Square acrylamide gel columns and a novel gel slicer

Aronson, John N.; Borris, David P.

doi:10.1016/0003-2697(67)90051-6

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Disc-electrophoresis1

Polyacrylamide1

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1968

1976

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Cited by 21 publications

(2 citation statements)

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“…The proteins were stained with Comassie Brilliant Blue. Gel slicing was performed by a razor blade set (19).…”

Section: Disc-electrophoresismentioning

confidence: 99%

7-Methylguanine specific tRNA-methyltransferase from Escherichia coli

Aschhoff

Elten

Arnold

et al. 1976

Nucleic Acids Research

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A 7-methylguanine (m7G) specific tRNA methyltransferase from E. coli MRE 600 was purified about 1000 fold by affinity chromatography on Sepharose bound with normal E. coli tRNA. The purified enzyme catalyzes exclusively the formation of m7G in submethylated bulk tRNA of E. coli K12 met- rel-. The purified enzyme transfers the methyl group from S-adenosyl-methionine to initiator tRNA of B. subtilis and 0.8 moles m7G residues are formed per mole tRNA. It is suggested that the enzyme specifically recognizes the extra arm unpaired guanylate residue.

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“…The proteins were stained with Comassie Brilliant Blue. Gel slicing was performed by a razor blade set (19).…”

Section: Disc-electrophoresismentioning

confidence: 99%

7-Methylguanine specific tRNA-methyltransferase from Escherichia coli

Aschhoff

Elten

Arnold

et al. 1976

Nucleic Acids Research

View full text Add to dashboard Cite

show abstract

“…Attempts have been made to measure radioisotope labeling in the many fine bands of an electrophoresis gel. The methods consist of mechanical extrusion (2) or slicing the gel crosswise into small segments (3)(4)(5)(6)(7)(8) and subjecting them to one of the following treatments: solubilization (913) or combustion (14) of the gel, elution of protein by diffusion (15)(16)(17)(18) or electrophoresis (4,19), or replacement of water in the gel by scintillation solvent (20). Most of these methods are tedious and suffer some loss of resolution.…”

Section: Polyacrylamidementioning

confidence: 99%