Squalestatin-induced production of taxol and baccatin in cell suspension culture of yew (Taxus baccata L.)

Amini, Sayed Asadollah; Shabani, Leila; Afghani, Ladan; Jalalpour, Zohreh; Sharifi-Tehrani, Majid

doi:10.3906/biy-1401-47

Cited by 16 publications

(6 citation statements)

References 37 publications

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“…Hence, we propose that subsequent retrograde redox signals, rather than chloroplast ROS per se , may act on MdMIR156 transcription. During phase change in apple, the contents of H 2 O 2 and GSH varied significantly within a considerable extent, in chemically-treated apple in vitro shoots, concentrations of H 2 O 2 and GSH changed drastically, even to an extreme level 10 , 65 , 66 , 67 . But in all cases, miR156 levels varied with GSH concentration and GSH/GSSG ratio but not directly with H 2 O 2 concentration 10 .…”

Section: Discussionmentioning

confidence: 99%

miR156 switches on vegetative phase change under the regulation of redox signals in apple seedlings

Jia

Chen

et al. 2017

Sci Rep

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In higher plants, miR156 regulates the vegetative phase change via the target SBP/SPL genes. The regulation of miR156 during ontogenetic processes is not fully understood. In the apple genome, of 31 putative MdMIR156 genes that encode pre-miR156, seven were dominantly expressed. However, the transcript levels of only MdMIR156a5 and MdMIR156a12 decreased significantly during the vegetative phase change, which was consistent with the mature miR156 level, indicating that miR156 is under transcriptional regulation. Leaf H2O2 content was higher in the adult phase than in the juvenile phase because of excess H2O2 accumulation in chloroplasts. When in vitro shoots were treated with menadione, diphenyleneiodonium, L-2-oxothiazolidine-4-carboxylic acid or buthionine sulphoximine, the expressions of MdMIR156a5, MdMIR156a12, and as well miR156 were coordinated with reduced glutathione (GSH) contents and glutathione/glutathione disulfide ratio but not H2O2 contents. Alteration of miR156 expression level by MdMIR156a6-overexpressing or miR156-mimetic transgenic Nicotiana benthamiana did not cause a corresponding change in reactive oxygen species or GSH status. Collectively, the results indicate that the vegetative phase change in apple is controlled by the MdMIR156a5 and MdMIR156a12 transcriptional regulatory network in response to the plastid–nucleus redox signals, such as GSH.

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Section: Discussionmentioning

confidence: 99%

miR156 switches on vegetative phase change under the regulation of redox signals in apple seedlings

Jia

Chen

et al. 2017

Sci Rep

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“…Isoprenoids and sterols were utilized as substrates for the accumulation of taxanes [20]. The increase in production of taxol or their intermediate baccatin III might be due to the effect of SQase on the gene expression at the initial step in the isoprenoid pathway [20]. These sterols are synthesized from mevalonic acid via the isoprenoid pathway [58].…”

Section: Resultsmentioning

confidence: 99%

“…Supplementation of sterol biosynthesis inhibitors such as tebuconazole and triadimefon in the culture medium showed increased yield of fungal taxol [19]. Amini et al [20] reported that squalestatin induced accumulation of H 2 O 2 and endogenous methyl jasmonate (MeJ) but suppressed squalene synthase (SQase) activity. These were correlated with the overexpression of key genes, resulting in remarkable increase in taxane production.…”

Section: Introductionmentioning

confidence: 99%

Salicylic acid as an effective elicitor for improved taxol production in endophytic fungus Pestalotiopsis microspora

et al. 2019

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Salicylic acid (SA) is an effective elicitor to increase taxol production in Pestalotiopsis microspora . Addition of SA at the concentration of 300 μM yielded taxol 625.47 μg L -1 , 45- fold higher than that of the control. Elicitation of the role of SA in the fungal taxol biosynthetic pathway revealed that SA enhanced reactive oxygen species and lipid peroxidation of unsaturated fatty acids of P . microspora mycelia. This oxidative process stimulates isoprene biosynthetic pathway by triggering expression of the geranylgeranyl pyrophosphate synthase gene leading to improved biosynthesis of taxol in P . microspora .

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“…As we all know, the most effective components of medical plants are secondary metabolites and elicitation of plant cells in culture represents a useful biotechnological tool to improve the production of these valuable metabolites. Therefore, the application of fungal elicitors largely solvesthe problem of low amounts of the active ingredients such as, the application of squalestatin significantly increasestaxol and baccatin III yields in cell suspension culture of yew (Taxus baccata L.) (Amini et al 2014). However, not all the fungal are useful and suited to the production of secondary metabolites, some pathogenic fungal cause cell hypersensitive responses and programed cell death, but non-pathogenic fungal may either cause general defense responses or no response.…”

Section: Discussionmentioning

confidence: 99%

The regulatory mechanism of fungal elicitor-induced secondary metabolite biosynthesis in medical plants

Zhai

Jia

Chen

et al. 2016

Critical Reviews in Microbiology

143

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A wide range of external stress stimuli trigger plant cells to undergo complex network of reactions that ultimately lead to the synthesis and accumulation of secondary metabolites. Accumulation of such metabolites often occurs in plants subjected to stresses including various elicitors or signal molecules. Throughout evolution, endophytic fungi, an important constituent in the environment of medicinal plants have known to form long-term stable and mutually beneficial symbiosis with medicinal plants. The endophytic fungal elicitor can rapidly and specifically induce the expression of specific genes in medicinal plants which can result in the activation of a series of specific secondary metabolic pathways resulting in the significant accumulation of active ingredients. Here we summarize the progress made on the mechanisms of fungal elicitor including elicitor signal recognition, signal transduction, gene expression and activation of the key enzymes and its application to this process. This paper provides guidance on studies which may be conducted to promote the efficient synthesis and accumulation of active ingredients by the endogenous fungal elicitor in medicinal plant cells, and provides new ideas and methods of studying the regulation of secondary metabolism in medicinal plants.

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Squalestatin-induced production of taxol and baccatin in cell suspension culture of yew (Taxus baccata L.)

Cited by 16 publications

References 37 publications

miR156 switches on vegetative phase change under the regulation of redox signals in apple seedlings

miR156 switches on vegetative phase change under the regulation of redox signals in apple seedlings

Salicylic acid as an effective elicitor for improved taxol production in endophytic fungus Pestalotiopsis microspora

The regulatory mechanism of fungal elicitor-induced secondary metabolite biosynthesis in medical plants

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