sRNA21, a novel small RNA, protects Mycobacterium abscessus against oxidative stress

Abstract

Background During infection, Mycobacterium abscessus encounters numerous environmental changes and adapts to them using a variety of complex mechanisms. Non‐coding small RNAs (sRNAs) have been shown in other bacteria to be involved in post‐transcriptional regulatory pathways, including environmental stress adaptation. However, the potential role of sRNAs in the resistance to oxidative stress in M. abscessus was not clearly described. Methods In the present study, we analyzed putative sRNAs identified by RNA‐sequencing (RNA‐seq) experiments in M. abscessus ATCC_19977 under oxidative stress, and the transcription profiles of sRNAs with differential expression were verified by quantitative reverse transcription‐PCR (qRT‐PCR). Six sRNA overexpression strains were constructed, and the differences in growth curves between these strains and the control strain were verified. An upregulated sRNA under oxidative stress was selected and named sRNA21. The survival ability of the sRNA21 overexpression strain was assessed, and computer‐based approaches were used to predict the targets and pathways regulated by sRNA21. The total ATP production and NAD+/NADH ratio of the sRNA21 overexpression strain were measured. The expression level of antioxidase‐related genes and the activity of antioxidase were tested to confirm the interaction of sRNA21 with the predicted target genes in silico. Results In total, 14 putative sRNAs were identified under oxidative stress, and the qRT‐PCR analysis of six sRNAs showed comparable results to RNA‐seq assays. Overexpression of sRNA21 in M. abscessus increased cell growth rate and intracellular ATP level before and after peroxide exposure. The expression of genes encoding alkyl hydroperoxidase and superoxide dismutase was significantly increased, and superoxide dismutase activity was enhanced in the sRNA21 overexpression strain. Meanwhile, after sRNA21 overexpression, the intracellular NAD+/NADH ratio decreased, indicating changes in redox homeostasis. Conclusions Our findings show that sRNA21 is an oxidative stress‐induced sRNA that increases M. abscessus survival and promotes the expression of antioxidant enzymes under oxidative stress. These findings may provide new insights into the adaptive transcriptional response of M. abscessus to oxidative stress.