SSIIIa-RNAi suppression associated changes in rice grain quality and starch biosynthesis metabolism in response to high temperature

Zhao, Qian; Ye, Yu; Han, Zhanyu; Zhou, Lujian; Guan, Xianyue; Pan, Gang; Asad, Muhammad‐Asad‐Ullah; Cheng, Fangmin

doi:10.1016/j.plantsci.2020.110443

Cited by 18 publications

(8 citation statements)

References 32 publications

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“…It is believed that the decrease of SSIIIa may play a key role in those changes of genes. We suggest that the higher expression level of SSI under DS may be caused by the depression of SSIIIa , as it has been demonstrated that the deficiency of SSIIIa can enhance the expression of SSI 23,24 . The downregulation of DBEs ( ISAII and PUL ) is also suggested to be related to the loss of SSIIIa , 24 although the mechanism is still unclear.…”

Section: Resultsmentioning

confidence: 81%

A new insight into the biosynthesis, structure, and functionality of waxy maize starch under drought stress

Zhong

Liu

et al. 2023

J Sci Food Agric

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BACKGROUND Drought stress (DS) is the main abiotic stress that maize suffers during its whole growth period, and maize is also sensitive to DS. It had been demonstrated that DS could improve the quality of normal maize starch. However, waxy maize, which has special properties, has not been explored in depth, which limits the breeding and cultivation of waxy maize varieties and the application of waxy maize starch. Therefore, in this study, we investigated the effects of DS on the biosynthesis, structure, and functionality of waxy maize starch. RESULTS The results showed that DS decreased the expression level of SSIIb, SSIIIa, GBSSIIa, SBEI, SBEIIb, ISAII, and PUL, but increased the expression level of SSI and SBEIIa. DS did not change the average chain length of amylopectin, while increased the relative content of fa chains (RCfa) and decreased the RCfb1 and RCfb3. Furthermore, DS decreased the amylose content, amorphous lamellar distance da, semi‐crystalline repeat distance, and average particle size, whereas it increased the relative crystallinity, crystalline distance dc, the content of rapidly digested starch in the uncooked system and resistant starch content in both the uncooked and cooked system. Conclusions For waxy maize, DS could raise the relative expression level of SSI and SBEIIa, thus increasing RCfa. The larger number of RCfa could create steric hindrance, which can lead to producing more resistant starch in waxy maize starch. © 2023 Society of Chemical Industry.

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Section: Resultsmentioning

confidence: 81%

A new insight into the biosynthesis, structure, and functionality of waxy maize starch under drought stress

Zhong

Liu

et al. 2023

J Sci Food Agric

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“…Furthermore, the current farming system also affects crop production and quality due to over-fertilization of synthetic N, which adversely affects soil quality, the environment, and crop production ( Gu et al, 2015 ). In-addition, it is also well known that the activity of SMEs is affected by the air temperature and the amount of N fertilizer ( Wu et al, 2020 , Zhao et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Carbohydrates (starch) are known to be the ultimate product of photosynthesis ( Everard and Loescher, 2017 ). Previous studies showed that the use of CFs within a definite range can considerably enhance the photosynthetic ability of the leaf and promote the non-structured carbohydrate accumulation (NSC), but the excessive fertilization of CFs reduces the amount of NSC ( Zhu et al, 2017 , Zhao et al, 2020 ). The higher accumulation and translocation of NSC are beneficial to grain filling and it could enhance the physiological strength of grain ( Zheng et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Partial substitution of organic nitrogen with synthetic nitrogen enhances rice yield, grain starch metabolism and related genes expression under the dual cropping system

Iqbal

Xie

et al. 2021

Saudi Journal of Biological Sciences

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Improving grain filling in the presernt farming systems is crucial where grain filling is a concern due to the extreme use of chemical fertilizers (CF). A field experiment was conducted at the experimental station of Guangxi University, China in 2019 to test the hypothesis that cattle manure (CM) and poultry manure (PM) combined with CF could improve rice grain filling rate, yield, biochemical and qualitative attributes. A total of six treatments, i.e., no fertilizer (T 1 ), 100% CF (T 2 ), 60% CM + 40% CF (T 3 ), 30% CM + 70% CF (T 4 ), 60% PM + 40% CF (T 5 ), and 30% PM + 70% CF (T 6 ) were used in this study. Results showed that the combined treatment T 6 increased starch metabolizing enzymes activity (SMEs), such as ADP-glucose phosphorylase (ADGPase) by 8 and 12%, soluble starch synthase (SSS) by 7 and 10%, granule bound starch synthesis (GBSS) by 7 and 9%, and starch branching enzyme (SBE) by 14 and 21% in the early and late seasons, respectively, compared with T 2 . Similarly, higher rice grain yield, grain filling rate, starch, and amylose content were also recorded in combined treatments. In terms of seasons, higher activity of SMEs , grain starch, and amylose content was noted in the late-season compared to the early season. The increment in these traits was mainly attributed to a lower temperature in the late season during the grain filling period. Furthermore, our results suggested that an increment in starch accumulation and grain filling rate were mainly associated with the enhanced sink capacity by regulating key enzyme activities involved in Suc-to-starch conversion. In-addition, RT-qPCR analysis showed higher expression levels of AGPS2b, SSS1, GBSS1, and GBSE11b genes, which resultantly increased the activities of SMEs during the grain filling period under combined treatments. Linear regression analysis revealed that the activity of ADGPase, SSS, GBSS, and SBE were highly positively correlated with starch and amylose accumulation. Thus, we concluded that a combination of 30% N from PM or CM with 70% N from CF is a promising option in terms of improving rice grain yield and quality. Our study provides a sustainable fertilizer management strategy to enhance rice grain yield and quality at the lowest environmental cost.

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“…SS3a and SBE2 are critical genes for AP synthesis. Significant downregulation of SBE2 and SS3a indicated that AP synthesis might be impaired by HT (Zhao et al, 2020). However, AM synthesis should be much more impaired than AP synthesis, since AC was reduced greatly under HT (Lin et al, 2020).…”

Section: Other Starch Biosynthesis Enzymes Affect Rice Ac At Htmentioning

confidence: 99%

Genetic Control and High Temperature Effects on Starch Biosynthesis and Grain Quality in Rice

Jiang

Zhang

et al. 2021

Front. Plant Sci.

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Grain quality is one of the key targets to be improved for rice breeders and covers cooking, eating, nutritional, appearance, milling, and sensory properties. Cooking and eating quality are mostly of concern to consumers and mainly determined by starch structure and composition. Although many starch synthesis enzymes have been identified and starch synthesis system has been established for a long time, novel functions of some starch synthesis genes have continually been found, and many important regulatory factors for seed development and grain quality control have recently been identified. Here, we summarize the progress in this field as comprehensively as possible and hopefully reveal some underlying molecular mechanisms controlling eating quality in rice. The regulatory network of amylose content (AC) determination is emphasized, as AC is the most important index for rice eating quality (REQ). Moreover, the regulatory mechanism of REQ, especially AC influenced by high temperature which is concerned as a most harmful environmental factor during grain filling is highlighted in this review.

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SSIIIa-RNAi suppression associated changes in rice grain quality and starch biosynthesis metabolism in response to high temperature

Cited by 18 publications

References 32 publications

A new insight into the biosynthesis, structure, and functionality of waxy maize starch under drought stress

A new insight into the biosynthesis, structure, and functionality of waxy maize starch under drought stress

Partial substitution of organic nitrogen with synthetic nitrogen enhances rice yield, grain starch metabolism and related genes expression under the dual cropping system

Genetic Control and High Temperature Effects on Starch Biosynthesis and Grain Quality in Rice

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