Stability of Human Salivary Lactate Dehydrogenase in the Present of Ethylenediaminetetraacetic Acid, Glycerol and Polyethylene Glycol at Various Temperatures: Preliminary Study

Rohaya, M. A. W.; Sahidan, S.; Zaidah, Z. A.; Zaman, H. Fahrul; Nuraliza, A. W.; Hisham, Z. A. Shahrul

doi:10.3923/jbs.2010.520.525

Cited by 7 publications

(12 citation statements)

References 13 publications

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“…The present study was in line with research stated that increment of lactate dehydrogenase activity is often caused by gingivitis and periodontitis respectively. 6 This research showed that the activity of LDH increased days by orthodontic force application. This features might occur because the initial wave of absorption is taken place from 3-5 days after the application of orthodontic force.…”

Section: Discussionmentioning

confidence: 92%

“…One of the metabolic changes is an increase activity of lactate dehydrogenase (LDH) which can be detected in saliva. [4][5][6][7] Lactate dehydrogenase is an enzyme normally present in the cytoplasm and release extracellularly when cells undergoing cell death. An increase of lactate dehydrogenase level have been reported during bone remodeling due to orthodontic treatment.…”

Section: Introductionmentioning

confidence: 99%

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Orthodontic Force Application in Correlation with Salivary Lactate Dehydrogenase Activity

Husin¹,

Tjandrawinata²,

Juliani³

et al. 2013

J Dent Indones

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Orthodontic tooth movement generate mechanical forces to periodontal ligament and alveolar bone. The forces correlate with initial responses of periodontal tissues and involving many metabolic changes. One of the metabolic changes detected in saliva is lactate dehydrogenase (LDH) activity. Objectives: To evaluate the correlation between orthodontic interrupted force application, lactate dehydrogenase activity and the distance of tooth movement. Methods: upper premolar, pre-retraction of upper canine and 1, 7, 14, 21 and 28 days post-retraction of upper canine with 100g interrupted orthodontic force. Results: duration of force (F=11.926;; p 14 and 28 days post-retraction of canine. The region of retraction correlated with the distance of tooth movement (F=7.377;; p=0.007). The duration of force correlated with the distance of tooth movement (F=66.554;; p=0.000). retraction of canine. Conclusion: This study concluded that orthodontic interrupted force application on canine could increase the distance of tooth movement and LDH activity in saliva. ABSTRAK Aplikasi gaya ortodonti terhadap aktivitas laktat dehidrogenase dalam saliva. Pergerakan gigi secara ortodonti menghasilkan gaya-gaya mekanik pada jaringan periodontal dan tulang alveolar. Gaya-gaya mekanik ini berhubungan dengan respon awal pada jaringan periodontium and terjadi banyak perubahan-perubahan metabolik. Salah satu perubahan metabolik yang dapat terjadi adalah peningkatan aktivitas enzim laktat dehidrogenase yang dapat dideteksi dalam saliva. Tujuan: Mengetahui korelasi antara pemberian gaya ortodonti dan aktivitas laktat dehidrogenase dalam saliva serta jarak pergerakan gigi. Metode: Penelitian ini mengunakan 140 sampel saliva dari 20 subjek, yaitu saliva yang diambil sebelum pencabutan gigi premolar, sebelum penarikan gigi kaninus rahang atas, 1, 7, 14, 21, dan 28 hari setelah penarikan gigi kaninus dengan gaya ortodonti interrupted sebesar 100g. Hasil: Analisis ANOVA menunjukkan adanya perbedaan yang bermakna antara aktivitas laktat dehidrogenase dengan durasi pemberian gaya (F=11,926;; p=0,000). Perbedaan bermakna juga ditunjukkan pada waktu sebelum dilakukan penarikan gigi kaninus dengan kelompok waktu 14 dan 28 hari. Terdapat kemaknaan antara regio penarikan berpengaruh terhadap jarak pergerakan (F=7,377;; p=0,007), dan terdapat pengaruh antara durasi pemberian gaya terhadap jarak pergerakan gigi (F=66,554;; p=0,000). Perbedaan bermakna diperlihatkan antara kelompok sebelum penarikan gigi kaninus terhadap kelompok 1, 7, 14, 21, dan 28 hari setelah penarikan gigi kaninus. Simpulan: Aplikasi gaya ortodonti pada gigi kaninus dapat meningkatkan jarak pergerakan gigi dan aktivitas laktat dehidrogenase dalam saliva.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Orthodontic Force Application in Correlation with Salivary Lactate Dehydrogenase Activity

Husin¹,

Tjandrawinata²,

Juliani³

et al. 2013

J Dent Indones

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“…Rohaya et al. investigated LDH stability at room temperature, 4 °C and −20 °C in presence of three different protectors [25]. The addition of PEG prevented degradation for two weeks at all three temperatures with a recovery of ≥98 %, while glycerol seemed to be suitable only for frozen samples and ethylenediaminetetraacetic acid (EDTA) only for room temperature.…”

Section: Discussionmentioning

confidence: 99%

“…Sodium bicarbonate (Sigma Aldrich Chemie GmbH, Taufkirchen, Germany), glucose (VWR International GmbH, Darmstadt, Germany) and gentamycin sulfate (Merck Millipore, Darmstadt, Germany) were added to the solution. PEG 400 (VWR International GmbH, Darmstadt, Germany) was selected as cryoprotector [25]. For LDH measurement, the Cytotoxicity Detection Kit PLUS (Roche Diagnostics GmbH, Mannheim, Germany) was used.…”

Section: Methodsmentioning

confidence: 99%

Validation and stability analysis of a modified lactate dehydrogenase (LDH) test method to be employed for an in vitro viable skin model

Bauhammer¹,

Sacha²,

Haltner³

2019

Heliyon

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In view of increasing numbers of dermatological disorders, transdermal drug delivery along with in vitro research is becoming increasingly popular. Herefore, qualified in vitro skin models are required. The objective of this study was the optimization and validation of a modified lactate dehydrogenase (LDH) release assay during the establishment of an in vitro viable human skin model, employable for a variety of skin associated disorders. Firstly, the most suitable LDH isoform for the study was determined. Subsequently, a stability study was conducted to investigate the best storage conditions of the LDH enzyme. Finally, the test system was validated in terms of linear range, range limits and system suitability. The results indicate LDH-5 as most suitable isoform due to its predominance in skin. The stability samples stored at −20 °C in the presence of polyethylene glycol (PEG) as cryoprotector displayed the targeted recovery of 100% ± 15 % until the end of the four-week study in contrast to other investigated conditions. A six-point calibration without PEG and a seven-point calibration with PEG including evaluation of system suitability and quantification limits were established with both correlation coefficients r 2 above 0.99 and all deviations below 15%. Concluding from those results, this method can be considered valid and useful for its employment in viability determination of viable in vitro skin models.

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“…A biomarker Lactate dehydrogenase LDH was determined according to De la Pena et al; Rohaya et al [17,18]. Also, routine liver function test parameters aspartate aminotransferase AST, alanine aminotransferase ALT, GGT and Albumin were relevant for monitoring chemotherapy hepatotoxic effects, were performed before and after the completion of the treatment.…”

Section: Biochemical Parametersmentioning

confidence: 99%

Biological Evidences of Hepatocellular Carcinoma Treatment with 1,3,4-oxadiazole-2-thiol as Anticancer Agent

Gabry¹,

Elgemeie²,

Basseli³

et al. 2017

J Biomol Res Ther

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Stability of Human Salivary Lactate Dehydrogenase in the Present of Ethylenediaminetetraacetic Acid, Glycerol and Polyethylene Glycol at Various Temperatures: Preliminary Study

Cited by 7 publications

References 13 publications

Orthodontic Force Application in Correlation with Salivary Lactate Dehydrogenase Activity

Orthodontic Force Application in Correlation with Salivary Lactate Dehydrogenase Activity

Validation and stability analysis of a modified lactate dehydrogenase (LDH) test method to be employed for an in vitro viable skin model

Biological Evidences of Hepatocellular Carcinoma Treatment with 1,3,4-oxadiazole-2-thiol as Anticancer Agent

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