Stability of hCG free β-subunit and β-core fragment in urine

Cole, Laurence A.

doi:10.1002/(sici)1097-0223(199702)17:2<185::aid-pd50>3.0.co;2-m

Cited by 8 publications

(1 citation statement)

References 10 publications

(13 reference statements)

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“…The core fragment may be an especially useful marker because it is often observable in urine even when its precursor is undetectable in the serum , O'Connor et al 1988, Rinne et al 1999; moreover, it is exceptionally stable upon storage (de Medeiros et al. 1991, Cole 1997, Mulder et al 1997. Our hypothesis that differential binding of GNA mainly reflects differences in glycosylation on the core fragment needs to be examined by using a hCGβcf-specific capture antibody (O'Connor et al 1994).…”

Section: Discussionmentioning

confidence: 97%

Determination of hyperglycosylated human chorionic gonadotropin produced by malignant gestational trophoblastic neoplasias and male germ cell tumors using a lectin-based immunoassay and surface plasmon resonance

Kelly

Birken

Puett

2007

Molecular and Cellular Endocrinology

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The ability to reliably detect aberrant glycosylation of human chorionic gonadotropin (hCG) may have profound implications for the diagnosis and monitoring of malignant gestational trophoblastic neoplasia, germ cell tumors, other malignancies, and pregnancy complications. To become a clinically useful assay, however, this discrimination of glycoforms should be possible on minimally treated biological specimens. Towards this end, we have developed a lectin-based sandwich-type immunoassay to compare the glycosylation patterns of hCG among urine specimens from patients presenting with a normal pregnancy, invasive mole, choriocarcinoma, and male germ cell tumors using carbohydrate-free antibody fragments as capture reagents and a panel of eight lectins, five recognizing neutral sugars and three recognizing sialic acid. There was no significant difference in the binding of any of the lectins to hCG in the urine of women over the gestational range of 6 -38 weeks. Three lectins, however, exhibited differential binding to urinary hCG derived from these normal pregnant controls and that from patients with malignant forms of gestational trophoblastic disease and male germ cell tumors. Galanthus nivalis agglutinin and Maackia amurensis lectin, which bind terminal mannose and α(2-3)sialic acid, respectively, preferentially bound pregnancyderived hCG, whereas the lectin, wheat germ agglutinin, which binds sialic acid and β(1-4)Nacetylglucosamine, exhibited decreased binding to pregnancy-derived hCG compared to that from patients with male germ cell tumors and malignant gestational trophoblastic neoplasia. The differential binding observed with these three promising lectins is most encouraging and warrants further examination. The experimental paradigm also holds promise for the development of comparable assays for other glycosylated tumor markers.

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Section: Discussionmentioning

confidence: 97%

Determination of hyperglycosylated human chorionic gonadotropin produced by malignant gestational trophoblastic neoplasias and male germ cell tumors using a lectin-based immunoassay and surface plasmon resonance

Kelly

Birken

Puett

2007

Molecular and Cellular Endocrinology

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Comparison of 12 assays for detecting hCG and related molecules in urine samples from Down syndrome pregnancies

et al. 1997

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Urine is a new medium for Down syndrome testing. In an effort to determine the best type of human chorionic gonadotropin (hCG)‐related immunoassay for urine testing, we examined 14 Down syndrome and 91 unaffected pregnancy urine samples with 12 established assays. The assays included (a) those that detect hCG β‐core fragment only; (b) those that detect β‐core fragment with less than 18 per cent free β‐subunit cross‐reactivity; (c) that which equally detects free β‐subunit and β‐core fragment; and (d) those that detect hCG, free β‐subunit, or combinations thereof. The seven type a and b assays had the highest sensitivity for Down syndrome. The median MOM for Down syndrome was 5·93 (range 4·73–7·53). At a 10 per cent false‐positive rate, the median observed detection rate was 93 per cent (range 79–100 per cent) and the median predicted detection rate was 85 per cent (range 69–96 per cent). The assays that did not mainly detect β‐core fragment (types c and d) had poorer screening performance. The median MOM for Down syndrome was 2·70 (range 2·16–3·63 MOM). At a 10 per cent false‐positive rate, the median observed detection rate was 50 per cent (range 36–64 per cent) and the median predicted detection rate was 37 per cent (range 21–62 per cent). We infer that the assays that only detect β‐core fragment, or β‐core fragment with minor free β‐subunit cross‐reactivity (types a and b), are the better urine‐based tests for Down syndrome screening. © 1997 by John Wiley & Sons, Ltd.

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Screening for Down syndrome using urine hCG free β-subunit in the second trimester of pregnancy

et al. 1997

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Stability of hCG free β-subunit and β-core fragment in urine

Cited by 8 publications

References 10 publications

Determination of hyperglycosylated human chorionic gonadotropin produced by malignant gestational trophoblastic neoplasias and male germ cell tumors using a lectin-based immunoassay and surface plasmon resonance

Determination of hyperglycosylated human chorionic gonadotropin produced by malignant gestational trophoblastic neoplasias and male germ cell tumors using a lectin-based immunoassay and surface plasmon resonance

Comparison of 12 assays for detecting hCG and related molecules in urine samples from Down syndrome pregnancies

Screening for Down syndrome using urine hCG free β-subunit in the second trimester of pregnancy

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