SRY-related (<i>Sox</i>) genes in the genome of European Atlantic sturgeon (<i>Acipenser sturio</i>)

Hett, Anne Kathrin; Ludwig, Arne

doi:10.1139/g04-112

Cited by 42 publications

(28 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, sturgeon geneticists have failed to find sex-determining genes despite using a plethora of molecular approaches (Hett and Ludwig 2005;Wuertz et al 2006;Keyvanshokooh et al 2007;McCormick et al 2008). Similarly, the research described herein provides no evidence for a master gene whose presence or absence determines lake sturgeon sex.…”

Section: Sex-determining Genesmentioning

confidence: 73%

Discovery and evaluation of candidate sex-determining genes and xenobiotics in the gonads of lake sturgeon (Acipenser fulvescens)

2010

View full text Add to dashboard Cite

Modern pyrosequencing has the potential to uncover many interesting aspects of genome evolution, even in lineages where genomic resources are scarce. In particular, 454 pyrosequencing of nonmodel species has been used to characterize expressed sequence tags, xenobiotics, gene ontologies, and relative levels of gene expression. Herein, we use pyrosequencing to study the evolution of genes expressed in the gonads of a polyploid fish, the lake sturgeon (Acipenser fulvescens). Using 454 pyrosequencing of transcribed genes, we produced more than 125 MB of sequence data from 473,577 high-quality sequencing reads. Sequences that passed stringent quality control thresholds were assembled into 12,791 male contigs and 32,629 female contigs. Average depth of coverage was 4.2 x for the male assembly and 5.5x for the female assembly. Analytical rarefaction indicates that our assemblies include most of the genes expressed in lake sturgeon gonads. Over 86,700 sequencing reads were assigned gene ontologies, many to general housekeeping genes like protein, RNA, and ion binding genes. We searched specifically for sex determining genes and documented significant sex differences in the expression of two genes involved in animal sex determination, DMRT1 and TRA-1. DMRT1 is the master sex determining gene in birds and in medaka (Oryzias latipes) whereas TRA-1 helps direct sexual differentiation in nematodes. We also searched the lake sturgeon assembly for evidence of xenobiotic organisms that may exist as endosymbionts. Our results suggest that exogenous parasites (trematodes) and pathogens (protozoans) apparently have infected lake sturgeon gonads, and the trematodes have horizontally transferred some genes to the lake sturgeon genome.

show abstract

Section: Sex-determining Genesmentioning

confidence: 73%

Discovery and evaluation of candidate sex-determining genes and xenobiotics in the gonads of lake sturgeon (Acipenser fulvescens)

2010

View full text Add to dashboard Cite

show abstract

“…In the family Acipenseridae, species with different ploidy levels are present in most geographic areas of the distribution, suggesting that hybridization events frequently occur in the wild and may lead to change in the ploidy levels. It also indicates that genome duplication is unrestrained by a precise chromosomal sex determination (Hett and Ludwig 2005;Wuertz et al 2006;Keyvanshokooh et al 2007). Studies of sturgeon karyotype are therefore of great relevance to understanding sturgeon genome evolution: the high chromosome number and ploidy complexity bestow a high plasticity to their genome and make them outstanding models to study basic evolutionary problems.…”

Section: Discussionmentioning

confidence: 99%

Evidence of hexaploid karyotype in shortnose sturgeon

Fontana

Congiu

Mudrak

et al. 2008

Genome

View full text Add to dashboard Cite

A karyotype analysis by several staining techniques was carried out on triplicate samples of the shortnose sturgeon, Acipenser brevirostrum. The chromosome number was found to be 2n = 372 +/- 6. A representative karyotype of 374 chromosomes was composed of 178 metacentrics/submetacentrics and 196 telocentrics/acrocentrics and microchromosomes. The signals of fluorescent in situ hybridization (FISH) with a HindIII satellite DNA probe were visible on 14 chromosomes. The signals obtained with a PstI satellite DNA probe appeared on 12 chromosomes. The FISH with a 5S rDNA probe revealed fluorescent signals on 6 chromosomes. These last results, compared with 2 signals in species with about 120 chromosomes and 4 in species with 240, support the hypothesis that A. brevirostrum is a hexaploid species, probably of hybrid origin. Based on these results, we propose a model explaining speciation events occurring in sturgeons by hybridization, genome duplication, and diploidization.

show abstract

“…Several transcription factors in the Sox family [related to sexdetermining region Y (SRY) proteins] are involved in skeletal development in addition to their roles in developmental processes in other tissues (Schilham et al, 1996;Hong and Saint-Jeannet, 2005). Sox4 contains the Sox family characteristic high mobility group (HMG) box, and is highly conserved in human, mouse, chicken (Maschhoff et al, 2003) and fish (Hett and Ludwig, 2005;Mavropoulos et al, 2005), and expressed in brain, gonads, lung, heart and thymus (Schilham et al, 1996). It is implicated in lymphocyte differentiation (Schilham et al, 1997; van de Wetering et al,…”

Section: Introductionmentioning

confidence: 99%

Osteopenia, decreased bone formation and impaired osteoblast development inSox4heterozygous mice

Nissen‐Meyer

Jemtland

Gautvik

et al. 2007

Journal of Cell Science

View full text Add to dashboard Cite

The transcription factor Sox4 is vital for fetal development, as Sox4–/– homozygotes die in utero. Sox4 mRNA is expressed in the early embryonic growth plate and is regulated by parathyroid hormone, but its function in bone modeling/remodeling is unknown. We report that Sox4+/– mice exhibit significantly lower bone mass (by dual-energy X-ray absorptiometry) from an early age, and fail to obtain the peak bone mass of wild-type (WT) animals. Microcomputed tomography (μCT), histomorphometry and biomechanical testing of Sox4+/– bones show reduced trabecular and cortical thickness, growth plate width, ultimate force and stiffness compared with WT. Bone formation rate (BFR) in 3-month-old Sox4+/– mice is 64% lower than in WT. Primary calvarial osteoblasts from Sox4+/– mice demonstrate markedly inhibited proliferation, differentiation and mineralization. In these cultures, osterix (Osx) and osteocalcin (OCN) mRNA expression was reduced, whereas Runx2 mRNA was unaffected. No functional defects were found in osteoclasts. Silencing of Sox4 by siRNA in WT osteoblasts replicated the defects observed in Sox4+/– cells. We demonstrate inhibited formation and altered microarchitecture of bone in Sox4+/– mice versus WT, without apparent defects in bone resorption. Our results implicate the transcription factor Sox4 in regulation of bone formation, by acting upstream of Osx and independent of Runx2.

show abstract

SRY-related (Sox) genes in the genome of European Atlantic sturgeon (Acipenser sturio)

Cited by 42 publications

References 36 publications

Discovery and evaluation of candidate sex-determining genes and xenobiotics in the gonads of lake sturgeon (Acipenser fulvescens)

Discovery and evaluation of candidate sex-determining genes and xenobiotics in the gonads of lake sturgeon (Acipenser fulvescens)

Evidence of hexaploid karyotype in shortnose sturgeon

Osteopenia, decreased bone formation and impaired osteoblast development inSox4heterozygous mice

Contact Info

Product

Resources

About