Squamous cell carcinoma: infiltrating monocyte/macrophage subpopulations express functional mature phenotype

Abstract

Summary Biopsies from 26 patients with advanced stage squamous cell carcinoma of the head and neck were investigated to determine the intensity of the inflammatory cellular infiltrate and the expression of leucocyte antigens. Mononuclear Tumour growth is influenced by cells and/or products of the tumour's microenvironment. These influences are mainly due to cells of the defence system (Boheim et al., 1987;Kopper & Lapis, 1985;Zeromski et al., 1986;Kabawat et al., 1983). In vitro assays have demonstrated a significant role for T cells, natural killer (NK) and lymphokine-activated killer (LAK) cells in killing and lysing of neoplastic cells (Patek & Collins, 1988; Strohme et al., 1987;Whiteside et al., 1988;Vinzenz & Micksche, 1987;Gottlinger et al., 1985). Activated macrophages (Me), also, can bind to and destroy neoplastic cells in vitro and in vivo (Fidler & Schroit, 1984;Fidler & Schroit, 1988;Kopper & Lapis, 1985). Furthermore, MO, via cytokines, are capable of enhancing lymphocyte-mediated immunity (Bentzen, 1988). On the other hand, depending on their localisation in tissue, MO may enhance rather than inhibit tumour growth, possible by triggering local immunosuppression (Gronberg et al., 1989;Kopper & Lapis, 1985;Yamanaka et al., 1988;Kronke, 1988). Considering this bimodal role of MS in immunesurveillance of tumours in vitro we focused our studies on the phenotype and surface receptor expression of MO, which could be involved in local immunesurveillance of malignant growths. Patients and methods PatientsThe study group consisted of 28 patients with histologically confirmed diagnosis of a squamous cell carcinoma of the upper gastro-intestinal and respiratory tract with varying degree of differentiation (

Covalent linking of proteins and cytokines to suture: Enhancing the immune response of head and neck cancer patients

“…CD4+ cells are primarily located in the tumor stroma, whereas the CD8+ cells are located within the tumor parenchyma 46 . Natural killer (NK) cells, 65 dendritic cells, 68 and macrophages 47,69,70 comprise approximately 5% of the population. Approximately 40% of the T cells present express activation marks of human leukocyte antigen (HLA)‐DR, IL‐2R, or transferrin 71 …”

MicroRNA-21 expression and susceptibility to HPV-induced carcinogenesis — role of microenvironment in K14-HPV16 mice model

“…Many cancers arise from sites of infection, chronic irritation and inflammation (Coussens & Werb, 2002). Among other phenomena, inflammation promotes stromal remodeling and inflammatory cell infiltration (Neuchrist et al, 1990;Ortegel, Staren, Faber, Warren, & Braun, 2000), including monocytes/macrophages, B and T lymphocytes, NK cells, and neutrophils. Our results confirm that tumour progression is accompanied by increased inflammatory cell infiltration, especially macrophages and neutrophils.…”

Macrophage and dendritic cell infiltration in head and neck squamous-cell carcinoma; an immunohistochemical study

“…There is experimental evidence that the cell-mediated imlnune system plays an important role in the defence against head and neck squamous carcinoma cells [4,8,9,15,22]. Cell-mediated immune functions are executed by T lymphocytes, monocytes, macrophages and dendritic cells.…”

“…Neuchrist et al [15], also using immunohistology, showed that most tumor-infiltrating macrophages are of a functionally mature phenotype. The distribution these authors described is similar to our findings, with an extensive stromal macrophage invasion and some infiltration into the areas of the malignant epithelial cells (with a wide inter-tumoral variation).…”