Stability of interleukin-1β, -4, -6, -8, -10, -13, interferon-γ and tumor necrosis factor-α in human sera after repetitive freeze-thaw cycles and long storage

Kordulewska, Natalia; Topa, Justyna; Tańska, Małgorzata; Cieślińska, Anna; Fiedorowicz, Ewa; Jarmołowska, Beata

doi:10.1016/j.jpba.2021.113900

Cited by 12 publications

(15 citation statements)

References 25 publications

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“…This is similar to previous work which found human IL-1β concentration was not affected by up to 6 freeze/thaw cycles ( 37 ). Conversely, IL-1β concentrations decreased in samples that underwent 2 freeze/thaw cycles in another study ( 36 ). No difference in TNF-α concentration occurred between freeze/thaw cycles in the current study.…”

Section: Discussionmentioning

confidence: 92%

“…The long-term storage (18 months) of samples prior to analysis may have affected cytokine concentrations. While human cytokines when stored at −80°C remain stable for up to 2 years ( 39 ), levels decrease after 8 days when stored at −20°C ( 36 ). Unfortunately, no comparative investigations have been performed in horses to establish if the same holds true.…”

Section: Discussionmentioning

confidence: 99%

“…No difference in TNF-α concentration occurred between freeze/thaw cycles in the current study. Tumor necrosis factor α appears to be resilient to freeze/thaw processes; in previous studies, TNF-α concentration was unaffected in up to 6 and 10 freeze/thaw cycles (36,38). The influence of TNF-α and IL-1β concentration within an ACS product on therapeutic effect has not been quantified in the horse (24).…”

Section: Effect Of Freeze/thaw Cycles On Cytokine Concentrationmentioning

confidence: 94%

“…Unlike IL-1Ra, no difference in IL-10 concentration between the number of freeze/thaw cycles was found in this study. In human studies, there is contrasting evidence of the thermal stability of IL-10, with reports of concentration depletion following multiple freeze/thaw cycles (33,35), whilst other authors found evidence of thermal stability (36). The importance of IL-10 concentration on the therapeutic effect of ACS is unknown (24).…”

Section: Effect Of Freeze/thaw Cycles On Cytokine Concentrationmentioning

confidence: 99%

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Effects of Production Method and Repeated Freeze Thaw Cycles on Cytokine Concentrations and Microbial Contamination in Equine Autologous Conditioned Serum

Hale

Hughes

et al. 2021

Front. Vet. Sci.

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Autologous conditioned serum (ACS) is a common intra-articular treatment for osteoarthritis in horses. The objective of this study was to investigate the influence of ACS preparation method on product contamination and concentrations of relevant cytokines and the influence of multiple freeze/thaw cycles. Blood was obtained from 10 healthy Thoroughbred horses and processed in parallel using a commercial and a non-commercial method to obtain ACS. Fluorescent microsphere immunoassay (FMIA) analysis was performed to quantify Interleukin 1 receptor antagonist (IL-1Ra), Interleukin-10 (IL-10), Interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) concentrations in ACS obtained by both production methods. Effect of 3, 4 and 5 freeze/thaw cycles on concentrations of IL-1Ra, IL-10, IL-1β and TNF-α were assessed against baseline samples (2 cycles) in commercial ACS products. Standard aerobic and anaerobic culture methods were applied to both ACS products. Mixed effect one-way analyses of variance (ANOVA) were used to compare the two ACS production method for each cytokine. Repeated measures, mixed effect ANOVA were used to assess the effect of freeze/thaw on cytokine concentrations. Significance was set at P < 0.05. There was no difference in cytokine concentration between production methods (IL-1Ra P = 0.067, IL-1β P = 0.752, IL-10 P = 0.211 and TNF-α P = 0.25). Microbial growth was only observed in two samples obtained using the commercial production method. When compared to baseline, IL-1Ra concentration was decreased following the 5th freeze/thaw cycle (P < 0.001). These results suggest that the concentration of important cytokines are not influenced by ACS production method. When storing ACS samples for future use, freeze/thaw cycles associated with standard clinical practice are unlikely to influence cytokine concentrations. However, the lack of outcome measures associated with 1 or 2 freeze/thaw cycles represents a limitation of this study.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Effect Of Freeze/thaw Cycles On Cytokine Concentrationmentioning

confidence: 94%

Section: Effect Of Freeze/thaw Cycles On Cytokine Concentrationmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Production Method and Repeated Freeze Thaw Cycles on Cytokine Concentrations and Microbial Contamination in Equine Autologous Conditioned Serum

Hale

Hughes

et al. 2021

Front. Vet. Sci.

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“…Common to most of the cytokines, IL-10 has a very short half-life and narrow therapeutic windows. 14 Therefore, limited therapeutic effects would be achieved by local random injection of IL-10 at periodontal defect sites, and multiple injections may bring much suffering to patients. A strategy for achieving local sustained release of IL-10 within the defect sites of the diabetic alveolar bone is anticipated.…”

Section: ■ Introductionmentioning

confidence: 99%

Physically Cross-Linked DNA Hydrogel-Based Sustained Cytokine Delivery for In Situ Diabetic Alveolar Bone Rebuilding

Wang

et al. 2022

ACS Appl. Mater. Interfaces

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The development of a biodegradable and shape-adaptable bioscaffold that can enhance local cytokine retention and bioactivity is essential for the application of immunotherapy in periodontal diseases. Here, we report a biodegradable, anti-inflammatory, and osteogenic ILGel that uses a physically cross-linked DNA hydrogel as a soft bioscaffold for the long-term sustained release of cytokine interleukin-10 (IL-10) to accelerate diabetic alveolar bone rebuilding. Porous microstructures of ILGel favored the encapsulation of IL-10 and maintained IL-10 bioactivity for at least 7 days. ILGel can be gradually degraded or hydrolyzed under physiological conditions, avoiding the potential undesired side effects on dental tissues. Long-term sustained release of bioactive IL-10 from ILGel not only promoted M2 macrophage polarization and attenuated periodontal inflammation but also triggered osteogenesis of mesenchymal stem cells (MSCs), leading to accelerated alveolar bone formation and healing of alveolar bone defects under diabetic conditions in vivo. ILGel treatment significantly accelerated the defect healing rate of diabetic alveolar injury up to 93.42 ± 4.6% on day 21 post treatment compared to that of free IL-10 treatment (63.30 ± 7.39%), with improved trabecular architectures. Our findings imply the potential application of the DNA hydrogel as the bioscaffold for cytokine-based immunotherapy in diabetic alveolar bone injury and other periodontal diseases.

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A novel case of impaired C-reactive protein response following open-heart surgery: A case report and review of the literature

Vinnes

Lillemoen

Persson

et al. 2021

Clinica Chimica Acta

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Stability of interleukin-1β, -4, -6, -8, -10, -13, interferon-γ and tumor necrosis factor-α in human sera after repetitive freeze-thaw cycles and long storage

Cited by 12 publications

References 25 publications

Effects of Production Method and Repeated Freeze Thaw Cycles on Cytokine Concentrations and Microbial Contamination in Equine Autologous Conditioned Serum

Effects of Production Method and Repeated Freeze Thaw Cycles on Cytokine Concentrations and Microbial Contamination in Equine Autologous Conditioned Serum

Physically Cross-Linked DNA Hydrogel-Based Sustained Cytokine Delivery for In Situ Diabetic Alveolar Bone Rebuilding

A novel case of impaired C-reactive protein response following open-heart surgery: A case report and review of the literature

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