Stabilities of hemoglobins A and S in dried blood spots stored under controlled conditions

Adam, Barbara W.; Chafin, D.L.; Jesús, Víctor R. De

doi:10.1016/j.clinbiochem.2013.05.043

Cited by 12 publications

(13 citation statements)

References 10 publications

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“…16 Similar recommendations are also described for storing DBS used in newborn screening and other clinical tests. 32 , 111 , 113 , 114 …”

Section: Storage Conditionsmentioning

confidence: 99%

“…Temperature and humidity conditions directly affect the ability to detect specific amino acids and metabolites routinely measured for newborn screening. 115 , 116 Gene transcriptomics analysis of newborn DBS was more consistent if samples were stored at temperatures less than −20°C immediately after specimen acquisition, 111 with time and temperature imparting various degrees of degradation for specific mRNA profiling targets and housekeeping genes. 112 Lower temperatures is not the solution for all analytes; three polyunsaturated fatty acids used to screen newborns for neural development and visual function were found to have significant degradation after 10 days of storage at −28°C, with a high degree of intraindividual variability, when measured from umbilical blood dried on Ahlstrom 226 cards.…”

Section: Storage Conditionsmentioning

confidence: 99%

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Dried Blood Spots for Global Health Diagnostics and Surveillance: Opportunities and Challenges

Lim

2018

The American Journal of Tropical Medicine and Hygiene

169

143

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Abstract.There is increasing interest in using dried blood spot (DBS) cards to extend the reach of global health and disease surveillance programs to hard-to-reach populations. Conceptually, DBS offers a cost-effective solution for multiple use cases by simplifying logistics for collecting, preserving, and transporting blood specimens in settings with minimal infrastructure. This review describes methods to determine both the reliability of DBS-based bioanalysis for a defined use case and the optimal conditions that minimize pre-analytical sources of data variability. Examples by the newborn screening, drug development, and global health communities are provided in this review of published literature. Sources of variability are linked in most cases, emphasizing the importance of field-to-laboratory standard operating procedures that are evidence based and consider both stability and efficiency of recovery for a specified analyte in defining the type of DBS card, accessories, handling procedures, and storage conditions. Also included in this review are reports where DBS was determined to not be feasible because of technology limitations or physiological properties of a targeted analyte.

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“…16 Similar recommendations are also described for storing DBS used in newborn screening and other clinical tests. 32 , 111 , 113 , 114 …”

Section: Storage Conditionsmentioning

confidence: 99%

Section: Storage Conditionsmentioning

confidence: 99%

Dried Blood Spots for Global Health Diagnostics and Surveillance: Opportunities and Challenges

Lim

2018

The American Journal of Tropical Medicine and Hygiene

169

143

View full text Add to dashboard Cite

show abstract

“…Based on data from the NBSTRN, 18 states currently store DBS specimens at −20 o C, seven states maintain DBS specimens between 4-8 o C, and 16 states use room-temperature storage conditions. Other recent studies examining the stability of markers of metabolic and genetic disorders have demonstrated that DBS storage conditions with lower temperature and humidity are essential for maintaining sample integrity (Adam, Chafin, & De Jesus, 2013;Adam et al, 2011).…”

Section: Storage Of Dbs Cards At −20mentioning

confidence: 99%

Retrospective assessment of prenatal alcohol exposure by detection of phosphatidylethanol in stored dried blood spot cards: An objective method for determining prevalence rates of alcohol consumption during pregnancy

Baldwin

Jones

et al. 2015

IJADR

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Aims: To analyze the efficacy of screening banked newborn dried blood spots (DBS) for detection of phosphatidylethanol (PEth), a direct alcohol biomarker, with the purpose of performing a retrospective assessment of statewide prevalence rates of alcohol consumption in late pregnancy that results in risky prenatal alcohol exposure.Design: Residual DBS samples collected for newborn screening and stored by a state department of public health were examined for concentrations of PEth. The prevalence of prenatal alcohol exposure, as determined by this direct alcohol biomarker, was compared to prevalence rates of alcohol consumption during pregnancy that have been derived from multiple state-based and national studies using maternal self-report surveys.Setting: DBS cards representative of the general newborn population were collected from multiple hospitals across a single midwestern state. Participants:Two hundred fifty anonymous newborn DBS collected for routine metabolic screening in a midwestern state were requested through the Virtual Repository of Dried Blood Spots. Measures:Concentrations of PEth, a highly specific biomarker of alcohol consumption, were analyzed using a liquid chromatography-tandem mass spectrometry method validated by our laboratory. Findings:Of 250 DBS examined, 4% were positive for PEth (PEth ≥ 8 ng/ml) which is indicative of exposure to maternal alcohol consumption during the last month of pregnancy.Conclusions: Detection of PEth from newborn DBS cards can identify prenatal alcohol exposure and also be used for retrospective surveillance of alcohol consumption during the last three to four weeks of pregnancy, using specimens that are collected for routine metabolic screening and stored by many state health departments.

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“…The acquired data were used in previously described statistical procedures [10] for estimating initial marker level in small DBS production batches.…”

Section: Methodsmentioning

confidence: 99%

Galactose-1-phosphate uridyltransferase dried blood spot quality control materials for newborn screening tests

Adam

Flores

Hou

et al. 2015

Clinical Biochemistry

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Objectives We aimed to prepare dried-blood-spot (DBS) quality control (QC) materials for galactose-1-phosphate uridyltransferase (GALT), to evaluate their stability during storage and use, and to evaluate their performance in five DBS GALT test methods. Design and Methods We prepared and characterized GALT-normal and GALT-deficient DBS materials and compared GALT activities in DBSs after predetermined storage intervals at controlled temperatures and humidities. External evaluators documented the suitability of the DBS QC materials for use in five GALT test methods. Results GALT activity losses from DBSs stored in low (<30%) humidity for 14 days at 45°C, 35 days at 37°C, 91 days at room temperature, 182 days at 4°C, and 367 days at −20°C were 54%, 53%, 52% 23%, and 7% respectively. In paired DBSs stored in high humidity (>50%) for identical intervals, losses were: 45°C—68%; 37°C—79%; room temperature—72%, and 4°C—63%. GALT activities in DBSs stored at 4°C were stable throughout 19 excursions to room temperature. Twenty-five of 26 external evaluators, using five different GALT test methods, classified the GALT-deficient DBSs as “outside normal limits”. All evaluators classified the GALT-normal DBSs as “within normal limits”. Conclusions Most of the GALT activity loss from DBSs stored at elevated or room temperature was attributable to the effects of storage temperature. Most of the loss from DBSs stored at 4°C was attributable to the effects of elevated humidity. Loss from DBSs stored at −20°C was insignificant. The DBS materials were suitable for monitoring performance of all five GALT test methods.

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Stabilities of hemoglobins A and S in dried blood spots stored under controlled conditions

Cited by 12 publications

References 10 publications

Dried Blood Spots for Global Health Diagnostics and Surveillance: Opportunities and Challenges

Dried Blood Spots for Global Health Diagnostics and Surveillance: Opportunities and Challenges

Retrospective assessment of prenatal alcohol exposure by detection of phosphatidylethanol in stored dried blood spot cards: An objective method for determining prevalence rates of alcohol consumption during pregnancy

Galactose-1-phosphate uridyltransferase dried blood spot quality control materials for newborn screening tests

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