Stability of Insect Viruses in the Environment

Jaques, R. P.

doi:10.1016/b978-0-12-470295-0.50015-x

Cited by 47 publications

(40 citation statements)

References 217 publications

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“…It is possible that a less intense rainfall than 5 mm in 2 min would have resulted in significantly less viral transport. Once OB were splashed onto the plant, additional precipitation probably did not wash them away, because several studies have indicated that rain does not remove viral OB from leaf surfaces (18).…”

Section: Discussionmentioning

confidence: 99%

Quantification of Soil-to-Plant Transport of Recombinant Nucleopolyhedrovirus: Effects of Soil Type and Moisture, Air Currents, and Precipitation

Fuxa

Richter

2001

Appl Environ Microbiol

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Section: Discussionmentioning

confidence: 99%

Quantification of Soil-to-Plant Transport of Recombinant Nucleopolyhedrovirus: Effects of Soil Type and Moisture, Air Currents, and Precipitation

Fuxa

Richter

2001

Appl Environ Microbiol

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“…The deleterious affects of temperature on virus persistence is significant in storage and in the environment, but is comparably less important than solar radiation (Jacques, 1985). Inactivation by heat is a nonselective process and affects various functions associated with the virus.…”

Section: 2mentioning

confidence: 99%

Review of Vaccinia Virus and Baculovirus Viability Versus Virucides

Buckley¹,

O’Connell²,

Ouellette³

2008

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“…In general, the entomopathogenic viruses are very stable at low temperatures. Viruses can remain highly viable for several years, especially those with intact inclusion bodies stored in insect cadavers, dry powders or in suspensions sheltered from light and kept at 0-4°C (David & Gardiner 1967, Dulmage & Burgerjon 1977, Jacques 1985. On the other hand, the free particle viruses are less stable even at low temperatures.…”

mentioning

confidence: 99%

“…Laboratory and field studies suggest that the solar radiation, especially the ultraviolet portion of the spectrum, is probably the most important factor affecting the persistence of microbial insecticides. This radiation directly affects the nucleic acids, modifying or denaturing them, preventing growth and reproduction of the microorganism (Ignoffo et al 1977, Jacques 1985, Pawar et al 1995. In general, the entomopathogenic viruses are very stable at low temperatures.…”

mentioning

confidence: 99%

Stability and Persistence of Two Formulations Containing Anticarsia gemmatalis Nuclear Polyhedrovirus (AgMNPV)

Filho¹,

Alves

Augusto³

et al. 2001

Neotrop. Entomol.

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estudadas. As formulações foram armazenadas em condições ambiente e expostas à radiação ultravioleta em condições de laboratório e à radiação solar em condições de campo. A formulação pó molhável (PM) foi preparada através da impregnação da suspensão de vírus e adjuvantes em inerte mineral (Caolin). A formulação óleo emulsionável (OE) foi preparada pela mistura da suspensão do patógeno com óleo + adjuvantes. Durante 20 meses, com intervalos de 120 dias, amostras das formulações e do vírus original eram adicionadas à dieta artificial de lagartas de quarto ínstar de Anticarsia gemmatalis (Hübner). Após 20 meses de armazenamento, a formulação OE perdeu apenas 18,3% de sua atividade original, enquanto a formulação PM teve reduzida sua eficiência para 11,7% após 12 meses. Nenhuma diferença significativa foi observada para as duas formulações quando expostas à luz ultravioleta. Ambas protegeram o vírus quando comparado ao tratamento controle. Em condições de campo a formulação OE teve melhor persistência com cerca de 60% da atividade original presente após 14 dias da aplicação. Grande parte da formulação PM foi removida das folhas pela chuva. PALAVRAS-CHAVE: Insecta, nucleopoliedrovirus, armazenamento e radiação.ABSTRACT -The stability and the persistence of two AgMNPV formulations developed by the Instituto Biológico and the Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Brazil were studied. Formulations were stored in environmental conditions and exposed to ultraviolet radiation in laboratory and to solar radiation in the field. The wettable powder formulation (WP) was prepared by impregnating viral suspensions + adjuvants on inert mineral, chiefly kaolin. The emulsifiable oil formulation (EO) was prepared by mixing the pathogen suspension with corn oil + adjuvants. At 120-day intervals for 20 months, samples of formulations and the standard treatment (crude virus) kept in a laboratory cabinet were added to artificial diet fed to 4 th -instar Anticarsia gemmatalis (Hübner) larvae. After 20 months in storage, the formulation EO lost only 18.3% of its original activity, whereas the formulation WP had its efficiency reduced to 11.7% after 12 months. No significant differences between the two formulations were observed when exposed to the ultraviolet germicidal light, and both formulations protected the virus when compared to the standard treatment. Under field conditions, the EO formulation enhanced the viral persistence, with >60% of the original activity present 14 days after the application. Most of the WP formulation was removed from leaves by the rain.

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Stability of Insect Viruses in the Environment

Cited by 47 publications

References 217 publications

Quantification of Soil-to-Plant Transport of Recombinant Nucleopolyhedrovirus: Effects of Soil Type and Moisture, Air Currents, and Precipitation

Quantification of Soil-to-Plant Transport of Recombinant Nucleopolyhedrovirus: Effects of Soil Type and Moisture, Air Currents, and Precipitation

Review of Vaccinia Virus and Baculovirus Viability Versus Virucides

Stability and Persistence of Two Formulations Containing Anticarsia gemmatalis Nuclear Polyhedrovirus (AgMNPV)

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