SpWRKY1 mediates resistance to Phytophthora infestans and tolerance to salt and drought stress by modulating reactive oxygen species homeostasis and expression of defense-related genes in tomato

Li, Jing-bin; Luan, Yushi; Liu, Zhen

doi:10.1007/s11240-015-0815-2

Cited by 82 publications

(68 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In plant–pathogen interactions, ROS are rapidly produced following infection, which may inhibit pathogen growth by preventing pathogen from entering the cell or inducing resistant genes (Bradley et al ., ; Kotchoni and Gachomo, ; Wi et al ., ). However, later generation of massive amounts of ROS is toxic to the cell and may lead to cell membrane damage, enhance susceptibility and cause apoptosis (Li et al ., ). In plants, the ROS scavenging system that regulates the steady‐state level of ROS plays important roles to prevent oxidative damage.…”

Section: Discussionmentioning

confidence: 97%

“…(Kotchoni and Gachomo, ). In our previous work, we showed that overexpression of SpWRKY1 in tomato regulates antioxidants to reduce ROS accumulation and alleviate cell membrane injury after P. infestans infection (Li et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Among all S. pimpinellifolium accessions, one line ( L3708 ) has been identified to be highly resistant to a wide range of P. infestans isolates (Chunwongse et al ., ; Nowakowska et al ., ; Zhang et al ., ). Another cultivated tomato, S. lycopersicum Zaofen No.2 , is a susceptible accession to a variety of pathogens including P. infestans (Wang et al ., , ; Gao et al ., ; Guo et al ., ; Li et al ., ). In our previous work, SpWRKY1 in L3708 was identified, which was induced by P. infestans .…”

Section: Introductionmentioning

confidence: 97%

“…Compared with tomato Zaofen No. 2 , SpWRKY1 ‐ overexpressed tomato markedly enhanced resistance to P. infestans (Li et al ., ).…”

Section: Introductionmentioning

confidence: 97%

See 3 more Smart Citations

Comparative transcriptome analysis between resistant and susceptible tomato allows the identification of lncRNA16397 conferring resistance to Phytophthora infestans by co‐expressing glutaredoxin

et al. 2017

View full text Add to dashboard Cite

The rapid development of omics sequencing technology has facilitated the identification of thousands of long non-coding (lnc)RNAs in plant species, but the role of lncRNAs in plant-pathogen interactions remains largely unexplored. We used comparative transcriptome analysis of Phytophthora infestans-resistant and -susceptible tomatoes to identify differentially expressed genes (DEGs) and lncRNAs (DELs), and examine lncRNA-mRNA networks. A total of 1037 DEGs and 688 DELs were identified between P. infestans-resistant and -susceptible tomatoes. The co-localization networks, including 128 DEGs and 127 DELs, were performed. We found that lncRNA16397 acted as an antisense transcript of SlGRX22 to regulate its expression, and also induced SlGRX21 expression when lncRNA16397 was overexpressed. In addition, disease symptoms and reactive oxygen species (ROS) accumulation in tomatoes overexpressing lncRNA16397 and SpGRX were fewer and lower than those in wild-type after P. infestans infection. This result suggests that tomato lncRNA16397 induces SlGRX expression to reduce ROS accumulation and alleviate cell membrane injury, resulting in enhanced resistance to P. infestans. Our results provide insight into lncRNAs involved in the response of tomato to P. infestans infection, demonstrate that the lncRNA16397-GRXs network is an important component of the P. infestans network in tomato, and provide candidates for breeding to enhance biotic stress-resistance in tomato.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

“…Compared with tomato Zaofen No. 2 , SpWRKY1 ‐ overexpressed tomato markedly enhanced resistance to P. infestans (Li et al ., ).…”

Section: Introductionmentioning

confidence: 97%

See 2 more Smart Citations

Comparative transcriptome analysis between resistant and susceptible tomato allows the identification of lncRNA16397 conferring resistance to Phytophthora infestans by co‐expressing glutaredoxin

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Its fungal disease is currently a major hurdle restricting the quality of soybean and causes damage to the crop's yield (Li, Luan, & Liu, 2015). However, the complex physiology of pathogenic bacteria makes it difficult to breed a resistant plant to break through the problem (Dorrance, Jia, & Abney, 2004;Kim, et al, 2013), and conventional breeding methods have limitations which make it difficult to fulfill the production needs (Molinar, 2012).…”

Section: Introductionmentioning

confidence: 99%

Genetic Stability and Disease Resistance Analysis of Hrpzpsta Gene in Transgenic Soybean Lines

Yu¹,

Wang²,

Song³

et al. 2017

JPS

View full text Add to dashboard Cite

This experiment was carried out to evaluate genetic stability and disease resistance in transformed soybean lines with hrpZpsta gene using PCR analysis, southern blotting, real-time quantitative PCR (qRT-PCR) and to analyze the resistance against Phytophthora sojae (P. sojae) and Cercospora sojina (C. sojina) after inoculation. The results obtained using PCR and southern blotting analytical methods showed that exogenous gene functional elements were stably inherited in transgenic soybean and hrpZpsta gene was successfully integrated into the soybean genome in a single copy. Results at high-generation (T 7 , T 8 ) transgenic lines of hrpZpsta revealed that their relative expression of hrpZpsta gene was the highest in leaves followed by roots, and much lower in stems, flowers, and seeds. Activity change rates of peroxidase (POD), polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) showed that transgenic lines significantly enhanced receptor species. The resistance of transgenic strains T 7 and T 8 generations against P. sojae was significantly increased with artificial inoculation methods, and the resistance against C. sojina was increased from susceptibility to the level of resistance. Under natural conditions in the field, the response of T 8 transgenic lines to C. sojina reached disease resistance level. There were no significant differences in transgenic lines and recipient variety in maturing stage, leaf shape, flower color, plant height, 100-grain weight and quality content, and the two years average yield of plots increased to 11.59% and 8.19%, which significantly higher than recipient cultivar. The current results provide data support for the release of transgenic lines.

show abstract

The Role of Transcription Factors in Response to Biotic Stresses in Tomato

Jena

Sahu²,

Pradhan³

et al. 2022

Transcription Factors for Biotic Stress Tolerance in Plants

View full text Add to dashboard Cite

SpWRKY1 mediates resistance to Phytophthora infestans and tolerance to salt and drought stress by modulating reactive oxygen species homeostasis and expression of defense-related genes in tomato

Cited by 82 publications

References 77 publications

Comparative transcriptome analysis between resistant and susceptible tomato allows the identification of lncRNA16397 conferring resistance to Phytophthora infestans by co‐expressing glutaredoxin

Comparative transcriptome analysis between resistant and susceptible tomato allows the identification of lncRNA16397 conferring resistance to Phytophthora infestans by co‐expressing glutaredoxin

Genetic Stability and Disease Resistance Analysis of Hrpzpsta Gene in Transgenic Soybean Lines

The Role of Transcription Factors in Response to Biotic Stresses in Tomato

Contact Info

Product

Resources

About