SSR fingerprinting Chinese peach cultivars and landraces (Prunus persica) and analysis of their genetic relationships

ABSTRACT. Ten SSR loci, previously developed for Prunus, were analyzed to examine genetic relationships among 23 rootstock candidates for sweet and sour cherries, of the species P. avium, P. cerasus, P. mahaleb, and P. angustifolia. Five genotypes of P. laurocerasus, not used as rootstock, were included in the molecular analysis. The number of alleles per locus ranged from 8 to 12, with a mean of 9, while the number of microsatellite genotypes varied from 8 to 17, indicating that the SSRs were highly informative. The degree of heterozygosity (0.61) was high. Clustering analysis resulted in two main clusters. The first cluster was divided into two subclusters; the first subcluster consisted of P. avium and P. cerasus, and the second subcluster consisted of P. laurocerasus. The second cluster was divided into two subclusters. The first subcluster consisted of P. mahaleb genotypes and the second consisted of P. angustifolia genotypes. The reference rootstocks also clustered with their associated botanical species. Unweighted pair-group method with arithmetic mean analysis demonstrated that P. laurocerasus 2157 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 9 (4): 2156-2165 (2010) SSR analysis in Prunus rootstocks genotypes had less genetic variation and that P. avium genotypes were more closely related to P. cerasus. The SSR-based phylogeny was generally consistent with Prunus taxonomy information, suggesting the applicability of SSR analysis for genotyping and phylogenetic studies in the genus Prunus.

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simple sequence repeat-based assessment of genetic relationships among Prunus rootstocks

Turkoglu

Bilgener²,

Erċışlı³

et al. 2010

“…Molecular markers are more advantageous as they are not affected by the environment, can provide a powerful tool for proper characterization of cultivars, are more informative, and do not need extensive observations of mature plants. Various DNA-based markers have been recently developed and used for studies in genetic diversity, fingerprinting and cultivar origin (Fang et al, 2005(Fang et al, , 2006Cheng and Huang, 2009;D'Onofrio et al, 2009;Elidemir and Uzun, 2009;Melgarejo et al, 2009;Papp et al, 2010). Among the several markers available, random amplified polymorphic DNA (RAPD) (William et al, 1990) is useful for cultivar analysis with major advantages in simplicity, efficiency, ease of operation, and non-requirement of any previous sequence information.…”

Section: Introductionmentioning

confidence: 99%

A novel strategy for identification of 47 pomegranate (Punica granatum) cultivars using RAPD markers

Zhang

Tan²,

Cao³

et al. 2012

ABSTRACT. DNA marker can be used for precise plant cultivar identification. However, DNA markers have often not been used effectively for the identification of plant cultivars due to a lack of an effective analysis strategy. We used a novel strategy for effective identification of plant individuals based on a new way of recording DNA fingerprints of the genotyped plants; a cultivar identification diagram can be manually generated and used as key reference information for quick identification of plant and/ or seed samples. Forty-seven pomegranate varieties popularly cultivated in various provinces of China were subjected to RAPD marker analysis. Using the cultivar identification diagram strategy, they were clearly separated by the fingerprints of 11 RAPD primers. The utility and accuracy of the cultivar identification diagram analysis results were confirmed by the identification of three randomly chosen groups of cultivars among the 47 varieties. A novel strategy for identification of P. granatum cultivars 3033

“…Molecular markers have the advantages of not being affected by the environment and being able to provide a powerful tool for proper characterization of cultivars. In recent years, various DNA-based markers have been developed and used for genetic diversity, fingerprinting studies and determination of the origins of cultivars (D'Onofrio et al, 2009;Melgarejo et al, 2009;Cheng and Huang, 2009;Elidemir and Uzun, 2009;Papp et al, 2010), of which random amplified polymorphic DNA (RAPD) (Williams et al, 1990) markers are useful for cultivar analysis with specific advantages in simplicity, efficiency, and non-requirement of any previ-ous sequence information. If some optimization of the RAPD technique, by choosing 11-nt primers and strict screening of PCR annealing temperature for each primer, is done before it is employed in fingerprinting plants, RAPD can be a preferred technique in plant cultivar identification.…”

Section: Introductionmentioning

confidence: 99%

Methodology A new strategy employed for identification of sweet orange cultivars with RAPD markers

Sun

Mu²,

Jiang³

et al. 2012

ABSTRACT. We optimized RAPD techniques by increasing the length of RAPD primers and performing a strict screening of PCR annealing temperature to distinguish 60 sweet orange cultivars from the Research Institute of Pomology at the Chinese Academy of Agricultural Sciences. A new approach called cultivar identification diagram (CID) was used to improve the efficiency of RAPD markers for cultivar identification. Thirteen effective primers were first screened from 54 RAPD arbitrary 11-mer primers based on their amplification products and amplified polymorphic bands; they were then used for PCR amplification of all 60 cultivars. All cultivars were manually and completely separated by the polymorphic bands appearing in DNA fingerprints from 13 primers; a CID of the 60 sweet orange cultivars was then constructed. This CID separated all the cultivars from each other, based on the polymorphic bands; the corresponding primers were marked in the correct positions on the sweet orange CID. The CID strategy facilitates the identification of fruit cultivars with DNA markers. This CID of sweet orange cultivars will be very useful for the protection of cultivar rights and for early identification of seedlings in the nursery industry.