Squalene Synthase Gene from Medicinal Herb Bacopa monniera: Molecular Characterization, Differential Expression, Comparative Modeling, and Docking Studies

Vishwakarma, Rishi K.; Patel, Kamlesh; Sonawane, Prashant; Kumari, Uma; Singh, Somesh; Ruby,; Abbassi, Shakeel; Agrawal, Dinesh Chandra; Tsay, Hsin‐Sheng; Khan, Bashir M.

doi:10.1007/s11105-015-0864-z

Cited by 19 publications

(10 citation statements)

References 55 publications

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“…BmSQS mRNA expression level was found to be higher in vegetative parts (roots) as compared to floral parts. Methyl jasmonate induces the BmSQS mRNA expression in all tissues tested, while salicylic acid, cold, and salt induce much higher expression in roots 97 . Comparative transcriptome analysis of shoot and root tissue of Bacopa monnieri identifies potential genes related to triterpenoid saponin biosynthesis.…”

Section: Analysis Of Metabolic Pathway Gene Expressionmentioning

confidence: 89%

Untitled

2019

IJPSR

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In recent times, the use of herbal products has increased tremendously in the developing as well as in developed countries. Bacopa monnieri is one of the most important medicinal plant that has been consumed for thousands of year as a memory enhancing, anti-inflammatory, analgesic, antipyretic, sedative and anti-epileptic agent. For many years, a lot of commercial and medicinal researches have been focusing their resources on this plant. The plant has been known to possess various activities such as, antibacterial, anti-fungal, anti-cancer, anti-oxidant, anti-inflammatory, antihyperglycemic, anti-depressant, anti-epileptic, memory enhancer, anti-ulcer, hepatoprotective, analgesic, anti-diarrheal, anti-hypertensive, anti-toxicity etc. Therefore step should be taken for its sustainable use and conservation. This review describes the phytoconstituents, traditional uses, and pharmacological activities of Bacopa monnieri. In brief, the biotechnological applications such as tissue culture, elicitation, genetic transformation for the advancement of this plant and its active components production have also been described. Habitat, Geographical Distribution, and Ethnomedical Description: Bacopa monnieri is a perennial, creeping herb whose habitat includes wetlands and muddy shores. The leaves are succulent, relatively thick, oblanceolate and are

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Section: Analysis Of Metabolic Pathway Gene Expressionmentioning

confidence: 89%

Untitled

2019

IJPSR

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“…Domains I, II, and III are involved in the first step of SS biosynthesis; domain IV participates in the second step; domain VI is a hydrophobic region that binds to the endoplasmic reticulum. The Mg 2+ -binding aspartate-rich regions and amino acids in the active site (Asp77, Asp81, Tyr168, Asp213, and Asp217) were highly conserved in SS amino acid sequences among different plant species [37][38][39]. Previously, it has been shown that overexpression of the SS (AB115496) gene significantly increases the ginsenoside content [40] in Panax ginseng.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of Clinopodium gracile (Benth.) Matsum and identification of genes related to Triterpenoid Saponin biosynthesis

et al. 2020

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Background: Clinopodium gracile (Benth.) Matsum (C. gracile) is an annual herb with pharmacological properties effective in the treatment of various diseases, including hepatic carcinoma. Triterpenoid saponins are crucial bioactive compounds in C. gracile. However, the molecular understanding of the triterpenoid saponin biosynthesis pathway remains unclear. Results: In this study, we performed RNA sequencing (RNA-Seq) analysis of the flowers, leaves, roots, and stems of C. gracile plants using the BGISEQ-500 platform. The assembly of transcripts from all four types of tissues generated 128,856 unigenes, of which 99,020 were mapped to several public databases for functional annotation. Differentially expressed genes (DEGs) were identified via the comparison of gene expression levels between leaves and other tissues (flowers, roots, and stems). Multiple genes encoding pivotal enzymes, such as squalene synthase (SS), or transcription factors (TFs) related to triterpenoid saponin biosynthesis were identified and further analyzed. The expression levels of unigenes encoding important enzymes were verified by quantitative real-time PCR (qRT-PCR). Different chemical constituents of triterpenoid saponins were identified by Ultra-Performance Liquid Chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS). Conclusions: Our results greatly extend the public transcriptome dataset of C. gracile and provide valuable information for the identification of candidate genes involved in the biosynthesis of triterpenoid saponins and other important secondary metabolites.

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“…The MVA pathway initiates with the acetyl-coenzyme A, which condenses into the acetoacetyl-CoA by the catalyzing action of acetyl-CoA acetyltransferase. Acetyl-coenzyme A is converted to isopentenyl diphosphate (IPP) through MVA, and HMGR (HMG-CoA reductase) which finally supplies carbon for the bacoside biosynthesis 17 18 . In the present investigation, both the microbes upregulated the expression of HMGR , a post-transcriptionally as well as post-translationally regulated gene responsible for catalyzing the conversion of HMG-CoA to mevalonate 19 .…”

Section: Discussionmentioning

confidence: 99%

Microbial modulation of bacoside A biosynthetic pathway and systemic defense mechanism in Bacopa monnieri under Meloidogyne incognita stress

Gupta

Singh

Srivastava

et al. 2017

Sci Rep

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Plant-associated beneficial microbes have been explored to fulfill the imperative function for plant health. However, their impact on the host secondary metabolite production and nematode disease management remains elusive. Our present work has shown that chitinolytic microbes viz., Chitiniphilus sp. MTN22 and Streptomyces sp. MTN14 singly as well as in combination modulated the biosynthetic pathway of bacoside A and systemic defense mechanism against Meloidogyne incognita in Bacopa monnieri. Interestingly, expression of bacoside biosynthetic pathway genes (3-Hydroxy-3-methylglutaryl coenzyme A reductase, mevalonate diphosphate decarboxylase, and squalene synthase) were upregulated in plants treated with the microbial combination in the presence as well as in absence of M. incognita stress. These microbes not only augmented bacoside A production (1.5 fold) but also strengthened host resistance via enhancement in chlorophyll a, defense enzymes and phenolic compounds like gallic acid, syringic acid, ferulic acid and cinnamic acid. Furthermore, elevated lignification and callose deposition in the microbial combination treated plants corroborate well with the above findings. Overall, the results provide novel insights into the underlying mechanisms of priming by beneficial microbes and underscore their capacity to trigger bacoside A production in B. monnieri under biotic stress.

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Squalene Synthase Gene from Medicinal Herb Bacopa monniera: Molecular Characterization, Differential Expression, Comparative Modeling, and Docking Studies

Cited by 19 publications

References 55 publications

Untitled

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Transcriptome analysis of Clinopodium gracile (Benth.) Matsum and identification of genes related to Triterpenoid Saponin biosynthesis

Microbial modulation of bacoside A biosynthetic pathway and systemic defense mechanism in Bacopa monnieri under Meloidogyne incognita stress

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