Study design: A retrospective cohort. Objectives: To report the incidence rates of shoulder injuries diagnosed with magnetic resonance imaging (MRI) in tetraplegic athletes and sedentary tetraplegic individuals. To evaluate whether sport practice increases the risk of shoulder injuries in tetraplegic individuals. Setting: Campinas, Sao Paulo, Brazil. Methods: Ten tetraplegic athletes with traumatic spinal cord injury were selected among quad rugby athletes and had both the shoulders evaluated by MRI. They were compared with 10 sedentary tetraplegic individuals who were submitted to the same radiological protocol. Results: All athletes were male with a mean age of 32.1 years (range 25-44 years, s.d. = 6.44). Time since injury ranged from 6 to 17 years, with a mean value of 9.7 years and s.d. of 3.1 years. All sedentary individuals were male with a mean age of 35.9 years (range 22-47 years, s.d. = 8.36). Statistical analysis showed a protective effect of sport in the development of shoulder injuries, with a weak correlation for infraspinatus and subscapularis tendinopathy (P = 0.09 and P = 0.08, respectively) and muscle atrophy (P = 0.08). There was a strong correlation for acromioclavicular joint (ACJ) and labrum injuries (P = 0.04), with sedentary individuals at a higher risk for these injuries. Conclusion: Tetraplegic athletes and sedentary individuals have a high incidence of supraspinatus tendinosis, bursitis and ACJ degeneration. Statistical analysis showed that there is a possible protective effect of sport in the development of shoulder injuries. Weak evidence was encountered for infraspinatus and subscapularis tendinopathy and muscle atrophy (P = 0.09, P = 0.08 and P = 0.08, respectively). Strong evidence with P = 0.04 suggests that sedentary tetraplegic individuals are at a greater risk for ACJ and labrum injuries.