Stability of inflammation markers in human blood collected using volumetric absorptive microsampling (VAMS) under typical laboratory storage temperatures

Establishing Personalized Blood Protein Reference Ranges Using Noninvasive Microsampling and Targeted Proteomics: Implications for Antidoping Strategies

“…We are in the process of greatly expanding the number of targets measured from undepleted blood samples with next-generation MS instrumentation and also improving our analytical robustness and throughput through the adoption of modern microflow LC systems more amenable to high-throughput clinical analysis (e.g., Evosep One, etc.). Additionally, improvements in remote or at-home microsampling techniques, including the use of wearable dried blood collection devices, should be implemented in future studies because they have the potential to dramatically increase study scope and scale by facilitating sample collection and allowing for greater frequency of sampling. − Future work should establish whether an athlete’s “bio-signature” could be established from such a panel using a much larger cohort of study participantsand whether clinical correlations could be made with health status or could be used to confirm illicit practices (i.e., substances of abuse, blood doping).…”

“…Additionally, improvements in remote or at-home microsampling techniques, including the use of wearable dried blood collection devices, should be implemented in future studies because they have the potential to dramatically increase study scope and scale by facilitating sample collection and allowing for greater frequency of sampling. 38 − 40 Future work should establish whether an athlete’s “bio-signature” could be established from such a panel using a much larger cohort of study participants—and whether clinical correlations could be made with health status or could be used to confirm illicit practices (i.e., substances of abuse, blood doping).…”

Portable Mid-Infrared Spectroscopy Combined with Chemometrics to Diagnose Fibromyalgia and Other Rheumatologic Syndromes Using Rapid Volumetric Absorptive Microsampling

“…The viscosity of the blood sample is greatly influenced by hematocrit levels that define the concentration of red blood cells in a unit volume. Samples with varying hematocrits can exhibit differences in diffusion rates when applied to filter cards, resulting in heterogenous volumes within the pre-defined circles [ 16 ]. The Neoteryx Mitra ® (Torrance, CA, USA) device is based on a novel volumetric absorptive microsampling (VAMS) approach for blood sampling.…”

“…In VAMS technology, a fixed volume of blood is absorbed onto a porous substrate; the volume absorbed is dependent on the characteristics and concentration of the substrate [ 17 ]. The Mitra sampler device comprises two plastic spindles, each with an adsorbent VAMS tip that wicks a fixed volume of blood [ 16 ]. The sampler is then placed in a protective plastic clamshell and locked in specimen bag with desiccant [ 18 ].…”

“…The sampler is then placed in a protective plastic clamshell and locked in specimen bag with desiccant [ 18 ]. The VAMS-based microsampling approach offers several advantages over DBS, including reduced sample volume requirements, easy transportation and storage, and controlled sample volume bias [ 16 , 19 , 20 ].…”