Stability of a salicylate-based poly(anhydride-ester) to electron beam and gamma radiation

Abstract

The effect of electron beam and gamma radiation on the physicochemical properties of a salicylate-based poly(anhydride-ester) was studied by exposing polymers to 0 (control), 25 and 50 kGy. After radiation exposure, salicylic acid release in vitro was monitored to assess any changes in drug release profiles. Molecular weight, glass transition temperature and decomposition temperature were evaluated for polymer chain scission and/or crosslinking as well as changes in thermal properties. Proton nuclear magnetic resonance and infrared spectroscopies were also used to determine polymer degradation and/or chain scission. In vitro cell studies were performed to identify cytocompatibility following radiation exposure. These studies demonstrate that the physicochemical properties of the polymer are not substantially affected by exposure to electron beam and gamma radiation.

A novel application of electrospun silk fibroin/poly(l-lactic acid-co-ε-caprolactone) scaffolds for conjunctiva reconstruction

“…37 H&E staining showed that with the extension of the implantation time in vivo, the bers of the scaffolds gradually degrade, and the degradation products of SF/PLCL scaffolds are amino acids from SF, and lactic acid and caproic acid from PLCL, and these are metabolizable and nontoxic. 38 Thus, these ndings indicate that SF/PLCL scaffolds could promote conjunctiva formation.…”

In vitro degradation of an aromatic polyanhydride with enhanced thermal properties

“…Electron beam and gamma irradiation were chosen as other common sterilizing methods involve heat and/or moisture (e.g., dry heat, autoclaving, and ethylene oxide), which cause polyanhydride degradation. [15] Polymer samples were subjected to 0, 25, or 50 kGy doses (untreated, standard sterilizing dose, and maximum sterilizing dose, respectively) of either electron beam or gamma radiation. The irradiated polymers were then analyzed for changes in molecular weight or degradation rates.…”

Salicylic acid‐based poly(anhydride‐ester) nerve guidance conduits: Impact of localized drug release on nerve regeneration

“…Degradation media (1 mL) was removed and replaced by 1 mL PBS every 24 h for the first 7 days, then once more every 7 days until the experiment was finished. The spent media was analyzed by UV ( λ = 303 nm) with a Lamda XLS UV/vis spectrophotometer (Perkin Elmer, Waltham, MA) to determine the SA release as previously described . A calibration curve was made from SA solutions of known concentration from the average UV data ( n = 3 for each time point).…”