Stability and Intra-Individual Variation of Urinary Malondialdehyde and 2-Naphthol

Lee, Kyung Ho; Kang, Daehee

doi:10.3961/jpmph.2008.41.3.195

Cited by 16 publications

(21 citation statements)

References 30 publications

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“…MDA standards (0.1, 0.5, 1, 2, and 4μmol/L) were prepared with 1,1,3,3-tetramethoxypropane. The LOD was 0.05μmol/L, the correlation for the linearity of the standard curve was0.99 and the CV was less than 10% (20). …”

Section: Methodsmentioning

confidence: 99%

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Breast Cancer and Urinary Biomarkers of Polycyclic Aromatic Hydrocarbon and Oxidative Stress in the Shanghai Women's Health Study

Lee

Shu

Gao

et al. 2010

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Polycyclic aromatic hydrocarbon (PAH) exposure and oxidative stress from such and other exposures have been associated with breast cancer in some studies. To further evaluate the role of PAH metabolites and oxidative stress on the development of breast cancer, we conducted a nested case-control study in the Shanghai Women's Health Study.We measured urinary 1-hydroxypyrene and 2-naphthol as PAH metabolites and urinary levels of 8-hydroxy-2′-deoxyguanosine and malondialdehyde as oxidative stress biomarkers in 327 breast cancer cases and 654 controls in the Shanghai Women's Health Study. Information on demographic characteristics, past medical history, lifestyles, history of menstruation, pregnancy history, eating and drinking habit, history of residence, employment history, family history, husband's information, and physical activity were collected by a self-administered questionnaire.The mean age was 52.3 in breast cancer cases (n = 354) and 52.5 in controls (n = 708). Age at menarche (P = 0.04), months of breast-feeding the first baby (P = 0.05), and grade of education (P trend < 0.01) were significantly different between cases and controls. No association was observed for PAH metabolites and the oxidative stress biomarkers of urinary malondialdehyde and 8-hydroxy-2′-deoxyguanosine and risk of breast cancer.This nested case-control study provides no evidence of association between PAH exposure and oxidative stress and risk of breast cancer in Shanghai women. Cancer Epidemiol Biomarkers Prev; 19(3); 877-83. ©2010 AACR.

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Section: Methodsmentioning

confidence: 99%

“…Excitation/emission wavelengths used in the detection of 2-naphthol was 227/355 nm. The LOD was 0.5ng/ml and the CV was less than 15% (20). …”

Section: Methodsmentioning

confidence: 99%

Breast Cancer and Urinary Biomarkers of Polycyclic Aromatic Hydrocarbon and Oxidative Stress in the Shanghai Women's Health Study

Lee

Shu

Gao

et al. 2010

Cancer Epidemiology, Biomarkers &Amp; Prevention

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“…Lee and Kang [20] n = 10 -2.43 ± 0.19 HPLC-FL Lee and Kang [20] n = 44 -1.52 ± 0.20 HPLC-FL Kil et al [21] n = 500 0.01 -11.86 2.15 ± 1.60 HPLC-FL studies performed with HPLC separation of MDA-TBA2 adduct. Furthermore, it is demonstrated that MDA is stabile in urine at room temperature for 24 h and when stored at -20 °C for 6 months.…”

Section: Resultsmentioning

confidence: 99%

“…Kumar et al [22] observed that MDA is unstable when stored at -20 °C for longer than three weeks, with an observed increase of MDA level in plasma stored for 1 month to 1 year. Lee and Kang [20] showed that MDA is stable in urine at room temperature and at -20 °C for 16 hours. In this study, the stability of MDA in urine was assessed at room temperature for 24 h and when stored at -20 °C for 6 months.…”

Section: Stability Of Mda In Urine Samplesmentioning

confidence: 99%

“…In an epidemiological study conducted on 44 Chinese volunteers (22 children and their mothers; average 23.4 years old, range 10 -46), mean MDA level in urine was 1.52 ± 0.20 µM, determined by HPLC with fluorescent (FL) detector. [20] In the same study, when testing the stability of MDA in urine (at -20 °C for 16 h) mean level of MDA in urine of 10 subjects was as high as 2.43 ± 0.19 µM. In another epidemiological study that included 500 urine samples collected in Korea (including adults, primary and middle school students, and infants), MDA in urine was assessed by HPLC with FL detector and mean MDA concentration was 2.15 ± 1.6 µM (range from 0.01 to 11.86 µM).…”

Section: Urine Samplesmentioning

confidence: 99%

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Spectrophotometric Determination of Malondialdehyde in Urine Suitable for Epidemiological Studies

Weitner¹,

Inić²,

Jablan³

et al. 2016

Croat. Chem. Acta

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A reliable method for spectrophotometric determination of urinary malondialdehyde (MDA), according to the thiobarbituric acid (TBA) assay, is described. To account for matrix interference and differences in individual urine composition, standard addition procedure was applied. The method is adequately selective (LoQ = 0.09 μM in the presence of 0.1 M creatinine and 0.5 M urea) and reliable (within-day and between-day variability of less than 5 %). The mean level of urinary MDA was 1.52 ± 0.73 µM that is in good agreement with spectrofluorometric determination (1.20 ± 0.56 μM; p = 0.085) as well as with previous studies that used HPLC. Furthermore, it is demonstrated that MDA is stabile in urine at room temperature for 24 h and when stored at -20 °C for 6 months. The described method enables simple, rapid and cost-effective determination of urinary MDA as a relevant and non-invasive marker of "whole-body" oxidative stress.

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Methodological confounds of measuring urinary oxidative stress in wild animals

Melvin

Dhirani

Mitchell

et al. 2022

Ecology and Evolution

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Biomarkers of oxidative stress (OS) are useful in addressing a wide range of research questions, but thus far, they have had limited application to wild mammal populations due to a reliance on blood or tissue sampling. A shift toward non‐invasive measurement of OS would allow field ecologists and conservationists to apply this method more readily. However, the impact of methodological confounds on urinary OS measurement under field conditions has never been explicitly investigated. We combined a cross‐sectional analysis with a field experiment to assess the impact of four potential methodological confounds on OS measurements: (1) time of sampling, (2) environmental contamination from foliage; (3) delay between sample collection and flash‐freezing in liquid nitrogen; and (4) sample storage of up to 15 months below −80°C. We measured DNA oxidative damage (8‐hydroxy‐2′‐deoxyguanosine, 8‐OHdG), lipid peroxidation (malondialdehyde, MDA), total antioxidant capacity (TAC), and uric acid (UA) in 167 urine samples collected from wild Zanzibar red colobus ( Piliocolobus kirkii ). We found that MDA was higher in samples collected in the morning than in the afternoon but there were no diurnal patterns in any of the other markers. Contamination of samples from foliage and length of time frozen at −80°C for up to 15 months did not affect OS marker concentrations. Freezing delay did not affect OS levels cross‐sectionally, but OS values from individual samples showed only moderate‐to‐good consistency and substantial rank‐order reversals when exposed to different freezing delays. We recommend that diurnal patterns of OS markers and the impact of storage time before and after freezing on OS marker concentrations be considered when designing sampling protocols. However, given the high stability we observed for four OS markers subject to a variety of putative methodological confounds, we suggest that urinary OS markers provide a valuable addition to the toolkit of field ecologists and conservationists within reasonable methodological constraints.

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Stability and Intra-Individual Variation of Urinary Malondialdehyde and 2-Naphthol

Cited by 16 publications

References 30 publications

Breast Cancer and Urinary Biomarkers of Polycyclic Aromatic Hydrocarbon and Oxidative Stress in the Shanghai Women's Health Study

Breast Cancer and Urinary Biomarkers of Polycyclic Aromatic Hydrocarbon and Oxidative Stress in the Shanghai Women's Health Study

Spectrophotometric Determination of Malondialdehyde in Urine Suitable for Epidemiological Studies

Methodological confounds of measuring urinary oxidative stress in wild animals

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