Stability of coagulation parameters in plasma samples at room temperature after one freeze/thaw cycle

Abstract

In clinical laboratories, the pre-analytical step is crucial for coagulation assays because it can influence the results and consequently clinical decision-making. Pre-analytical variables, such as blood sampling, transportation, processing and storage (with time and temperature as variables), influence the haemostasis results. 1 Many data are available on the stability of coagulation parameters and storage conditions (e.g. room temperature (RT) versus 4°C, stability over time) for fresh plasma and whole blood. For instance, in fresh plasma stored at RT, prothrombin time (PT), clauss fibrinogen, antithrombin (AT), factor (F) II, FV, FVII, FX and D-dimers are stable up to 24 h, but activated partial thromboplastin time (aPTT) only for 8 h. [2][3][4][5][6] The impact of the freezing and storage temperatures (i.e. −84°C versus

Comparison of analytical performances between clot waveform analysis and FibWave in edoxaban‐treated patients and healthy controls

“…The aim was to reduce the risk of bias due to the stability of edoxaban in plasma after thawing. Recent data showed that the coagulation parameters' stability in samples containing apixaban or rivaroxaban, at room temperature after one freeze/thaw cycle, was 3 and 2 h, respectively 33 . To our knowledge, no data are available for edoxaban in the same context, but we took this precaution as a conservative approach.…”

Effect of tube filling on plasma freezing for coagulation testing

“…However, owing to the centralization of laboratories and the need for specialized analyses, controls, or clinical studies, plasma samples may be frozen and stored for later analysis. The stability of frozen samples has been extensively studied and may vary with the storage temperature (−20 • C or −80 • C) or the thawing mode [4,5].…”