Src64 is involved in fusome development and karyosome formation during Drosophila oogenesis

Djagaeva, Inna; Doronkin, Sergey; Beckendorf, Steven K.

doi:10.1016/j.ydbio.2005.05.012

Cited by 31 publications

(37 citation statements)

References 60 publications

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“…It remains unclear how this apparently germ-line-specific gene affects both nuclear morphology and Grk function. A similar phenotype has been observed in mutants defective in actin dynamics such as Src64, Tec29, and Kelch Djagaeva et al 2005).…”

Section: Resultssupporting

confidence: 71%

“…Like mutants in src64B and tec29, which show a similar phenotype, Trin may mediate chromatin remodeling in the oocyte by regulating the actin cytoskeleton (Simon et al 1983;Guarnieri et al 1998;Roulier et al 1998;Djagaeva et al 2005). In this context, the DV phenotype of eggs from trin mutants may be an indirect effect due to defects in actin cytoskeleton function (Djagaeva et al 2005;Miralles and Visa 2006). The production of collapsed eggs by trin mutant germ-line clones is consistent with this idea (Table 2).…”

Section: Discussionsupporting

confidence: 55%

“…In contrast to nds, trin mutants do not delay meiotic restriction and show defects in the karyosome in spite of normal Grk levels. Like mutants in src64B and tec29, which show a similar phenotype, Trin may mediate chromatin remodeling in the oocyte by regulating the actin cytoskeleton (Simon et al 1983;Guarnieri et al 1998;Roulier et al 1998;Djagaeva et al 2005). In this context, the DV phenotype of eggs from trin mutants may be an indirect effect due to defects in actin cytoskeleton function (Djagaeva et al 2005;Miralles and Visa 2006).…”

Section: Discussionmentioning

confidence: 99%

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A Maternal Screen for Genes Regulating Drosophila Oocyte Polarity Uncovers New Steps in Meiotic Progression

2007

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Meiotic checkpoints monitor chromosome status to ensure correct homologous recombination, genomic integrity, and chromosome segregation. In Drosophila, the persistent presence of double-strand DNA breaks (DSB) activates the ATR/Mei-41 checkpoint, delays progression through meiosis, and causes defects in DNA condensation of the oocyte nucleus, the karyosome. Checkpoint activation has also been linked to decreased levels of the TGFa-like molecule Gurken, which controls normal eggshell patterning. We used this easy-toscore eggshell phenotype in a germ-line mosaic screen in Drosophila to identify new genes affecting meiotic progression, DNA condensation, and Gurken signaling. One hundred eighteen new ventralizing mutants on the second chromosome fell into 17 complementation groups. Here we describe the analysis of 8 complementation groups, including Kinesin heavy chain, the SR protein kinase cuaba, the cohesin-related gene dPds5/cohiba, and the Tudor-domain gene montecristo. Our findings challenge the hypothesis that checkpoint activation upon persistent DSBs is exclusively mediated by ATR/Mei-41 kinase and instead reveal a more complex network of interactions that link DSB formation, checkpoint activation, meiotic delay, DNA condensation, and Gurken protein synthesis.

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Section: Resultssupporting

confidence: 71%

Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 99%

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A Maternal Screen for Genes Regulating Drosophila Oocyte Polarity Uncovers New Steps in Meiotic Progression

2007

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“…4, Table 3) (Djagaeva et al, 2005). By contrast to Src64 LOF , wild-type egg chambers always contained 16 germ cells (Table 3).…”

Section: Src64 Mutant Egg Chambers Have Aberrant Cell Numbersmentioning

confidence: 95%

Cskdifferentially regulatesSrc64during distinct morphological events inDrosophilagerm cells

et al. 2006

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The Src family protein tyrosine kinases (SFKs) are crucial regulators of cellular morphology. In Drosophila, Src64 controls complex morphological events that occur during oogenesis. Recent studies have identified key Src64-dependent mechanisms that regulate actin cytoskeletal dynamics during the growth of actin-rich ring canals, which act as intercellular bridges between germ cells. By contrast, the molecular mechanisms that regulate Src64 activity levels and potential roles for Src64 in additional morphological events in the ovary have not been defined. In this report, we demonstrate that regulation of Src64 by Drosophila C-terminal-Src Kinase (Csk) contributes to the packaging of germline cysts by overlying somatic follicle cells during egg chamber formation. These results uncover novel roles for both Csk and Src64 in a dynamic event that involves adhesion, communication between cell types and control of cell motility. Strikingly, Src64 and Csk function in the germline to control packaging, not in migrating follicle cells, suggesting novel functions for this signaling cassette in regulating dynamic adhesion. In contrast to the role played by Csk in the regulation of Src64 activity during packaging, Csk is dispensable for ring canal growth control, indicating that distinct mechanisms control Src64 activity during different morphological events.

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“…src64 mRNA is present in early precellularization embryos, before zygotic transcription begins, indicating that src64 mRNA is maternally deposited in the embryo. src64 D17 , a mutation that deletes the first two exons of src64, has been used in many studies on src64 (Djagaeva et al, 2005;Dodson et al, 1998;Guarnieri et al, 1998;Kelso et al, 2002;Lu et al, 2004;Roulier et al, 1998;Thomas and Wieschaus, 2004). We suspected that src64 D17 mutants retain some src64 activity because a trace of Src64 protein was detected on Western blots , and the src64 KO knockout mutation causes more severe ring canal growth defects and egg-laying defects than the src64 D17 mutation (O'Reilly et al, 2006).…”

mentioning

confidence: 99%

Maternal and zygotic requirements for src64 during Drosophila cellularization

Strong¹,

Thomas²

2011

Genesis

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Summary: Cellularization of the multinucleate Drosophila embryo occurs shortly after zygotic transcription begins. During cellular blastoderm morphogenesis, the microfilament cytoskeleton undergoes extensive reorganization. This cytoskeletal reorganization includes synchronized microfilament contraction regulated by src64, a gene encoding a Src nonreceptor tyrosine kinase. We report that src64 is maternally expressed in the Drosophila embryo and acts primarily as a maternal gene during cellularization. However, we show that src64 has some zygotic activity during late cellularization. By using compound chromosomes to generate embryos with wild-type levels of maternal src64 activity, we show that this zygotic activity is normally nonessential. We also report the identification of an alternate src64 transcript. Expression of this transcript is not affected by the src64 D17 deletion mutation, explaining the presence of low levels of src64 activity observed in src64 D17 mutants. genesis 49:912-918, 2011. V V C 2011 Wiley Periodicals, Inc.

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Src64 is involved in fusome development and karyosome formation during Drosophila oogenesis

Cited by 31 publications

References 60 publications

A Maternal Screen for Genes Regulating Drosophila Oocyte Polarity Uncovers New Steps in Meiotic Progression

A Maternal Screen for Genes Regulating Drosophila Oocyte Polarity Uncovers New Steps in Meiotic Progression

Cskdifferentially regulatesSrc64during distinct morphological events inDrosophilagerm cells

Maternal and zygotic requirements for src64 during Drosophila cellularization

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