Square-wave cathodic adsorptive stripping voltammetric determination of 3-hydroxyflavone, Morin and Hesperidin in bulk form and biological fluids in absence and presence of Cu(II)

Abstract

Devido à atividade farmacológica dos flavonóides, na presente investigação, foi desenvolvido um método eficiente e econômico para a determinação de traços dos mesmos. Neste contexto, o comportamento interfacial de três flavonóides, 3-hidroxiflavona (3HF), morin e hesperidina (Hesp) foi estudado em eletrodo de gota pendente de mercúrio (HMDE) por voltametria ac (fase sensível) e voltametria cíclica (CV). Os flavonóides investigados mostraram-se fortemente absorvidos, o que é um pré-requisito para a aplicação de voltametria adsortiva de redissolução catódica, na determinação de alguns flavonóides. Baseado no caráter de adsorção dos flavonóides investigados, na superfície de HMDE, foi descrito um procedimento de voltametria adsortiva de redissolução catódica com onda quadrada (SWCASV), validado, simples, rápido e sensível, para a quantificação dos flavonóides sob investigação na forma granel e em fluidos biológicos. Além disso, a acumulação adsortiva controlada do complexo de Cu(II) de flavonóides foi também obtida e usada para a determinação indireta de 3HF, morin e Hesp via redução do complexo formado. As condições operacionais e de solução para a determinação quantitativa ultra-traço dos compostos flavonóides investigados, foram otimizadas na ausência e presença de Cu(II). Os limites de detecção de 4,4 × 10 -9 , 7,19 × 10 -9 e 7,54 × 10 -9 mol L -1 para 3HF, morin e Hesp, respectivamente, na forma granel, foram atingidos usando SWCASV.As flavonoids show pharmacological activity, in the present investigation, an economical and efficient method for their trace determination was developed. In this context, the interfacial behavior of three flavonoids, 3-hydroxyflavone (3HF), morin and hesperidin (Hesp) was studied on the hanging mercury dropping electrode (HMDE) by phase sensitive ac voltammetry and cyclic voltammetry (CV). The investigated flavonoids were strongly adsorbed which is the prerequisite step for applying the cathodic adsorption stripping voltammetric determination of some flavonoids. Based on the adsorption character of investigated flavonoids onto the surface of the HMDE, validated, simple, fast and sensitive square-wave cathodic adsorptive stripping voltammetric (SWCASV) procedure for the quantification of the flavonoids under investigation in bulk form and in biological fluids was described. Moreover, controlled adsorptive accumulation of the Cu(II) complex of flavonoids was also achieved to assay indirect determination of 3HF, morin and Hesp via the reduction of the formed complex. Operational and solution conditions for the quantitative ultra-trace determination of the investigated flavonoids were optimized in the absence and presence of Cu(II). Limits of detection of 4.4 × 10 -9 , 7.19 × 10 -9 and 7.54 × 10 -9 mol L -1 3HF, morin and Hesp in bulk form were achieved using SWCASV, respectively.

Synthesis of Cr(III)-Morin Complex: Characterization and Antioxidant Study

“…The biological and medicinal properties of flavonoids have been reviewed extensively, with wealth of data on their activity as reducing agents, hydrogen-donating antioxidants, and singlet oxygen quenchers [ 1 – 5 ]. They also show beneficial effects in age-associated diseases such as cardiovascular and cerebrovascular diseases, some forms of cancer, and Parkinson's and Alzheimer's diseases [ 6 ]. Many flavonoids are natural chelators and flavonoid metal complexes have showed significantly higher cytotoxic activity than those of the parent flavonoids.…”

Rapid Voltammetric Screening Method for the Assessment of Bioflavonoid Content Using the Disposable Bare Pencil Graphite Electrode

“…By selecting the proper working conditions (main oxidation signal, DPV, H type PGE, BRB pH 1.81), HESP could be quantified over two orders of magnitude with a LoD of 8.58 × 10 −8 mol/L HESP, which could be lowered to 1.90 × 10 −8 mol/L HESP by applying adsorptive stripping voltammetry on the reduction signal. From the only 14 reports related to HESP, voltammetric determination found in the literature and presented in Table 3, only four of them used un-modified/untreated working electrodes, namely HMDE [34,41,42] and BDDE [5], none of them being a carbon-based electrode. Despite the fact that modified working electrodes were employed, half of the 14 reported methods have higher LODs as that obtained without accumulation (DPV) in our study.…”

β-Cyclodextrin-PANI decorated pencil graphite electrode for the electrochemical sensing of morin in almonds and mulberry leaves

“…Literature study shows that not many electrochemical methods are available for the analysis of morin. Electrochemically prepared electrodes for determination of morin include vanadium pentoxide nanoflake film decorated glassy carbon electrode (GCE) [11], polyvinylpyrolidone (PVP) doped carbon paste electrode, Pt electrode [12], bare glassy carbon electrode (GCE) [13] and hanging mercury drop electrode [14], Ir decorated poly(3,4-ethylenedioxythiophene) on carbon fiber paper (Ir-PEDOT/ CFP) [15], 2,5-dimercapto-1,3,4-thiadazole modified carbon fiber paper (p-DMTD/CFP) [16] are also reported. An electrochemical sensing method with molecular imprinting mechanism using Pd nanoparticles-porous graphene carbon nanotubes (CNTs)/GCE has also been listed for quercetin [17].…”