SSR Marker-Based Molecular Characterization of Some Upland Taro (Colocasia esculenta L. Schott) Cultivars of North-East India

menla, Khate; Alam, Shahid; Barooah, Madhumita; Phookan, Deepa Borbora; Kalita, Prakash; Talukdar, Madhumita Choudhury; Luikham, Star

doi:10.20546/ijcmas.2019.806.274

Cited by 5 publications

(2 citation statements)

References 5 publications

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“…A total of 38 scorable alleles were generated with a range of 3-15 alleles per marker and an average of 9.50. This results is in agreement with Khatemenla et al (2019), who recorded 61 scorable amplicons using ten SSR markers subjected to 22 cultivars of taro and average of 6.1. The genetic variation analysis was performed by estimating the allele frequency, gene diversity, observed heterozygosity, xation index and the polymorphic information content (PIC) for each locus.…”

Section: Discussionsupporting

confidence: 91%

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Molecular Characterization of Taro (Colocasia Esculenta (L.) Schott) Using Simple Sequence Repeat (SSR) markers

Amoako,

Koomson,

Ashiagbor

et al. 2024

Preprint

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Taro, Colocasia esculenta (L.) Schott is one of the most valued crops for economic, medicinal, and nutritional roots and tubers globally. However, the crop has not received pronounced research attention to enhance its production and utilization potential, which has resulted in insufficient planting materials for utilization by breeders in Ghana. Genetic diversity characterization of taro at molecular level is important and crucial step for their utilization in breeding and conservation. Hence, this study was carried out to assess the genetic diversity and relationships of 26 accessions of Taro, C. esculenta (L.) Schott collected from Kwame Nkrumah University of Science and Technology, Ghana. A total of 38 putative alleles were amplified at 4 SSR loci with an average of 9.50 alleles per locus. PIC value ranged from 0.44 with primer XUQTEM 97 to 0.89 with primer XUQTEM 73 with an average value of 0.73. Expected Heterozygosity (He) ranged from 0.59 to 0.92. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) clustering using Jaccard's similarity coefficient grouped 26 accessions into eight clusters. The information generated from this investigation will be useful for researchers and breeders for their conservation, Molecular Breeding-based Research & Development and their sustainable future utilization.

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Section: Discussionsupporting

confidence: 91%

“…Avval (2017) also stated that the bigger the PIC, the greater the variability among alleles within the study. Khatemenla et al (2019) also found the SSR markers to be adequately discriminatory in the diversity studies of taro with a PIC of 0.41-0.82 and a mean of 0.68.…”

Section: Discussionmentioning

confidence: 85%

Molecular Characterization of Taro (Colocasia Esculenta (L.) Schott) Using Simple Sequence Repeat (SSR) markers

Amoako,

Koomson,

Ashiagbor

et al. 2024

Preprint

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Assessing the genetic diversity of taro germplasm collection in Vietnam using simple sequence repeat markers

Nguyen,

Le,

et al. 2024

Journal of Crop Improvement

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Identification of Genetic Diversity among Mutant Taro (Colocasia esculenta L. cv WANGI) Using Agro-Morphological Trait and Simple Sequence Repeats (SSR) Molecular Markers

et al. 2022

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Taro (Colocasia esculenta) is one of the traditional crops with enormous sources of dietary fiber, carbohydrates, vitamins, and minerals contents. Mutation breeding using gamma radiation is one of the most preferred approaches used to induce mutation in taro studies. Molecular markers are widely used to detect such induced mutation and genetic diversity in plants. Therefore, the present study was carried out to evaluate genetic diversity among irradiated taro genotypes in comparison with standard taro variety by using simple sequence repeats (SSR). A total of 200 of M1V4 taro genotypes were used in this study derived from segregating population of chronic-gamma irradiated taro cv Wangi with different ranges of gamma dose. The agro-morphological results revealed that genotype exposure in T6 (120.12 Gy) has the highest plant height (54.53 cm), leaf length (32.24 cm), and leaf width (24.87 cm). Corm's weight was decreased significantly with an increased dose of treatment. All mutants recorded a lower number of corm weight as compared with the control genotype. Out of 10 SSR primers tested, 9 primers have successfully amplified 43 amplicons. The polymorphism information content (PIC) values of SSR markers ranged from 0.20 to 0.80. Cluster analysis classified taro into 3 subgroups mutant and parent genotypes. The results clearly showed that SSR markers are important tools to distinguish mutant genotypes and confirmed their usefulness for phylogenetic studies. Finally, the present investigation indicated that genotypes exposed by T6 (120.12 Gy) are promising high-yielding genotypes that can be recommended as new cultivars and possessed an attractive phenotype appropriate for ornamental use.

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SSR Marker-Based Molecular Characterization of Some Upland Taro (Colocasia esculenta L. Schott) Cultivars of North-East India

Cited by 5 publications

References 5 publications

Molecular Characterization of Taro (Colocasia Esculenta (L.) Schott) Using Simple Sequence Repeat (SSR) markers

Molecular Characterization of Taro (Colocasia Esculenta (L.) Schott) Using Simple Sequence Repeat (SSR) markers

Assessing the genetic diversity of taro germplasm collection in Vietnam using simple sequence repeat markers

Identification of Genetic Diversity among Mutant Taro (Colocasia esculenta L. cv WANGI) Using Agro-Morphological Trait and Simple Sequence Repeats (SSR) Molecular Markers

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