Stability determination of solid lipid nanoparticles (SLN TM) in aqueous dispersion after addition of electrolyte

Freitas, C.; Rh, Müller

doi:10.1080/026520499289310

Cited by 87 publications

(25 citation statements)

References 3 publications

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“…The higher electrolyte concentration caused reduction in electrostatic repulsion, which resulted in aggregation of SLNs. 30 …”

Section: Preparation and Characterization Of Slnsmentioning

confidence: 99%

Solid lipid nanoparticles for thermoresponsive targeting: evidence from spectrophotometry, electrochemical, and cytotoxicity studies

Rehman¹,

Ihsan²,

Madni³

et al. 2017

IJN

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Thermoresponsive drug delivery systems are designed for the controlled and targeted release of therapeutic payload. These systems exploit hyperthermic temperatures (>39°C), which may be applied by some external means or due to an encountered symptom in inflammatory diseases such as cancer and arthritis. The objective of this paper was to provide some solid evidence in support of the hypothesis that solid lipid nanoparticles (SLNs) can be used for thermoresponsive targeting by undergoing solid–liquid phase transition at their melting point (MP). Thermoresponsive lipid mixtures were prepared by mixing solid and liquid natural fatty acids, and their MP was measured by differential scanning calorimetry (DSC). SLNs (MP 39°C) containing 5-fluorouracil (5-FU) were synthesized by hot melt encapsulation method, and were found to have spherical shape (transmission electron microscopy studies), desirable size (<200 nm), and enhanced physicochemical stability (Fourier transform infrared spectroscopy analysis). We observed a sustained release pattern (22%–34%) at 37°C (5 hours). On the other hand, >90% drug was released at 39°C after 5 hours, suggesting that the SLNs show thermoresponsive drug release, thus confirming our hypothesis. Drug release from SLNs at 39°C was similar to oleic acid and linoleic acid nanoemulsions used in this study, which further confirmed that thermoresponsive drug release is due to solid–liquid phase transition. Next, a differential pulse voltammetry-based electrochemical chemical detection method was developed for quick and real-time analysis of 5-FU release, which also confirmed thermoresponsive drug release behavior of SLNs. Blank SLNs were found to be biocompatible with human gingival fibroblast cells, although 5-FU-loaded SLNs showed some cytotoxicity after 24 hours. 5-FU-loaded SLNs showed thermoresponsive cytotoxicity to breast cancer cells (MDA-MB-231) as cytotoxicity was higher at 39°C (cell viability 72%–78%) compared to 37°C (cell viability >90%) within 1 hour. In conclusion, this study presents SLNs as a safe, simple, and effective platform for thermoresponsive targeting.

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“…The higher electrolyte concentration caused reduction in electrostatic repulsion, which resulted in aggregation of SLNs. 30 …”

Section: Preparation and Characterization Of Slnsmentioning

confidence: 99%

Solid lipid nanoparticles for thermoresponsive targeting: evidence from spectrophotometry, electrochemical, and cytotoxicity studies

Rehman¹,

Ihsan²,

Madni³

et al. 2017

IJN

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“…As a consequence of this lower j value aggregation may occur. This phenomenon is well known for lipid nanoemulsions [14], as well as for SLN when incorporated into polyacrylate hydrogels [15]. However, the tested lipid nanoparticles made from Dynasan w 116 and Tyloxapol w revealed no sensitivity to exposure to sodium ions.…”

Section: Zeta Potential (J)mentioning

confidence: 92%

Evaluation of the physical stability of SLN and NLC before and after incorporation into hydrogel formulations

Souto

Wissing

Barbosa

et al. 2004

European Journal of Pharmaceutics and Biopharmaceutics

201

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Aqueous dispersions of lipid nanoparticles are being investigated as drug delivery systems for different therapeutic purposes. One of their interesting features is the possibility of topical use, for which these systems have to be incorporated into commonly used dermal carriers, such as creams or hydrogels, in order to have a proper semisolid consistency. For the present investigation four different gel-forming agents (xanthan gum, hydroxyethylcellulose 4000, Carbopol w 943 and chitosan) were selected for hydrogel preparation. Aqueous dispersions of lipid nanoparticles-solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC)-made from tripalmitin were prepared by hot high pressure homogenization and then incorporated into the freshly prepared hydrogels. NLC differ from SLN due to the presence of a liquid lipid (Miglyol w 812) in the lipid matrix. Lipid nanoparticles were physically characterized before and after their incorporation into hydrogels. By means of rheological investigations it could be demonstrated that physical properties of the dispersed lipid phase have a great impact on the rheological properties of the prepared semisolid formulations. By employing an oscillation frequency sweep test, significant differences in elastic response of SLN and NLC aqueous dispersions could be observed. q

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“…High temperature, light, and shear stress are some of the promoters of gelation (63). Gel formation may also occur due to high lipid concentration, high ionic strength (64), rapid crystallization of the lipid (57) and intense contact of the SLN dispersion with other surfaces, like the surface of the packing material (65). All gelation promoters increase the kinetic energy of the particles and favor their collision.…”

Section: Lipid Modificationsmentioning

confidence: 99%

Solid lipid based nanocarriers: An overview / Nanonosači na bazi čvrstih lipida: Pregled

Pardeshi¹,

Rajput²,

Belgamwar³

et al. 2012

Acta Pharmaceutica

185

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In the era of nanoparticulate controlled and site specific drug delivery systems, use of solid lipids to produce first generation lipid nanoparticles, solid lipid nanoparticles (SLN), became a revolutionary approach in the early nineties. The present review is designed to provide an insight into how SLN are finding a niche as promising nanovectors and forms a sound basis to troubleshoot the existing problems associated with traditional systems. Herein, authors had tried to highlight the frontline aspects prominent to SLN. An updated list of lipids, advanced forms of SLN, methods of preparation, characterization parameters, and various routes of administration of SLN are explored in-depth. Stability, toxicity, stealthing, targeting efficiency and other prospectives of SLN are also discussed in detail. The present discussion embodies the potential of SLN, now being looked up by various research groups around the world for their utility in the core areas of pharmaceutical sciences, thereby urging pharmaceutical industries to foster their scale-up.

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Stability determination of solid lipid nanoparticles (SLN TM) in aqueous dispersion after addition of electrolyte

Cited by 87 publications

References 3 publications

Solid lipid nanoparticles for thermoresponsive targeting: evidence from spectrophotometry, electrochemical, and cytotoxicity studies

Solid lipid nanoparticles for thermoresponsive targeting: evidence from spectrophotometry, electrochemical, and cytotoxicity studies

Evaluation of the physical stability of SLN and NLC before and after incorporation into hydrogel formulations

Solid lipid based nanocarriers: An overview / Nanonosači na bazi čvrstih lipida: Pregled

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