SRXRF microprobe as a technique for studying elements distribution in Elsholtzia splendens

Shi, Jiyan; Chen, Y.X; Huang, Yongshun; He, Wei

doi:10.1016/j.micron.2004.02.011

Cited by 43 publications

(26 citation statements)

References 46 publications

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“…In the mature tissue behind the growth zone, metal concentrations are in the range for root metal concentration reported by other investigators (Wu et al 1975;Shi et al 2004;Chen et al 2004;O'Dell et al 2007). Average Cu and Zn concentrations per gram dry weight in the region 2.8-3.2 mm (rapidly growing tissue) and 8.8-9.2 mm (growth finished; root hairs forming) have relationships similar to those found on the fresh weight basis (compare Fig.…”

Section: Spatial Distribution Of Cu and Zn In Root Growth Zonessupporting

confidence: 82%

Laser ablation ICP-MS reveals patterns of copper differing from zinc in growth zones of cucumber roots

Shi

Gras²,

Silk

2009

Planta

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Laser ablation coupled with inductively coupled plasma-mass spectrometry was used to find Cu and Zn concentration in surface tissue along a longitudinal developmental gradient with meristem, rapidly elongating tissue, and nongrowing tissue in a model system of seedling roots of Cucumis sativus L. (cucumber). Tissue metal accumulation was determined for roots of seedlings growing on cellulosic germination paper treated with nutrient solution (controls), and also treated with concentrations of Zn (40 ppm) and Cu (10 ppm) that reduced growth. Cu content of all roots is highest at the apex and falls sharply to lower values by 2 mm from the root tip. In contrast, at moderate Zn availability (0.07 ppm), Zn content rises from the apex to 2 mm then falls throughout the remainder of the growth zone. At high external Zn the spatial pattern resembles that of Cu. Cucumber root growth zones accumulate more of each metal with higher external availability. Metal deposition rates were calculated using a continuity equation with data on local metal content and growth velocity. Deposition rates of both metals are generally highest in the rapidly elongating region, 1.5-3.5 mm, even where metal concentration is decreasing with position and root age and even when the accumulation is inhibitory to growth.

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Section: Spatial Distribution Of Cu and Zn In Root Growth Zonessupporting

confidence: 82%

Laser ablation ICP-MS reveals patterns of copper differing from zinc in growth zones of cucumber roots

Shi

Gras²,

Silk

2009

Planta

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“…It is an indicator plant of Cu mines and widely distributed in Cu-mining wastes and Cu-contaminated soils in China. Recently, populations of E. haichowensis from Cu mine deposits have been characterized for metal accumulation (Lou et al 2004;Shi et al 2004). Copper tolerance in E. haichowensis appears to be based mainly on an exclusion mechanism; the roots can accumulate high levels of Cu, but its translocation to the shoots is limited (Lou et al 2004;Song et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Excess copper induces accumulation of hydrogen peroxide and increases lipid peroxidation and total activity of copper–zinc superoxide dismutase in roots of Elsholtzia haichowensis

Zhang

Xia

Wang

et al. 2007

Planta

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“…Knowledge of the subcellular localization and identification of heavy metals can provide essential information on metal toxicity and bioaccumulation mechanisms . The Cu and other elements localization and heavy mental tolerance mechanisms in plants have been studied by electron dispersive X-ray spectroscopy (EDXS; Kupper et al 2000;Monni et al 2002), electron energy loss spectroscopy (EELS; Neumann et al 1995;Barcelo and Poschenrieder 1999;Rau et al 2006), proton induced X-ray analysis (PIXE; Krä mer et al 1997) and Synchrotron radiation X-ray fluorescence microprobe technique (Shi et al 2004). EDXS and EELS of these techniques were most usually used to reveal subcellular localization of Cu and other metals (MacFarlane and Burchett 2000;Brunner and Frey 2000;Monni et al 2002;Rau et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Accumulation, subcellular localization and ecophysiological responses to copper stress in two Daucus carota L. populations

Zhou

Xie

et al. 2007

Plant Soil

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Copper accumulation, subcellular localization and ecophysiological responses to excess copper were investigated using pot culture experiments with two Daucus carota L. populations, from a copper mine and an uncontaminated field site, respectively. Significant differences of malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ) concentrations and antioxidant enzyme [superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX)] activities of leaves under Cu treatment were observed between the two populations. At high Cu concentrations (400 and 800 mg kg -1 ), a significant increase in contents of MDA and H 2 O 2 but a significant decrease in activities of SOD, CAT and APX were observed in uncontaminated population. Contrarily, the population from copper mine maintained a lower level of MDA and H 2 O 2 but higher activities of SOD, CAT and APX. Copper accumulation in roots and shoots increased significantly with the increase of copper concentrations in soils in the two populations. No significant difference of the total Cu in roots and shoots was found between the two populations at same copper treatment. There were also no striking differences of cell wall-bound Cu and protoplasts Cu of leaves between the two populations. The difference was that Cu concentration in vacuoles of leaves was 1.5-fold higher in contaminated site (CS) population than in uncontaminated site population. Hence, more efficient vacuolar sequestration for Cu and maintaining high activities of SOD, CAT and APX in the CS population played an important role in maintaining high Cu tolerance.

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SRXRF microprobe as a technique for studying elements distribution in Elsholtzia splendens

Cited by 43 publications

References 46 publications

Laser ablation ICP-MS reveals patterns of copper differing from zinc in growth zones of cucumber roots

Laser ablation ICP-MS reveals patterns of copper differing from zinc in growth zones of cucumber roots

Excess copper induces accumulation of hydrogen peroxide and increases lipid peroxidation and total activity of copper–zinc superoxide dismutase in roots of Elsholtzia haichowensis

Accumulation, subcellular localization and ecophysiological responses to copper stress in two Daucus carota L. populations

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