Stability of exosomes in the postmortem serum and preliminary study on exosomal miRNA expression profiling in serum from myocardial infarction cadavers

Kanno, Sanae; Sakamoto, Tsubasa; Fukuta, Mamiko; Kato, Hideaki; Aoki, Yasuhiro

doi:10.1007/s00414-022-02913-y

Cited by 9 publications

(2 citation statements)

References 39 publications

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“…One of the most interesting reports about the diagnostic role of miRNAs in SCD cases is Kanno's study, which showed that if serum samples are stored at below 20 • C, the exosomes and their miRNA content remain stably preserved for at least 3 days [61]. In the same study, S. Kanno and colleagues examined the application of exosomal miRNA for autopsy diagnosis.…”

Section: Role Of Circulating Mirna In Scdmentioning

confidence: 99%

The Role of miRNA Expression Profile in Sudden Cardiac Death Cases

Bernini Di Michele,

Onofri,

Pesaresi

et al. 2023

Genes

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Sudden cardiac death (SCD) is one of the leading causes of death in the world and for this reason it has attracted the attention of numerous researchers in the field of legal medicine. It is not easy to determine the cause in a SCD case and the available methods used for diagnosis cannot always give an exhaustive answer. In addition, the molecular analysis of genes does not lead to a clear conclusion, but it could be interesting to focus attention on the expression level of miRNAs, a class of non-coding RNA of about 22 nucleotides. The role of miRNAs is to regulate the gene expression through complementary binding to 3′-untraslated regions of miRNAs, leading to the inhibition of translation or to mRNA degradation. In recent years, several studies were performed with the aim of exploring the use of these molecules as biomarkers for SCD cases, and to also distinguish the causes that lead to cardiac death. In this review, we summarize experiments, evidence, and results of different studies on the implication of miRNAs in SCD cases. We discuss the different biological starting materials with their respective advantages and disadvantages, studying miRNA expression on tissue (fresh-frozen tissue and FFPE tissue), circulating cell-free miRNAs in blood of patients affected by cardiac disease at high risk of SCD, and exosomal miRNAs analyzed from serum of people who died from SCD.

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Section: Role Of Circulating Mirna In Scdmentioning

confidence: 99%

The Role of miRNA Expression Profile in Sudden Cardiac Death Cases

Bernini Di Michele,

Onofri,

Pesaresi

et al. 2023

Genes

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show abstract

“…Exosomes are considered potential biomarkers for the diagnosis and monitoring of myocardial ischemia and infarction [115,116]. This is of value because of the limitations in sensitivity and specificity and delay in detectable change in the standard marker, troponin T [117].…”

Section: Exosomes In Myocardial Infarctionmentioning

confidence: 99%

Exosomes in Cardiovascular Disease: From Mechanism to Therapeutic Target

et al. 2023

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Cardiovascular disease (CVD) is the leading cause of morbidity and mortality globally. In recent decades, clinical research has made significant advances, resulting in improved survival and recovery rates for patients with CVD. Despite this progress, there is substantial residual CVD risk and an unmet need for better treatment. The complex and multifaceted pathophysiological mechanisms underlying the development of CVD pose a challenge for researchers seeking effective therapeutic interventions. Consequently, exosomes have emerged as a new focus for CVD research because their role as intercellular communicators gives them the potential to act as noninvasive diagnostic biomarkers and therapeutic nanocarriers. In the heart and vasculature, cell types such as cardiomyocytes, endothelial cells, vascular smooth muscle, cardiac fibroblasts, inflammatory cells, and resident stem cells are involved in cardiac homeostasis via the release of exosomes. Exosomes encapsulate cell-type specific miRNAs, and this miRNA content fluctuates in response to the pathophysiological setting of the heart, indicating that the pathways affected by these differentially expressed miRNAs may be targets for new treatments. This review discusses a number of miRNAs and the evidence that supports their clinical relevance in CVD. The latest technologies in applying exosomal vesicles as cargo delivery vehicles for gene therapy, tissue regeneration, and cell repair are described.

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The Detection of Urinary Exosomal miRNAs for Cancer Diagnostics and Prognostics

Kim

Kang

et al. 2023

BioChip J

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Stability of exosomes in the postmortem serum and preliminary study on exosomal miRNA expression profiling in serum from myocardial infarction cadavers

Cited by 9 publications

References 39 publications

The Role of miRNA Expression Profile in Sudden Cardiac Death Cases

The Role of miRNA Expression Profile in Sudden Cardiac Death Cases

Exosomes in Cardiovascular Disease: From Mechanism to Therapeutic Target

The Detection of Urinary Exosomal miRNAs for Cancer Diagnostics and Prognostics

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