We calculate the energetic structure of defect cores and propose controlled methods to imprint a nontrivially entangled vortex pair that undergoes non-Abelian vortex reconnection in a biaxial nematic spin-2 condensate. For a singular vortex, we find three superfluid cores in addition to depletion of the condensate density. These exhibit order parameter symmetries that are different from the discrete symmetry of the biaxial nematic phase, forming an interface between the defect and the bulk superfluid. We provide a detailed analysis of phase mixing in the resulting vortex cores and find an instability dependent upon the orientation of the order parameter. We further show that the spin-2 condensate is a promising system for observing spontaneous deformation of a point defect into an "Alice ring" that has so far avoided experimental detection.Topological defects and textures are ubiquitous across physical systems that seemingly have little in common [1], from liquid crystals [2] and superfluids [3] to cosmic strings [4]. They arise generically from symmetries of a ground state that is described by an order parameter [5]-a function parametrizing the set of physically distinguishable, energetically degenerate states. In the simple example of a scalar superfluid, the order parameter is the phase of the macroscopic wave function. More generally it may be a vector or tensor that is symmetric under particular transformations. In a uniaxial nematic (UN), the order parameter is cylindrically symmetric around a locally defined axis. It also exhibits a twofold discrete symmetry under reversal of the cylinder axis, which leads to half-quantum vortices (HQVs) in atomic spinor BoseEinstein condensates (BECs) [6][7][8][9] and superfluid liquid 3 He [10], and to π disclinations in liquid crystals [1,2]. In a biaxial nematic (BN), also the cylindrical symmetry is broken into the fully discrete symmetry of a rectangular brick, with dramatic consequences: the BN is the simplest order parameter that supports non-Abelian vortices that do not commute [5,11]. As a result, colliding non-Abelian vortices cannot reconnect without leaving traces of the process, but must instead form a connecting rung vortex. Noncommuting defects appear as cosmic strings in theories of the early universe [4], and have been predicted in BN liquid crystals [11].Despite long-standing experimental efforts, BN phases in liquid crystals have experimentally proved more elusive than originally anticipated [12]. In atomic systems, the topological classification and dynamics of non-Abelian vortices have theoretically been studied in the cyclic phase of spin-2 [13-16] and in spin-3 BECs [17], though it remains uncertain whether any alkali-metal atoms exhibit the corresponding ground states. Consequently, any physical system where non-Abelian defects may be reliably studied is still lacking.Spin-2 BECs exhibit-in addition to the ferromagnetic (FM) and cyclic phases-both UN and BN phases [18][19][20], which, however, are degenerate at the mean-field level. Beyond mean-fi...