Stability of beta-carotene in spray dried preparation of Rhodotorula glutinis mutant 32

Abstract

Aims: To obtain b-carotene-rich dry cell preparation from mutant 32 of Rhodotorula glutinis and determination of its pigment stability. Methods and Results: The mutant 32 of R. glutinis was grown in a 14 l stirred tank fermenter. Cell mass was concentrated 10-fold by cross-flow microfiltration and then spray dried. Butylated hydroxy toluene (BHT) and D D-tocopherol were used as protecting agents. A two-level, three-variable, factorial optimization was performed to achieve moisture-free, non-viable and b-carotene-rich feed additive. Conclusions: The b-carotene and cell mass in stirred tank fermenter were found to be 54 ± 5 mg l )1 and 12AE8 ± 2 g l )1 , respectively. In the presence of BHT, 97 ± 3% (w/w) b-carotene was recovered for all the inlet temperatures studied. The best b-carotene and yeast powder recoveries were obtained at 160°C, 11AE6% (w/v) cell mass concentration and 1 g l )1 BHT. The pigments inside dried yeast powder were stable in dark and cold condition for at least 10 weeks. The purified b-carotene got almost totally denatured, under similar conditions of storage, within 76 h. Significance and Impact of the Study: Spray dried and stable preparation of b-carotene-rich yeast, R. glutinis can provide alternative source of b-carotene for use in animal nutrition.

Pilot-scale production and liquid formulation of Rhodotorula minuta, a potential biocontrol agent of mango anthracnose

“…We used high-speed centrifugation in order to concentrate the biomass and for preparing either the fresh preparations or the formulations containing glycerol and xanthan. This was basically carried out because of the availability of the equipment in our pilot plant; however, microfiltration can be used as a cheaper alternative for processing large volumes of broth (Bhosale et al 2003). Figure 5 shows that the yeast was effective in controlling anthracnose in two of the three tests conducted in two different harvest sessions.…”

Carotenoids from Rhodotorula and Phaffia: yeasts of biotechnological importance

“…dendrourhous or Rhodotorula species has been hindered absorption of carotenoids, due to the yeast`s thick cell wall. The biotechnology industry has developed diVerent means of pigment liberation by the yeast including optimization of drying conditions, mechanical breakage, microwave treatment and enzyme treatment [149][150][151][152][153][154][155]. When disrupted cells P. rhodozyma, without cell walls, are added to the diets of animals, astaxanthin is readily absorbed from the gut; it eVectively colors the Xesh of penreared salmonids [148], and also helps impart a desirable golden color to the egg yolk and Xesh of poultry [156].…”

Structural Characterization and Bioactivities of Red Pigment from Marine Rhodotorula glutinis

“…The attention has been paid to the yeast because it has been used industrially in the production of carotenoid pigments and as a biocontrol agent (an antibiosis metabolite for post-harvest spoilage diseases of fruits). Table 2 Compounds produced from Rhodotorula glutinis [22][23][24][25][26][27][28][29][30][31][32][33][34][35] …”

“…Lipids [22][23][24] Fatty acid [25,26] Oil [27] Lipase [28] Thymine hidroxylase [29,30] Tannase [31] Pectinase [31] Invertase [32] Carotenoids [25,[33][34][35] The use of R. glutinis biosynthesis of β-carotene owns the following advantages: [36] non-toxic bacteria as well as rich in protein and vitamins; using bagasse, waste molasses for culture; low cost, short cycle; simple fermentation control; potential production; the entire cell as a carotenoid single cell protein; used as feed additives or aquaculture bait and do not have to extract the pigment. Moreover, astaxanthin is the main pigment of R. glutinis, with significant antioxidant properties.…”