This paper is concerned with the stability of second-order linear time-varying systems. By utilizing the Lyapunov approach, a generally uniformly asymptotic stability criterion is obtained by adding the system matrices into the quadratic Lyapunov candidate function. In the case of the derivative of the Lyapunov candidate function is semi-positive definite, the stability criterion is also efficient. Based on the proposed results, the systems with uncertain disturbances such as structured independent and structured dependent perturbations are considered. Using the matrix measure and the singular value theory, the bounds of the uncertainties are obtained that guarantee the system uniformly asymptotically stable, while the bounds of state feedback control input are also derived to stabilize the second-order linear time-varying systems. Finally, several numerical examples are given to prove the validity and correctness of the proposed criteria with existing ones.