Stability and instability processes in the calli of Fagopyrum tataricum that have different morphogenic potentials

Betekhtin, Alexander; Piński, Artur; Milewska‐Hendel, Anna; Kurczyńska, Ewa; Hasterok, Robert

doi:10.1007/s11240-019-01575-w

Cited by 9 publications

(9 citation statements)

References 75 publications

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“…Such a detailed analysis of the spatio-temporal chemical composition of the ECM will contribute to the knowledge about the surface layer that covers a callus that has a different morphogenic potential (Šamaj et al 2008;Endress et al 2009;Betekhtin et al 2019). These results are presented for the first time (at least to the best of our knowledge) for the A. arguta callus.…”

Section: Chemical Composition Of Ecm and Cell Wall As A Marker For DImentioning

confidence: 85%

Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta

Popielarska-Konieczna

Sala

Abdullah

et al. 2020

Plant Cell Rep

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Key message Differences in the composition and the structural organisation of the extracellular matrix correlate with the morphogenic competence of the callus tissue that originated from the isolated endosperm of kiwifruit. Abstract The chemical composition and structural organisation of the extracellular matrix, including the cell wall and the layer on its surface, may correspond with the morphogenic competence of a tissue. In the presented study, this relationship was found in the callus tissue that had been differentiated from the isolated endosperm of the kiwiberry, Actinidia arguta. The experimental system was based on callus samples of exactly the same age that had originated from an isolated endosperm but were cultured under controlled conditions promoting either an organogenic or a non-organogenic pathway. The analyses which were performed using bright field, fluorescence and scanning electron microscopy techniques showed significant differences between the two types of calli. The organogenic tissue was compact and the outer walls of the peripheral cells were covered with granular structures. The non-organogenic tissue was composed of loosely attached cells, which were connected via a net-like structure. The extracellular matrices from both the non-and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus. Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes).

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Section: Chemical Composition Of Ecm and Cell Wall As A Marker For DImentioning

confidence: 85%

Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta

Popielarska-Konieczna

Sala

Abdullah

et al. 2020

Plant Cell Rep

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“…As has been shown in previous studies, analyses of the distribution and changes in the signal intensity of epitopes can be compared with the expression profiles of the genes encoding the proteins that are targeted by these antibodies [15,34]. In this work, we focused on the FLA that have been implicated in modulation of signalling upstream of cell wall polymer biosynthesis, remodelling, as well as in the stress response as one of the AGP sub-families [35,36,37].…”

Section: Discussionmentioning

confidence: 99%

Hydroxyproline-Rich Glycoproteins as Markers of Temperature Stress in the Leaves of Brachypodium distachyon

Piński

Betekhtin

Sala

et al. 2019

IJMS

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Plants frequently encounter diverse abiotic stresses, one of which is environmental thermal stress. To cope with these stresses, plants have developed a range of mechanisms, including altering the cell wall architecture, which is facilitated by the arabinogalactan proteins (AGP) and extensins (EXT). In order to characterise the localisation of the epitopes of the AGP and EXT, which are induced by the stress connected with a low (4 °C) or a high (40 °C) temperature, in the leaves of Brachypodium distachyon, we performed immunohistochemical analyses using the antibodies that bind to selected AGP (JIM8, JIM13, JIM16, LM2 and MAC207), pectin/AGP (LM6) as well as EXT (JIM11, JIM12 and JIM20). The analyses of the epitopes of the AGP indicated their presence in the phloem and in the inner bundle sheath (JIM8, JIM13, JIM16 and LM2). The JIM16 epitope was less abundant in the leaves from the low or high temperature compared to the control leaves. The LM2 epitope was more abundant in the leaves that had been subjected to the high temperatures. In the case of JIM13 and MAC207, no changes were observed at the different temperatures. The epitopes of the EXT were primarily observed in the mesophyll and xylem cells of the major vascular bundle (JIM11, JIM12 and JIM20) and no correlation was observed between the presence of the epitopes and the temperature stress. We also analysed changes in the level of transcript accumulation of some of the genes encoding EXT, EXT-like receptor kinases and AGP in the response to the temperature stress. In both cases, although we observed the upregulation of the genes encoding AGP in stressed plants, the changes were more pronounced at the high temperature. Similar changes were observed in the expression profiles of the EXT and EXT-like receptor kinase genes. Our findings may be relevant for genetic engineering of plants with increased resistance to the temperature stress.

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“…An analysis of the embryogenic callus of B. distahyon revealed that AGP epitopes, which were recognized by the JIM16 and LM2 antibodies, were positive markers of embryogenic cells [ 25 ]. For the Fagopyrum tataricum embryogenic callus, it was demonstrated that the AGPs epitope recognized by the LM2 antibody was a positive marker of the embryogenic cells [ 47 ]. Studies during the SE of Arabidopsis reported that the JIM16 AGP epitope appeared to be a marker of embryogenic cells [ 29 ].…”

Section: Cell Wall Composition As a Marker Of Changes In Cell Fate Du...mentioning

confidence: 99%

“…Extensin/HRGP glycoprotein Musa spp. embryogenic cells and nonembrygenc cells [40] Phalaenopsis embryogenic cells [42] Brachypodium distachyon embryogenic calli [25] Actinidia arguta embryogenic and nonembryonic cells [43] JIM12 Extensin/HRGP glycoprotein Dactylis glomerata embryogenic calli [41] Actinidia arguta embryogenic and nonembryonic cells [43] Brachypodium distachyon nonembryonic cells [25] AGP JIM8 Arabinogalactan Arabidopsis thaliana explant cells [29] Daucus carrota nonembryonic cells [26] JIM4 Arabinogalactan glycoprotein, βGlcA-(1,3)-αGalA-(1,2)-Rha Arabidopsis thaliana explant cells [29] LM2 Arabinogalactan protein, carbohydrate epitope containing β-linked GlcA Quercus suber proembryogenic masses, somatic embryos [28] Brachypodium distachyon embryogenic calli [25] Fagopyrum tataricum embryogenic cells [47] Arabidopsis thaliana explant cells [29] Daucus carrota meristematic/pluripotent cells [26]…”

Section: Jim20mentioning

confidence: 99%

Apoplastic and Symplasmic Markers of Somatic Embryogenesis

Kurczyńska

Godel-Jędrychowska

2023

Plants

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Somatic embryogenesis (SE) is a process that scientists have been trying to understand for many years because, on the one hand, it is a manifestation of the totipotency of plant cells, so it enables the study of the mechanisms regulating this process, and, on the other hand, it is an important method of plant propagation. Using SE in basic research and in practice is invaluable. This article describes the latest, but also historical, information on changes in the chemical composition of the cell wall during the transition of cells from the somatic to embryogenic state, and the importance of symplasmic communication during SE. Among wall chemical components, different pectic, AGP, extensin epitopes, and lipid transfer proteins have been discussed as potential apoplastic markers of explant cells during the acquisition of embryogenic competence. The role of symplasmic communication/isolation during SE has also been discussed, paying particular attention to the formation of symplasmic domains within and between cells that carry out different developmental processes. Information about the number and functionality of plasmodesmata (PD) and callose deposition as the main player in symplasmic isolation has also been presented.

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Stability and instability processes in the calli of Fagopyrum tataricum that have different morphogenic potentials

Cited by 9 publications

References 75 publications

Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta

Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta

Hydroxyproline-Rich Glycoproteins as Markers of Temperature Stress in the Leaves of Brachypodium distachyon

Apoplastic and Symplasmic Markers of Somatic Embryogenesis

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