analysis

et al. 2015

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SynopsisThe diagnosis of smear-negative pulmonary tuberculosis (SNPT) remains a clinical challenge. Many studies suggest that nucleic acid amplification tests (NAATs) on bronchoalveolar lavage fluid (BALF) plays a role in diagnosing SNPT, but with considerable varying results. The current study aimed to summarize the overall diagnostic accuracy of NAATs assay on BALF for SNPT. A systematic literature search was performed and data were retrieved. Pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR) and diagnostic odds ratio (DOR) were calculated. A summary receiver operating characteristic curve and area under the curve (AUC) were used to evaluate the overall diagnostic performance. All the statistical analysis was performed by using STATA 12.0 and Meta-DiSc 1.4 software. A total of nine studies with 1214 subjects were included this meta-analysis. The pooled sensitivity, specificity, PLR, NLR and DOR were 0.

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Sputum processing methods to improve the sensitivity of smear microscopy for tuberculosis: a systematic review

Cited by 483 publications

References 39 publications

Impacto orçamentário da incorporação do GeneXpert MTB/RIF para o diagnóstico da tuberculose pulmonar na perspectiva do Sistema Único de Saúde, Brasil, 2013-2017

Impacto orçamentário da incorporação do GeneXpert MTB/RIF para o diagnóstico da tuberculose pulmonar na perspectiva do Sistema Único de Saúde, Brasil, 2013-2017

Importancia del control de la calidad de la baciloscopia en los laboratorios de diagnóstico de tuberculosis

Diagnostic value of nucleic acid amplification tests on bronchoalveolar lavage fluid for smear-negative pulmonary tuberculosis: a meta-analysis

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