Stability of different influenza subtypes: How can high hydrostatic pressure be a useful tool for vaccine development?

Dumard, Carlos H.; Barroso, Shana Priscila Coutinho; Santos, Ana C. Vicente; Alves, Nathalia S.; Couceiro, J. N. S. S.; Gomes, André M. O.; Santos, Paulo Sérgio Faro; Silva, Jerson L.; Oliveira, Andréa C.

doi:10.1016/j.bpc.2017.04.002

Cited by 6 publications

(3 citation statements)

References 52 publications

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“…Viral inactivation occurs when the proteins on the surface of the particle change shape, and as a result, lose their ability to function properly, removing their ability to infect cells (Keller et al 2018;Snyder et al 2019;Whitehurst et al 2007). Understanding viral inactivation is important for determining transmission of viruses (Yue et al 2019;Pinon and Vialette 2018;Predmore et al 2015), but also plays a role in vaccine development (Dumard et al 2017;Silva et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Energy Requirements for Loss of Viral Infectivity

Rowell

Dobrovolny

2020

Food Environ Virol

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Outside the host, viruses will eventually lose their ability to infect cells due to conformational changes that occur to proteins on the viral capsid. In order to undergo a conformational change, these proteins require energy to activate the chemical reaction that leads to the conformational change. In this study, data from the literature is used to calculate the energy required for viral inactivation for a variety of different viruses by means of the Arrhenius equation. We find that some viruses (rhinovirus, poliovirus, human immunodeficiency virus, Alkhumra hemorrhagic fever virus, and hepatitis A virus) have high inactivation energies, indicative of breaking of a chemical double bond. We also find that several viruses (respiratory syncytial virus, poliovirus, and norovirus) have nonlinear Arrhenius plots, suggesting that there is more than a single pathway for inactivation of these viruses.

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Section: Introductionmentioning

confidence: 99%

Energy Requirements for Loss of Viral Infectivity

Rowell

Dobrovolny

2020

Food Environ Virol

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“…Interestingly, virus re-associate under their fusogenic state under pressure, a less infectious and highly immunogenic form [144][145][146]. This is why high pressure has been suggested for antiviral vaccine development.…”

Section: Antiviral Vaccinesmentioning

confidence: 99%

High-pressure adaptation of extremophiles and biotechnological applications

Salvador-Castell

Oger

Peters

2020

Physiological and Biotechnological Aspects of Extremophiles

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During the last decades, high pressure has been an important physical parameter not only to study biomolecules, but also for its biotechnological applications. High pressure affects organism's ability to survive by altering most of cell's macromolecules. These effects can be used, for example, to inactivate microorganisms, enhance enzymatic reactions or to modulate cell activities. Moreover, some organisms are capable to growth under high pressures thanks to their adaptation at all cellular levels. Such adaptation confers a wide range of potentially interesting macromolecules still to be discovered. In this chapter, we firstly present the different effects of pressure on cells and the diverse strategies used to cope against this harsh environment. Secondly, we explored the pressure biotechnological applications on pressure-sensitive and adaptedpressure organisms.

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“…Promising results have already been obtained with pressurized and inactivated influenza A H3N8 virus in studies that support the idea that non-human viruses can be used to produce vaccines for humans [29–31]. Here, we describe the effects of hydrostatic pressure (from 0 to 2.9 kbar) on the structure, morphology and functional characteristics of avian influenza A H3N8 virus by using fluorescence spectroscopy, light scattering and electron microscopy.…”

Section: Introductionmentioning

confidence: 96%

Inactivation of avian influenza viruses by hydrostatic pressure as a potential vaccine development approach

Barroso

Santos

et al. 2021

Access Microbiology

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Vaccines are a recommended strategy for controlling influenza A infections in humans and animals. Here, we describe the effects of hydrostatic pressure on the structure, morphology and functional characteristics of avian influenza A H3N8 virus. The effect of hydrostatic pressure for 3 h on H3N8 virus revealed that the particles were resistant to this condition, and the virus displayed only a discrete conformational change. We found that pressure of 3 kbar applied for 6 h was able to inhibit haemagglutination and infectivity while virus replication was no longer observed, suggesting that full virus inactivation occurred at this point. However, the neuraminidase activity was not affected at this approach suggesting the maintenance of neutralizing antibody epitopes in this key antigen. Our data bring important information for the area of structural virology of enveloped particles and support the idea of applying pressure-induced inactivation as a tool for vaccine production.

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Stability of different influenza subtypes: How can high hydrostatic pressure be a useful tool for vaccine development?

Cited by 6 publications

References 52 publications

Energy Requirements for Loss of Viral Infectivity

Energy Requirements for Loss of Viral Infectivity

High-pressure adaptation of extremophiles and biotechnological applications

Inactivation of avian influenza viruses by hydrostatic pressure as a potential vaccine development approach

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