Stability-indicating densitometric high-performance thin-layer chromatographic method for the quantitative analysis of biomarker naringin in the leaves and stems of<i>Rumex vesicarius</i>L.

Alam, Perwez; Siddiqui, Nasir A.; Al‐Rehaily, Adnan J.; Alajmi, Mohamed F.; Basudan, Omar A.; Khan, Tajdar Husain

doi:10.1556/jpc.27.2014.3.10

Cited by 24 publications

(1 citation statement)

References 13 publications

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“…Modern analytical techniques such as HPLC and HPTLC can be employed for separation and identification followed by both qualitative and quantitative analysis of different constituents in the plant extract or formulations [4][5][6][7]. Over the past decades HPLC has proved its analytical excellence due to its high sensitivity and accuracy and therefore it has been a method of choice for estimation of phytoconstituents [5] and on the other side, HPTLC being a very popular tool for quality-control of herbal drugs, enjoys some important advantages such as high sample-throughput, minimum sample clean-up and less time consumption which makes it highly cost effective and even low volume of mobile phase is sufficient for simultaneous analysis of mixture of different compounds [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

RP-HPLC and HPTLC Identification and Quantification of Flavonoids in the Leaves Extract of Bauhinia acuminata Linn. (Caesalpiniaceae)

Chakraborty¹,

Bala²,

Das

2021

JPRI

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Aims: The present investigation is aimed at the identification and quantification of flavonoids in the methanol extract of leaves of Bauhinia acuminata Linn. (Caesalpiniaceae) by a validated HPTLC method and confirmation of the same by RP-HPLC. Methodology: CAMAG HPTLC system with VisionCATS software and HPLC Agilent Infinity 1260 were employed for the study. Pre-coated silica gel 60 F254 plates were used as stationary phase and Toluene : Ethyl acetate : Formic acid (5:4:0.2, v/v/v) was used as mobile phase in HPTLC while in HPLC Thermo hypersil BDS C18 column and Methanol and 0.4% Phosphoric acid (65 : 35) were used as stationary and mobile phase respectively. Results: Among different standards used in HPTLC, only quercetin was found to be present and further quantitatively analyzed. The method shows good correlation coefficient (R2 ≥ 0.99) and linearity in the range of 0.5 to 5.0 µg/band. The concentration of quercetin in the test extract was found to be 0.514 µg/mg of the extract. The LOD and LOQ values were 0.84 µg/band and 2.59 µg/band respectively. The RP-HPLC study also represented good % RSD of Retention time (0.025) and Area (0.09) which signifies the high precision and repeatability. The assay result (4.99 %) also indicated good presence of the quercetin in the extract. Conclusion: The methods were rapid, cost effective and may be employed for quality-control and standardization of quercetin in different plant extracts.

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