Square-Wave Voltammetry of Human Blood Serum

Abstract

A study on voltammetric analysis of whole blood serum is presented using advanced square-wave voltammetry at an edge plane pyrolytic graphite electrode. The results demonstrate that even in a complex medium like human blood serum, electrochemical characterization can be achieved through the use of advanced voltammetric techniques in conjunction with an appropriate commercially available electrode, such as the edge plane pyrolytic graphite electrode, which boasts superior electrocatalytic properties. Without undergoing any chemical treatment of the serum sample, the square-wave voltammetry technique reveals, for the first time, the electrode reactions of uric acid, bilirubin, and albumin in a single experiment, as represented by well-defined, separated, and intense voltammetric signals. All electrode processes are surface-confined, indicating that the edge plane sites of the electrode serve as an ideal platform for the competitive adsorption of electroactive species, despite the extensive chemical complexity of the serum samples. The speed and differential nature of square-wave voltammetry are crucial for obtaining an outstanding resolution of the voltammetric peaks, maintaining the quasi-reversible nature of the underlying electrode processes, while reducing the impact of follow-up chemical reactions that are coupled to the initial electron transfer for all three detected species, and minimizing fouling of the electrode surface.

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“…However, the low selectivity of voltammetry biosensors in complex media (i.e., blood serum) poses a significant challenge. This is particularly relevant due to the presence of multiple redox-active species and the sensing mechanism employed by these biosensor structures [79]. Besides, the size and amount of nanostructures have significant effects on the sensitivity of biosensors, which can affect the sensor simplicity and reproducibility.…”

“…Besides, the size and amount of nanostructures have significant effects on the sensitivity of biosensors, which can affect the sensor simplicity and reproducibility. Recently, a study has shown that using edge-plane pyrolytic graphite electrodes relying on an advanced SWV method can address some issues for electrochemically characterizing human serum without pre-treatment of the samples or surface modification [79]. In this spectroscopy-like method, the role of choosing electrodes and parameter changes in SW pulses has been studied under different serum samples.…”