Control strategy on Rayleigh-Benard convection in rotating nanofluids saturated in anisotropic porous layer heated from below is studied in the presence of uniformly internal heat source for rigid-rigid, free-free, and lower-rigid and upper-free boundaries. Feedback control strategy with an array of sensors situated at the top plate and actuators located at the bottom plate of the nanofluids layer are considered in this study. Linear stability analysis based on normal mode technique has been performed, the eigenvalue problem is obtained numerically by implementing the Galerkin method and computed by using Maple software. Model employed for the nanofluids includes the mechanisms of Brownian motion and thermophoresis. The problem of the onset of convective rolls instabilities in a horizontal porous layer with isothermal boundaries at unequal temperatures known as Horton-Roger-Lapwood model based on the Darcy model for the fluids flow is used. The influences of internal heat source’s strength, modified diffusivity ratio, nanoparticles concentration Darcy-Rayleigh number and nanofluids Lewis number are found to advance the onset of convection, meanwhile the mechanical anisotropy parameter, thermal anisotropy parameter, porosity, rotation, and controller effects are to slow down the process of convective instability. No visible observation on the modified particle density increment and rigid-rigid boundaries are the most stable system compared to free-free and rigid-free boundaries.