Squaraine-Based Polymer Dots with Narrow, Bright Near-Infrared Fluorescence for Biological Applications

Wu, I‐Chin; Yu, Jian; Ye, Fangmao; Rong, Yu; Gallina, Maria Elena; Fujimoto, Bryant S.; Zhang, Yong; Chan, Yang-Hsiang; Sun, Wei; Zhou, Xinghua; Wu, Changfeng; Chiu, Daniel T.

doi:10.1021/ja5123045

Cited by 147 publications

(132 citation statements)

References 34 publications

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“…We chose the well-documented reprecipitation approach here because the technique is very simple but versatile; it is widely employed in many biomedical research studies, including many recent works. 43-47 Fig. 1a and Fig.…”

Section: Resultsmentioning

confidence: 99%

Self-carried curcumin nanoparticles for in vitro and in vivo cancer therapy with real-time monitoring of drug release

et al. 2015

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The use of different nanocarriers for delivering hydrophobic pharmaceutical agents to tumor sites has garnered major attention. Despite the merits of these nanocarriers, further studies are needed to improve their drug loading capacities (which are typically <10%) and reduce their potential systemic toxicity. Therefore, the development of alternative self-carried nanodrug delivery strategies without using inert carriers is highly desirable. In this study, we developed a self-carried curcumin (Cur) nanodrug for highly effective cancer therapy in vitro and in vivo with real-time monitoring of drug release. With a biocompatible C18PMH-PEG functionalization, the Cur nanoparticles (NPs) showed excellent dispersibility and outstanding stability in physiological environments with drug loading capacities >78 wt.%. Both confocal microscopy and flow cytometry confirmed the cellular fluorescence “OFF-ON” activation and real-time monitoring of the Cur molecule release. In vitro and in vivo experiments clearly show that the therapeutic efficacy of the PEGylated Cur NPs is considerably better than that of free Cur. This self-carried strategy with real-time monitoring of drug release may open a new way for simultaneous cancer therapy and monitoring.

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Section: Resultsmentioning

confidence: 99%

Self-carried curcumin nanoparticles for in vitro and in vivo cancer therapy with real-time monitoring of drug release

et al. 2015

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“…44,52,56 Single-Particle Fluorescence Brightness Comparison. As a new type of fluorescent probe, single-particle brightness is one of the important properties for Pdots to be evaluated.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Quinoxaline-Based Polymer Dots with Ultrabright Red to Near-Infrared Fluorescence for In Vivo Biological Imaging

et al. 2015

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This article describes the design and synthesis of quinoxaline-based semiconducting polymer dots (Pdots) that exhibit near-infrared fluorescence, ultrahigh brightness, large Stokes shifts, and excellent cellular targeting capability. We also introduced fluorine atoms and long alkyl chains into polymer backbones and systematically investigated their effect on the fluorescence quantum yields of Pdots. These new series of quinoxaline-based Pdots have a fluorescence quantum yield as high as 47% with a Stokes shift larger than 150 nm. Single-particle analysis reveals that the average per-particle brightness of the Pdots is at least 6 times higher than that of the commercially available quantum dots. We further demonstrated the use of this new class of quinoxaline-based Pdots for effective and specific cellular and subcellular labeling without any noticeable nonspecific binding. Moreover, the cytotoxicity of Pdots were evaluated on HeLa cells and zebrafish embryos to demonstrate their great biocompatibility. By taking advantage of their extreme brightness and minimal cytotoxicity, we performed, for the first time, in vivo microangiography imaging on living zebrafish embryos using Pdots. These quinoxaline-based NIR-fluorescent Pdots are anticipated to find broad use in a variety of in vitro and in vivo biological research.

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“…In particular, Pdots with different luminescent wavelengths have been reported successfully. To date, the major technique challenges that lie ahead of us for the development of Pdots is to design and synthesis a high fluorescence probe in red wavelength range since red wavelength region is more useful for the analysis of biological systems [21].…”

Section: Introductionmentioning

confidence: 99%

Bright red-emitting polymer dots for specific cellular imaging

et al. 2015

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A new conjugated polymer, poly[{2-methoxy-5-(2-ethylhexyloxy)-1,4-(1-cyanovinylphenylene)}-alt-co-{2,5-bis(N,N 0 -diphenylamino)-1,4-phenylene}] (DB-CN-PPV), with strong UV absorption and high fluorescence in red wavelength region, is self-assembled into nanoparticles (polymer dots, Pdots) in aqueous solution upon rapid mixing of the organic solution with water. Different-sized Pdots were obtained by varying the organic solvent percent in the mixture solution and further characterized by dynamic light scattering and transmission electron microscopy. Spectroscopic measurements indicate that the DB-CN-PPV Pdots exhibit a bright red-emitting and a quantum yield of *27 %. Single-particle imaging and analyses indicate the brightness of DB-CN-PPV Pdots under 405 nm excitation is four times brighter than that of the well-known inorganic quantum dot probes, Qdot655, under same excitation light. The successful bioconjugation and cellular imaging suggest DB-CN-PPV Pdots have potential application for biological fluorescence imaging.

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Squaraine-Based Polymer Dots with Narrow, Bright Near-Infrared Fluorescence for Biological Applications

Cited by 147 publications

References 34 publications

Self-carried curcumin nanoparticles for in vitro and in vivo cancer therapy with real-time monitoring of drug release

Self-carried curcumin nanoparticles for in vitro and in vivo cancer therapy with real-time monitoring of drug release

Quinoxaline-Based Polymer Dots with Ultrabright Red to Near-Infrared Fluorescence for In Vivo Biological Imaging

Bright red-emitting polymer dots for specific cellular imaging

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