Squalene Loaded Nanoparticles Effectively Protect Hepatic AML12 Cell Lines against Oxidative and Endoplasmic Reticulum Stress in a TXNDC5-Dependent Way

Bidooki, Seyed Hesamoddin; Alejo, Teresa; Sánchez-Marco, Javier; Martínez‐Beamonte, Roberto; Abuobeid, Roubi; Burillo, Juan Carlos; Lasheras, Roberto; Sebastián, Víctor; Rodríguez-Yoldi, María Jesús; Arruebo, Manuel; Osada, Jesús

doi:10.3390/antiox11030581

Cited by 18 publications

(12 citation statements)

References 86 publications

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“…It was the first time that squalene was found in the seminal vesicles of drones. It has been shown that squalene plays a significant role in many aspects, including anti-oxidation, prevention of cell mutation, and liver protection (Bidooki et al, 2022;Shafaq et al, 2022;Zhang et al, 2022). Thus, we speculate that squalene maintains sperm activity in the hypoxic environment of the seminal vesicles, which was demonstrated Frontiers in Physiology frontiersin.org in vitro semen storage experiments.…”

Section: Discussionsupporting

confidence: 53%

Spermatophore development in drones indicates the metabolite support for sperm storage in honey bees (Apis cerana)

et al. 2023

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Developing effective long-term sperm storage strategies to maintain activity requires an understanding of the underlying spermatophore developmental phase in drones. Here we compared the developmental processes and metabolites about seminal vesicles of drones from different parentages (0-24 d)in honeybee colonies, including mated queens, virgin queens, and worker bees. The results showed a similar developmental trend of seminal vesicles in thethree groups of drones on the whole, although there were significant differences in developmental levels, as well as in other indicators. Correlation analysis showed significant positive correlations between seminal vesicle width and sperm viability. The metabolomics of the seminal vesicles in drones from mated queens showed differences of the metabolites in each stage. Particularly, squalene identified among them was validated a protective effect on sperm vitality in vitro experiments. Together the results of these assays support that there were significant differences in the developmental levels of seminal vesicles among the three groups of drones in honeybees, wherein a significant correlation between sperm viability and the developmental levels of seminal vesicles were dissected. The metabolomics analysis and semen storage experiments in vitro display signatures of squalene that may act as an effective protective agent in maintaining sperm viability. Collectively, our findings indicate that spermatophore development in drones provides metabolite support, which contributes to research on the differences of sperm viability among drones in the future.

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Section: Discussionsupporting

confidence: 53%

Spermatophore development in drones indicates the metabolite support for sperm storage in honey bees (Apis cerana)

et al. 2023

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“…In addition, several other key proteins related to protein folding, such as XBP1, PDIA4, TXNDC5, DNAJC5B, and DNAJC5, also showed varying degrees of changes. TXNDC5 not only participates in protein folding but is also an essential antioxidant molecule [ 61 , 62 ]. The results suggested that these molecular chaperone-related genes were activated and important in protecting organisms against environmental stress.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of gene expression profiling from the deep sea in situ to the laboratory for the cold seep mussel Gigantidas haimaensis

Zhang

Yao

2022

BMC Genomics

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Background The deep-sea mussel Gigantidas haimaensis is a representative species from the Haima cold seep ecosystem in the South China Sea that establishes endosymbiosis with chemotrophic bacteria. During long-term evolution, G. haimaensis has adapted well to the local environment of cold seeps. Until now, adaptive mechanisms responding to environmental stresses have remained poorly understood. Results In this study, transcriptomic analysis was performed for muscle tissue of G. haimaensis in the in situ environment (MH) and laboratory environment for 0 h (M0), 3 h (M3) and 9 h (M9), and 187,368 transcript sequences and 22,924 annotated differentially expressed genes (DEGs) were generated. Based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, these DEGs were enriched with a broad spectrum of biological processes and pathways, including those associated with antioxidants, apoptosis, chaperones, immunity and metabolism. Among these significantly enriched pathways, protein processing in the endoplasmic reticulum and metabolism were the most affected metabolic pathways. These results may imply that G. haimaensis struggles to support the life response to environmental change by changing gene expression profiles. Conclusion The present study provides a better understanding of the biological responses and survival strategies of the mussel G. haimaensis from deep sea in situ to the laboratory environment.

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“…To enhance its delivery and improve its cellular uptake, squalene was encapsulated into PLGA nanoparticles. PLGA nanoparticles loaded with squalene protected mouse hepatocytes from oxidative and endoplasmic reticulum stresses by several molecular mechanisms [ 102 ]. However, the authors noticed some limitations in the PLGA encapsulation efficiency of squalene.…”

Section: Polyphenols From the Mediterranean Diet And Potential Carrie...mentioning

confidence: 99%

Enhancing the bioavailability and activity of natural antioxidants with nanobubbles and nanoparticles

Čolić,

Kraljević Pavelić,

Peršurić

et al. 2024

Redox Report

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Objectives: Many polyphenols such as EGCG from green tea, curcumin, apigenin, resveratrol or the alkaloid berberine show in-vitro activity that is much higher than FDA and EU approved drugs. And yet, while mice studies show excellent results, human clinical trials have so far been quite disappointing. Methods: In this review paper we accordingly present data from scientific literature and publicly available databases that support further development of nanoformulations for enhanced natural antioxidants bioavailability and activity. Results: Nanobubbles, and nanoparticles may enhance the polyphenols bioavailability and special coatings can be used to avoid liver fast inactivation. Zeolites have also been tested as carriers for bioactive compounds from the Mediterranean food. Other polyphenols can be used with nanoemulsions for synergistic antioxidants treatment. Finally, nanobubbles in the presence of ferric or copper ions at low pH as in human stomach or lysosomes can produce small amount of hydroxyl radicals, stimulating activation of Nrf2 transcription factor and detox enzymes. Discussion: A number of nanoformulations, including nanobubbles loaded with natural antioxidants may be used with low carbs and protein diet as a support to chemotherapy or treatment of advanced tumors. Good results in animal studies of neurodegenerative diseases and type II diabetes were also observed. KEY POLICY HIGHLIGHTS Nanobubbles and nanoparticles may enhance the polyphenols’ bioavailability Nanobubbles may stimulate the activation of Nrf2 and detox enzymes Armoured oxygen nanobubbles may enhance radiotherapy or chemotherapy effects

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Squalene Loaded Nanoparticles Effectively Protect Hepatic AML12 Cell Lines against Oxidative and Endoplasmic Reticulum Stress in a TXNDC5-Dependent Way

Cited by 18 publications

References 86 publications

Spermatophore development in drones indicates the metabolite support for sperm storage in honey bees (Apis cerana)

Spermatophore development in drones indicates the metabolite support for sperm storage in honey bees (Apis cerana)

Transcriptome analysis of gene expression profiling from the deep sea in situ to the laboratory for the cold seep mussel Gigantidas haimaensis

Enhancing the bioavailability and activity of natural antioxidants with nanobubbles and nanoparticles

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